A Validated HPLC Method for the Simultaneous Quantification of Curcumin, Silybin, and Psoralen: Application to Phytopharmaceuticals

The present study was aimed to develop a simple, sensitive, and precise reversed-phase HPLC method for the simultaneous separation and quantification of curcumin, silybin, and psoralen in phytopharmaceuticals. The developed method was optimized and validated as per International Conference on Harmonization guidelines. Separation and resolution were optimized using a C-18 column (250 × 4.6 mm, 5 µm) witha mobile phase composition of acetonitrile and 10 mM ammonium acetate in 0.1% formic acid. A gradient method with 11 min running time at a flow rate of 1.0 ml/min was used for the separation. The detection wavelengths and retention times of curcumin, silybin, psoralen, and telmisartan (internal standard) were found to be 421, 287, 245, and 254 nm, and 9.8, 8.3, 9.4, and 10.4 min, respectively. Linearity was established in both aqueous phase and plasma with R2 values > 0.99. In addition, the above-developed method was used for the screening of in-house hydrogel formulations containing curcumin, silybin, and psoralen.

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