Tetracycline (TC) is an antibiotic used in veterinary medicine to treat animals. Despite this, TC is not readily absorbed by animals, most of which is released into the environment via animal feces and urine. The biological chain causes TC to accumulate in various organisms and eventually cause significant harm to humans. This study presents an electrochemical sensor for determining TC based on surface molecular imprinted polymer (SMIP). The mesoporous magnetic nanomaterials (Fe3O4@SiO2@mTiO2) possessing higher specific surface area were successfully fabricated and considered the imprinted support after being modified with dopamine functionalized reversible addition-fragmentation chain transfer (RAFT) agent. The magnetic molecular imprinted polymer (MSMIP) was finally prepared through a surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization in the presence of TC, followed by an optimization of the ratio of functional monomer to TC by density functional theory (DFT) simulation. The electrochemical sensor was successfully constructed by dropping the suspension of reduced graphene oxide and MSMIP on the glassy carbon electrode. Under the optimized conditions, the peak current was linear to TC concentration from 1.6×10-9 to 8.8×10-8 mol L-1 (R2 of 0.9996) and the detection limit was 9.16×10-10 mol L-1. TC could be measured quickly in real milk samples using the as-prepared electrochemical sensor, and recovery rates ranged from 96.27% to 103.84% when standards were added.

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