The parasite Toxoplasma gondii infects various animals and can cause serious opportunistic infections in immunocompromised humans or neonates. Motility is of great importance for parasite biology and virulence. During the tachyzoite stage, the parasite uses gliding motility to invade, egress from and move between host cells. Motility is driven by a myosin motor protein, termed myosinA (MyoA). Given that the MyoA motor complex is highly conserved in apicomplexan parasites and differs from myosins found in humans, it represents a potential drug target.