Brewer, G. J. & Torricelli, J. R. Isolation and culture of adult neurons and neurospheres. Nat. Protoc. 2, 1490–1498 (2007).

Article  CAS  PubMed  Google Scholar 

Crouch, E. E. & Doetsch, F. FACS isolation of endothelial cells and pericytes from mouse brain microregions. Nat. Protoc. 13, 738–751 (2018).

Article  CAS  PubMed  Google Scholar 

Guo, W., Patzlaff, N. E., Jobe, E. M. & Zhao, X. Isolation of multipotent neural stem or progenitor cells from both the dentate gyrus and subventricular zone of a single adult mouse. Nat. Protoc. 7, 2005–2012 (2012).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Valtcheva, M. V. et al. Surgical extraction of human dorsal root ganglia from organ donors and preparation of primary sensory neuron cultures. Nat. Protoc. 11, 1877–1888 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Gray, D. T. & Barnes, C. A. Experiments in macaque monkeys provide critical insights into age-associated changes in cognitive and sensory function. Proc. Natl Acad. Sci. USA 116, 26247–26254 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhou, Y. et al. Atypical behaviour and connectivity in SHANK3-mutant macaques. Nature 570, 326–331 (2019).

Article  CAS  PubMed  Google Scholar 

Qiu, P. et al. BMAL1 knockout macaque monkeys display reduced sleep and psychiatric disorders. Natl Sci. Rev. 6, 87–100 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Park, T. I.-H. et al. Routine culture and study of adult human brain cells from neurosurgical specimens. Nat. Protoc. 17, 190–221 (2022).

Article  CAS  PubMed  Google Scholar 

Park, T. I.-H. et al. Isolation and culture of functional adult human neurons from neurosurgical brain specimens. Brain Commun. 2, fcaa171 (2020).

Article  PubMed  PubMed Central  Google Scholar 

Nott, A., Schlachetzki, J. C. M., Fixsen, B. R. & Glass, C. K. Nuclei isolation of multiple brain cell types for omics interrogation. Nat. Protoc. 16, 1629–1646 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Lake, B. B. et al. Neuronal subtypes and diversity revealed by single-nucleus RNA sequencing of the human brain. Science 352, 1586–1590 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Habib, N. et al. Massively parallel single-nucleus RNA-seq with DroNc-seq. Nat. Methods 14, 955–958 (2017).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zhu, Y. et al. Spatiotemporal transcriptomic divergence across human and macaque brain development. Science 362, eaat8077 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Han, L. et al. Cell transcriptomic atlas of the non-human primate Macaca fascicularis. Nature 604, 723–731 (2022).

Article  CAS  PubMed  Google Scholar 

Mathys, H. et al. Single-cell transcriptomic analysis of Alzheimer’s disease. Nature 570, 332–337 (2019).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Franjic, D. et al. Transcriptomic taxonomy and neurogenic trajectories of adult human, macaque, and pig hippocampal and entorhinal cells. Neuron 110, 452–469.e14 (2022).

Article  CAS  PubMed  Google Scholar 

Ayhan, F. et al. Resolving cellular and molecular diversity along the hippocampal anterior-to-posterior axis in humans. Neuron 109, 2091–2105.e6 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tran, M. N. et al. Single-nucleus transcriptome analysis reveals cell-type-specific molecular signatures across reward circuitry in the human brain. Neuron 109, 3088–3103.e5 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Wei, J.-R. et al. Identification of visual cortex cell types and species differences using single-cell RNA sequencing. Nat. Commun. 13, 6902 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hao, Z.-Z. et al. Single-cell transcriptomics of adult macaque hippocampus reveals neural precursor cell populations. Nat. Neurosci. 25, 805–817 (2022).

Article  CAS  PubMed  Google Scholar 

Zhong, S. et al. Decoding the development of the human hippocampus. Nature 577, 531–536 (2020).

Article  CAS  PubMed  Google Scholar 

Nowakowski, T. J. et al. Spatiotemporal gene expression trajectories reveal developmental hierarchies of the human cortex. Science 358, 1318–1323 (2017).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Schmitz, M. T. et al. The development and evolution of inhibitory neurons in primate cerebrum. Nature 603, 871–877 (2022).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Jiang, X. et al. Principles of connectivity among morphologically defined cell types in adult neocortex. Science 350, aac9462 (2015).

Article  PubMed  PubMed Central  Google Scholar 

Ting, J. T. et al. Preparation of acute brain slices using an optimized N-methyl-d-glucamine protective recovery method. J. Vis. Exp. 26, 53825 (2018).

Google Scholar 

Huang, W. et al. Linking transcriptomes with morphological and functional phenotypes in mammalian retinal ganglion cells. Cell Rep. 40, 111322 (2022).

Article  CAS  PubMed  Google Scholar 

Mich, J. K. et al. Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell Rep. 34, 108754 (2021).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Zeisel, A. et al. Molecular architecture of the mouse nervous system. Cell 174, 999–1014.e22 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Saunders, A. et al. Molecular diversity and specializations among the cells of the adult mouse brain. Cell 174, 1015–1030.e16 (2018).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Braidy, N. et al. Effects of kynurenine pathway metabolites on intracellular NAD synthesis and cell death in human primary astrocytes and neurons. Int. J. Tryptophan Res. 2, 61–69 (2009).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Bohár, Z., Toldi, J., Fülöp, F. & Vécsei, L. Changing the face of kynurenines and neurotoxicity: therapeutic considerations. Int. J. Mol. Sci. 16, 9772–9793 (2015).

Article  PubMed  PubMed Central  Google Scholar 

Kanwar, J. R., Kanwar, R. K. & Krissansen, G. W. Simultaneous neuroprotection and blockade of inflammation reverses autoimmune encephalomyelitis. Brain 127, 1313–1331 (2004).

Article  PubMed  Google Scholar 

Minnella, A. M. et al. Excitotoxic superoxide production and neuronal death require both ionotropic and non-ionotropic NMDA receptor signaling. Sci. Rep. 8, 17522 (2018).

Article  PubMed  PubMed Central  Google Scholar 

Lysko, P. G., Webb, C. L., Yue, T. L., Gu, J. L. & Feuerstein, G. Neuroprotective effects of tetrodotoxin as a Na+ channel modulator and glutamate release inhibitor in cultured rat cerebellar neurons and in gerbil global brain ischemia. Stroke 25, 2476–2482 (1994).

Article  CAS  PubMed  Google Scholar 

Tasic, B. et al. Adult mouse cortical cell taxonomy revealed by single cell transcriptomics. Nat. Neurosci. 19, 335–346 (2016).

Article  CAS  PubMed  PubMed Central  Google Scholar 

Hrvatin, S. et al. Single-cell analysis of experience-dependent transcriptomic states in the mouse visual cortex. Nat. Neurosci. 21, 120–129 (2018).

Article  CAS  PubMed  Google Scholar