Bucciarelli, A. & Motta, A. Use of Bombyx mori silk fibroin in tissue engineering: from cocoons to medical devices, challenges, and future perspectives. Biomater. Adv. 139, 212982 (2022).

Article  CAS  PubMed  Google Scholar 

Li, C. et al. Design of biodegradable, implantable devices towards clinical translation. Nat. Rev. Mater. 5, 61–81 (2020).

Article  Google Scholar 

Li, C. et al. Fiber‐based biopolymer processing as a route toward sustainability. Adv. Mater. 34, 2105196 (2022).

Article  CAS  Google Scholar 

Marelli, B. Biomaterials for boosting food security. Science 376, 146–147 (2022).

Article  CAS  PubMed  Google Scholar 

Post, M. J. et al. Scientific, sustainability and regulatory challenges of cultured meat. Nat. Food 1, 403–415 (2020).

Article  Google Scholar 

Vepari, C. & Kaplan, D. L. Silk as a biomaterial. Prog. Polym. Sci. 32, 991–1007 (2007).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Kundu, S. C., Dash, B. C., Dash, R. & Kaplan, D. L. Natural protective glue protein, sericin bioengineered by silkworms: potential for biomedical and biotechnological applications. Prog. Polym. Sci. 33, 998–1012 (2008).

Article  CAS  Google Scholar 

Omenetto, F. G. & Kaplan, D. L. New opportunities for an ancient material. Science 329, 528–531 (2010).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Department Of Health And Human Services. Code of Federal Regulations Title 21: Natural nonabsorbable silk surgical suture. FDA https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/cfrsearch.cfm?fr=878.5030 (2023).

Melke, J., Midha, S., Ghosh, S., Ito, K. & Hofmann, S. Silk fibroin as biomaterial for bone tissue engineering. Acta Biomater. 31, 1–16 (2016).

Article  CAS  PubMed  Google Scholar 

Holland, C., Numata, K., Rnjak‐Kovacina, J. & Seib, F. P. The biomedical use of silk: past, present, future. Adv. Healthc. Mater. 8, 1800465 (2019).

Article  Google Scholar 

Janani, G. et al. Insight into silk-based biomaterials: from physicochemical attributes to recent biomedical applications. ACS Appl. Bio Mater. 2, 5460–5491 (2019).

Article  CAS  PubMed  Google Scholar 

Fine, N. A. et al. SERI surgical scaffold, prospective clinical trial of a silk-derived biological scaffold in two-stage breast reconstruction: 1-year data. Plast. Reconstr. Surg. 135, 339–351 (2015).

Article  CAS  PubMed  Google Scholar 

Gulka, C. P. et al. A novel silk‐based vocal fold augmentation material: 6‐month evaluation in a canine model. Laryngoscope 129, 1856–1862 (2019).

Article  CAS  PubMed  Google Scholar 

Brown, J. E. et al. Injectable silk protein microparticle-based fillers: a novel material for potential use in glottic insufficiency. J. Voice 33, 773–780 (2019).

Article  PubMed  Google Scholar 

Levin, B., Rajkhowa, R., Redmond, S. L. & Atlas, M. D. Grafts in myringoplasty: utilizing a silk fibroin scaffold as a novel device. Expert. Rev. Med. Device 6, 653–664 (2009).

Article  CAS  Google Scholar 

Koh, L.-D. et al. Structures, mechanical properties and applications of silk fibroin materials. Prog. Polym. Sci. 46, 86–110 (2015).

Article  CAS  Google Scholar 

Guo, C., Li, C. & Kaplan, D. L. Enzymatic degradation of Bombyx mori silk materials: a review. Biomacromolecules 21, 1678–1686 (2020).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Leal‐Egaña, A. & Scheibel, T. Silk‐based materials for biomedical applications. Biotechnol. Appl. Biochem. 55, 155–167 (2010).

Article  PubMed  Google Scholar 

Rockwood, D. N. et al. Materials fabrication from Bombyx mori silk fibroin. Nat. Protoc. 6, 1612 (2011).

Article  CAS  PubMed  Google Scholar 

Wu, J., Sahoo, J. K., Li, Y., Xu, Q. & Kaplan, D. L. Challenges in delivering therapeutic peptides and proteins: a silk-based solution. J. Control. Release 345, 176–189 (2022).

Article  CAS  PubMed  Google Scholar 

Falcucci, T. et al. Degradable silk‐based subcutaneous oxygen sensors. Adv. Funct. Mater. 32, 2202020 (2022). This study demonstrates a degradable oxygen-sensing platform based on silk fibroin.

Article  CAS  Google Scholar 

Kasoju, N. & Bora, U. Silk fibroin in tissue engineering. Adv. Healthc. Mater. 1, 393–412 (2012).

