Ozone (O3) is ubiquitous in the environment and exposure to high levels of O3 has been associated with various respiratory diseases such as asthma, emphysema, bronchitis and so on. Therefore, it is necessary to develop a simple and efficient detection method for monitoring O3 in the environment and living cells. In this study, an intramolecular charge transfer (ICT) compound was designed and synthesized based on a phenol-type hemicyanine dye and 4-bromo-1-butene, which could specifically detect O3 in aqueous solution. Due to the ICT process, the absorption spectrometry, fluorescence spectrometry and color of the probe hemicyanine-butyl-3-enyl (HCB) changes significantly and thus the rapid and sensitive detection of O3 was realized. The interaction between O3 and the probe HCB can be completed within 40 min, a the detection limit of O3 can be as low as 2.15×10−7 mol/L. Finally, the proposed method has been successfully applied to the visual detection of O3 in simulated O3 environment and living cells.

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