Brewer, G. J. & Torricelli, J. R. Isolation and culture of adult neurons and neurospheres. Nat. Protoc. 2, 1490–1498 (2007).
Article CAS PubMed Google Scholar
Crouch, E. E. & Doetsch, F. FACS isolation of endothelial cells and pericytes from mouse brain microregions. Nat. Protoc. 13, 738–751 (2018).
Article CAS PubMed Google Scholar
Guo, W., Patzlaff, N. E., Jobe, E. M. & Zhao, X. Isolation of multipotent neural stem or progenitor cells from both the dentate gyrus and subventricular zone of a single adult mouse. Nat. Protoc. 7, 2005–2012 (2012).
Article CAS PubMed PubMed Central Google Scholar
Valtcheva, M. V. et al. Surgical extraction of human dorsal root ganglia from organ donors and preparation of primary sensory neuron cultures. Nat. Protoc. 11, 1877–1888 (2016).
Article CAS PubMed PubMed Central Google Scholar
Gray, D. T. & Barnes, C. A. Experiments in macaque monkeys provide critical insights into age-associated changes in cognitive and sensory function. Proc. Natl Acad. Sci. USA 116, 26247–26254 (2019).
Article CAS PubMed PubMed Central Google Scholar
Zhou, Y. et al. Atypical behaviour and connectivity in SHANK3-mutant macaques. Nature 570, 326–331 (2019).
Article CAS PubMed Google Scholar
Qiu, P. et al. BMAL1 knockout macaque monkeys display reduced sleep and psychiatric disorders. Natl Sci. Rev. 6, 87–100 (2019).
Article CAS PubMed PubMed Central Google Scholar
Park, T. I.-H. et al. Routine culture and study of adult human brain cells from neurosurgical specimens. Nat. Protoc. 17, 190–221 (2022).
Article CAS PubMed Google Scholar
Park, T. I.-H. et al. Isolation and culture of functional adult human neurons from neurosurgical brain specimens. Brain Commun. 2, fcaa171 (2020).
Article PubMed PubMed Central Google Scholar
Nott, A., Schlachetzki, J. C. M., Fixsen, B. R. & Glass, C. K. Nuclei isolation of multiple brain cell types for omics interrogation. Nat. Protoc. 16, 1629–1646 (2021).
Article CAS PubMed PubMed Central Google Scholar
Lake, B. B. et al. Neuronal subtypes and diversity revealed by single-nucleus RNA sequencing of the human brain. Science 352, 1586–1590 (2016).
Article CAS PubMed PubMed Central Google Scholar
Habib, N. et al. Massively parallel single-nucleus RNA-seq with DroNc-seq. Nat. Methods 14, 955–958 (2017).
Article CAS PubMed PubMed Central Google Scholar
Zhu, Y. et al. Spatiotemporal transcriptomic divergence across human and macaque brain development. Science 362, eaat8077 (2018).
Article CAS PubMed PubMed Central Google Scholar
Han, L. et al. Cell transcriptomic atlas of the non-human primate Macaca fascicularis. Nature 604, 723–731 (2022).
Article CAS PubMed Google Scholar
Mathys, H. et al. Single-cell transcriptomic analysis of Alzheimer’s disease. Nature 570, 332–337 (2019).
Article CAS PubMed PubMed Central Google Scholar
Franjic, D. et al. Transcriptomic taxonomy and neurogenic trajectories of adult human, macaque, and pig hippocampal and entorhinal cells. Neuron 110, 452–469.e14 (2022).
Article CAS PubMed Google Scholar
Ayhan, F. et al. Resolving cellular and molecular diversity along the hippocampal anterior-to-posterior axis in humans. Neuron 109, 2091–2105.e6 (2021).
Article CAS PubMed PubMed Central Google Scholar
Tran, M. N. et al. Single-nucleus transcriptome analysis reveals cell-type-specific molecular signatures across reward circuitry in the human brain. Neuron 109, 3088–3103.e5 (2021).