Article  CAS  PubMed  Google Scholar 

Zhang, W. et al. Silk fibroin biomaterial shows safe and effective wound healing in animal models and a randomized controlled clinical trial. Adv. Healthc. Mater. 6, 1700121 (2017).

Article  Google Scholar 

Farokhi, M., Mottaghitalab, F., Fatahi, Y., Khademhosseini, A. & Kaplan, D. L. Overview of silk fibroin use in wound dressings. Trends Biotechnol. 36, 907–922 (2018).

Article  CAS  PubMed  Google Scholar 

Wenk, E., Merkle, H. P. & Meinel, L. Silk fibroin as a vehicle for drug delivery applications. J. Control. Release 150, 128–141 (2011).

Article  CAS  PubMed  Google Scholar 

Agostinacchio, F., Mu, X., Dirè, S., Motta, A. & Kaplan, D. L. In situ 3D printing: opportunities with silk inks. Trends Biotechnol. 39, 719–730 (2021).

Article  CAS  PubMed  Google Scholar 

Chakraborty, J., Mu, X., Pramanick, A., Kaplan, D. L. & Ghosh, S. Recent advances in bioprinting using silk protein-based bioinks. Biomaterials 287, 121672 (2022).

Article  CAS  PubMed  Google Scholar 

Chawla, S., Midha, S., Sharma, A. & Ghosh, S. Silk‐based bioinks for 3D bioprinting. Adv. Healthc. Mater. 7, 1701204 (2018).

Article  Google Scholar 

Hasturk, O. et al. Cytoprotection of human progenitor and stem cells through encapsulation in alginate templated, dual crosslinked silk and silk–gelatin composite hydrogel microbeads. Adv. Healthc. Mater. 11, 2200293 (2022).

Article  CAS  Google Scholar 

Murphy, A. R. & Kaplan, D. L. Biomedical applications of chemically-modified silk fibroin. J. Mater. Chem. 19, 6443–6450 (2009).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Ha, S.-W., Gracz, H. S., Tonelli, A. E. & Hudson, S. M. Structural study of irregular amino acid sequences in the heavy chain of Bombyx mori silk fibroin. Biomacromolecules 6, 2563–2569 (2005).

Article  CAS  PubMed  Google Scholar 

Wang, Q., Chen, Q., Yang, Y. & Shao, Z. Effect of various dissolution systems on the molecular weight of regenerated silk fibroin. Biomacromolecules 14, 285–289 (2013). This study summarizes the effect of various degumming and dissolution processes on silk chain degradation and MW.

Article  CAS  PubMed  Google Scholar 

Wang, F., Cao, T.-T. & Zhang, Y.-Q. Effect of silk protein surfactant on silk degumming and its properties. Mater. Sci. Eng. C 55, 131–136 (2015).

Article  CAS  Google Scholar 

Zhang, Y. A comparative study of silk degumming methods. Acta Sericol. Sin. 28, 75–79 (2002).

CAS  Google Scholar 

Yuksek, M., Kocak, D., Beyit, A. & Merdan, N. Effect of degumming performed with different type natural soaps and through ultrasonic method on the properties of silk fiber. Adv. Environ. Biol. 6, 801–808 (2012).

CAS  Google Scholar 

Ha, S.-W., Park, Y. H. & Hudson, S. M. Dissolution of Bombyx mori silk fibroin in the calcium nitrate tetrahydrate−methanol system and aspects of wet spinning of fibroin solution. Biomacromolecules 4, 488–496 (2003).

Article  CAS  PubMed  Google Scholar 

Um, I. C., Kweon, H. Y., Lee, K. G. & Park, Y. H. The role of formic acid in solution stability and crystallization of silk protein polymer. Int. J. Biol. Macromol. 33, 203–213 (2003).

Article  CAS  PubMed  Google Scholar 

Phillips, D. M. et al. Dissolution and regeneration of Bombyx mori silk fibroin using ionic liquids. J. Am. Chem. Soc. 126, 14350–14351 (2004).

Article  CAS  PubMed  Google Scholar 

Yao, J., Masuda, H., Zhao, C. & Asakura, T. Artificial spinning and characterization of silk fiber from Bombyx mori silk fibroin in hexafluoroacetone hydrate. Macromolecules 35, 6–9 (2002).

Article  CAS  Google Scholar 

Ha, S.-W., Tonelli, A. E. & Hudson, S. M. Structural studies of Bombyx mori silk fibroin during regeneration from solutions and wet fiber spinning. Biomacromolecules 6, 1722–1731 (2005).

Article  CAS  PubMed  Google Scholar 

Sahoo, J. K. et al. Silk degumming time controls horseradish peroxidase-catalyzed hydrogel properties. Biomater. Sci. 8, 4176–4185 (2020).