Validated ion exchange HPLC method for the quantification of levothyroxine – a narrow therapeutic index drug – used for the treatment of hypothyroidism

Article title

Abstract

Keywords

Introduction

Methodology

Experimental preparation of the mobile phase

Preparation of 0.05% methanolic Sodium Hydroxide

Preparation of LT4 standard solution (200 µg/mL)

Chromatographic conditions

Construction of calibration graph

Pharmaceutical tablets

Results and discussion

Optimization of chromatographic conditions

Detection wavelength

Column type

Mobile phase (composition, ratio, aqueous phase)

Flow rate

Method validation and statistical analysis

System suitability

Method validation

Linearity and concentration range

Limit of detection and Limit of quantification

Accuracy

Precision

Specificity

Robustness

Pharmaceutical application

Conclusion

References

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