Article CAS PubMed PubMed Central Google Scholar
Wei, J.-R. et al. Identification of visual cortex cell types and species differences using single-cell RNA sequencing. Nat. Commun. 13, 6902 (2022).
Article CAS PubMed PubMed Central Google Scholar
Hao, Z.-Z. et al. Single-cell transcriptomics of adult macaque hippocampus reveals neural precursor cell populations. Nat. Neurosci. 25, 805–817 (2022).
Article CAS PubMed Google Scholar
Zhong, S. et al. Decoding the development of the human hippocampus. Nature 577, 531–536 (2020).
Article CAS PubMed Google Scholar
Nowakowski, T. J. et al. Spatiotemporal gene expression trajectories reveal developmental hierarchies of the human cortex. Science 358, 1318–1323 (2017).
Article CAS PubMed PubMed Central Google Scholar
Schmitz, M. T. et al. The development and evolution of inhibitory neurons in primate cerebrum. Nature 603, 871–877 (2022).
Article CAS PubMed PubMed Central Google Scholar
Jiang, X. et al. Principles of connectivity among morphologically defined cell types in adult neocortex. Science 350, aac9462 (2015).
Article PubMed PubMed Central Google Scholar
Ting, J. T. et al. Preparation of acute brain slices using an optimized N-methyl-d-glucamine protective recovery method. J. Vis. Exp. 26, 53825 (2018).
Huang, W. et al. Linking transcriptomes with morphological and functional phenotypes in mammalian retinal ganglion cells. Cell Rep. 40, 111322 (2022).
Article CAS PubMed Google Scholar
Mich, J. K. et al. Functional enhancer elements drive subclass-selective expression from mouse to primate neocortex. Cell Rep. 34, 108754 (2021).
Article CAS PubMed PubMed Central Google Scholar
Zeisel, A. et al. Molecular architecture of the mouse nervous system. Cell 174, 999–1014.e22 (2018).
Article CAS PubMed PubMed Central Google Scholar
Saunders, A. et al. Molecular diversity and specializations among the cells of the adult mouse brain. Cell 174, 1015–1030.e16 (2018).
Article CAS PubMed PubMed Central Google Scholar
Braidy, N. et al. Effects of kynurenine pathway metabolites on intracellular NAD synthesis and cell death in human primary astrocytes and neurons. Int. J. Tryptophan Res. 2, 61–69 (2009).
Article CAS PubMed PubMed Central Google Scholar
Bohár, Z., Toldi, J., Fülöp, F. & Vécsei, L. Changing the face of kynurenines and neurotoxicity: therapeutic considerations. Int. J. Mol. Sci. 16, 9772–9793 (2015).
Article PubMed PubMed Central Google Scholar
Kanwar, J. R., Kanwar, R. K. & Krissansen, G. W. Simultaneous neuroprotection and blockade of inflammation reverses autoimmune encephalomyelitis. Brain 127, 1313–1331 (2004).
Minnella, A. M. et al. Excitotoxic superoxide production and neuronal death require both ionotropic and non-ionotropic NMDA receptor signaling. Sci. Rep. 8, 17522 (2018).
Article PubMed PubMed Central Google Scholar
Lysko, P. G., Webb, C. L., Yue, T. L., Gu, J. L. & Feuerstein, G. Neuroprotective effects of tetrodotoxin as a Na+ channel modulator and glutamate release inhibitor in cultured rat cerebellar neurons and in gerbil global brain ischemia. Stroke 25, 2476–2482 (1994).
Article CAS PubMed Google Scholar
Tasic, B. et al. Adult mouse cortical cell taxonomy revealed by single cell transcriptomics. Nat. Neurosci. 19, 335–346 (2016).
Article CAS PubMed PubMed Central Google Scholar
Hrvatin, S. et al. Single-cell analysis of experience-dependent transcriptomic states in the mouse visual cortex. Nat. Neurosci. 21, 120–129 (2018).
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