Protective effects of grape seed proanthocyanidins on podocytes against injury induced by glucolipotoxicity
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https://doi.org/10.56042/ijeb.v61i04.177Title: Protective effects of grape seed proanthocyanidins on podocytes against injury induced by glucolipotoxicityAuthors:
Li, HaiyanWang, TonglingLiu, DandanDing, YusongLiu, JinbaoKeywords: Diabetic nephropathy;Iron;NF-E2-Related Factor 2;Oxidative stressIssue Date: Apr-2023Abstract: Diabetic nephropathy (DN) is the most common cause of end-stage renal failure, oxidative damage and iron metabolism
disorder are related to DN progression, but the exact pathogenesis remains unclear. Grape seed proanthocyanidins extract
(GSPE) are one of the most widely distributed polyphenols with antioxidant properties. Here, we evaluated the mechanism
by which GSPE alleviates podocyte damage induced by high glucose and palmitic acid (HG/PA). Podocytes were cultured
in groups in normal environment; these were based on whether HG/PA and ML385 were present and the GSPE conc. 10, 25
and 50 mg/L. The flow cytometer, immunofluorescence staining, Western blot, quantitative real time-PCR (qRT-PCR), kit
method and fluorescence microscope were used, respectively. After HG/PA intervention, the podocyte morphology was
destroyed, the survival rate was reduced, the cell apoptosis was increased, and the fluorescence intensity of ROS detection
was increased, iron metabolism was disordered. After adding ML385, increased oxidative damage to podocyte, nuclear
factor erythroid 2-related factor 2 (Nrf2) and its downstream protein and mRNA expression levels were significantly
reduced. However, application of different concentrations of GSPE could reverse these situations. Our findings indicate that
GSPE regulates intracellular iron metabolism disorders and reduces the oxidative damage of podocytes possibly through
activation of the Nrf2 signaling pathway.Page(s): 265-275ISSN: 0975-1009 (Online); 0019-5189 (Print)Appears in Collections:
IJEB Vol.61(04) [Apr 2023]Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.
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