Protective effects of grape seed proanthocyanidins on podocytes against injury induced by glucolipotoxicity

Please use this identifier to cite or link to this item: http://nopr.niscpr.res.in/handle/123456789/61647

metadata.dc.identifier.doi: https://doi.org/10.56042/ijeb.v61i04.177Title: Protective effects of grape seed proanthocyanidins on podocytes against injury induced by glucolipotoxicityAuthors: Li, Haiyan
Wang, Tongling
Liu, Dandan
Ding, Yusong
Liu, JinbaoKeywords: Diabetic nephropathy;Iron;NF-E2-Related Factor 2;Oxidative stressIssue Date: Apr-2023Abstract: Diabetic nephropathy (DN) is the most common cause of end-stage renal failure, oxidative damage and iron metabolism disorder are related to DN progression, but the exact pathogenesis remains unclear. Grape seed proanthocyanidins extract (GSPE) are one of the most widely distributed polyphenols with antioxidant properties. Here, we evaluated the mechanism by which GSPE alleviates podocyte damage induced by high glucose and palmitic acid (HG/PA). Podocytes were cultured in groups in normal environment; these were based on whether HG/PA and ML385 were present and the GSPE conc. 10, 25 and 50 mg/L. The flow cytometer, immunofluorescence staining, Western blot, quantitative real time-PCR (qRT-PCR), kit method and fluorescence microscope were used, respectively. After HG/PA intervention, the podocyte morphology was destroyed, the survival rate was reduced, the cell apoptosis was increased, and the fluorescence intensity of ROS detection was increased, iron metabolism was disordered. After adding ML385, increased oxidative damage to podocyte, nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream protein and mRNA expression levels were significantly reduced. However, application of different concentrations of GSPE could reverse these situations. Our findings indicate that GSPE regulates intracellular iron metabolism disorders and reduces the oxidative damage of podocytes possibly through activation of the Nrf2 signaling pathway.Page(s): 265-275ISSN: 0975-1009 (Online); 0019-5189 (Print)Appears in Collections:IJEB Vol.61(04) [Apr 2023]

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