Fluorescence enhancement of flavonoids and its application in the ingredient determination for some traditional Chinese medicines by CE-LIF

Flavonoids are widely used in the treatment of various diseases due to their antioxidant, anti-inflammatory, anticancer and antiviral properties. Fluorescence detection is rarely applied for the determination of flavonoids because of their weak fluorescence. In this work, a method of fluorescence enhancement of flavonoids was firstly introduced by using sodium acetate for flavonoid derivatization. The study discovered that flavonoids, with a hydroxyl at the C3 position, had the ability to emit strong fluorescence after derivatization. Five flavonoids including kaempferide, galangin, isorhamnetin, kaempferol and quercetin, having the special structure, were selected, derivatized and analyzed by capillary electrophoresis with laser-induced fluorescence detection. Under the optimal conditions, the five flavonoids could be completely separated within 3 minutes. Good linear relationships were obtained for all analytes and the limit of detections for the five flavonoids were in the range of 1.18-4.67 × 10-7 mol/L. Finally, the method was applied to the determination of flavonoids in five traditional Chinese medicines including asters, chamomile, galangal, tangerine peel and cacumen biotae. Flavonoids were successfully found from all these medicines by the developed method. The recoveries were in the range of 84.2%-111%. The method developed in this study was fast, sensitive and reliable for the determination of flavonoids.

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