Utilisation of Mangifera indica plant extracts and parts in antimicrobial formulations and as a pharmaceutical excipient: a review

Based on the studies considered, the possible mechanisms of action of M. indica phytochemicals are summarized below.

Complexation of iron (Fe) needed by microbes

All the major phytochemicals, e.g. tannins, flavonoids (quercetin), mangiferin are reported to possess iron chelating property. According to Engels et al. [30], the phytochemical gallotannin possesses strong affinity for iron and readily binds to the metal on microbial cell surface forming a complex. Iron is needed for important metabolic activities by microbes; hence, they secrete biomolecules known as siderophores which aid in sequestration of iron for their use.

Siderophores have high affinity for iron and bind the metal to form ferrisiderophores. Gallotannin is structurally similar to siderophore; hence, it binds the membrane receptor of the microbe in its place. This deprivation of the much needed iron results in inhibition of microbial growth and ultimately cell death. Complexation of iron also results in precipitation of microbial proteins through interaction with cell membrane.

Inhibition of quorum sensing (QS) system in microbes

QS is a gene regulatory system that coordinates genetic expression. QS mechanism involves synthesis, release and uptake of auto-inducers among other processes in microbes [45]. Auto-inducers are extracellular signalling molecules synthesized by microbes for regulation of genes encoding bacterial expressions such as virulence, motility and proteolytic enzymes secretion. Hussain et al. [45] in their study tested different extracts of mango leaf (petroleum ether, benzene, ethyl acetate, acetone and methanol) against C. violaceum strain CV12472 for QS modulatory activity. Pyrogallol was reported as the main phytochemical in M. indica leaf extracts. The study recorded that QS inhibitory ability of the extract was dose dependent. Hussain and colleagues concluded that mango leaf extract possesses the ability to disable the QS system of C. violaceum strain CV12472. According to Asfour [46], QS system inhibition can be effected through:

(i)

Inhibition of synthesis of auto-inducers.

(ii)

Antagonism of auto-inducer receptors

(iii)

Degradation of auto-inducers among others.

Other effects resulting from inhibition of quorum sensing system include:

(a)

Inhibition of biofilm formation

(b)

Neutralization of bacterial toxins

Damage to microbial cell membrane

Raybaudi-Masillis et al. [47] reported that phenols cause damage to microbial cell membrane by interacting with microbial enzymes. The process was reported to involve adsorption of phytochemicals by the cell membrane. This adsorption of phenolic compounds causes alteration of pH and electrical potential of the microbial cell membrane. Damage to microbial cell membrane leads to (1) leakage of cytoplasmic material and (2) cell death.

Disruption of microbial enzyme synthesis

Phytochemicals also exhibit their antimicrobial ability via inhibitory activities on microbial enzyme synthesis. Bodiba et al. [48] conducted a research on the mechanism of action of mango leaf ethanol extract on S. mutans. They reported that the synthesis of the GTF enzyme encoded for by the gtfß gene was disrupted in the presence of the extract. S. mutans gtfß mRNA degree of expression in the presence of the mango leaf extract was compared to that without the extract using reverse transcription polymerase chain reactions. It was reportedly found to be lower in the presence of the extract.

Interference with the replication cycle of viruses

Another mode of antimicrobial action reportedly demonstrated by phytochemicals is inhibition of viral replication cycle. According to Nijveldt [40], flavonoids inhibit various stages of the replication cycle of target virus via (1) inhibition of the intracellular replication of the virus and (2) inhibition of the infectious properties of the virus. However, the study reported that most studies focused on inhibitory activity of reverse transcriptase or RNA-directed DNA polymerase. In addition, it was reported that the investigations were carried out in vitro and thus, the scope of findings was limited.

Modification of microbial membrane properties

Gallotannin is reputed to possess the ability to form complexes with proteins. The mechanism of this action has been found to be in two stages. First, it binds to microbial protein. Secondly, it causes aggregation of the microbial proteins resulting in precipitation [31]. These effects cause modification of microbial membrane properties and change in microbial cell membrane fluidity.

Antimicrobial formulations from M. indica extracts

Phytochemicals are reputed to confer pharmacological attributes on plants. Several researches have been conducted to validate ethno-pharmacological claims of the medicinal efficacy of mango plant part extracts. M. indica plant part extracts are reported to possess antimicrobial properties. Based on this claim, studies have been conducted to formulate antimicrobial therapeutic preparations from the plant extracts. These formulations include skincare products, nutraceuticals, hygiene products, pharmaceuticals among others.

Mango antiseptics ointments, cream and lotions

Okareh and colleagues [4] formulated antimicrobial ointments from mango kernel, leaf and guava leaf methanol extracts and investigated their antibacterial activities against S. aureus, E. coli and Salmonella sp. The ointments were formulated according to the British Pharmacopoeia procedure. Each plant extract (50 mg) was incorporated into ointment bases, respectively, to produce 5% (w/w) antiseptic ointment preparations. Microbiological assay was conducted using agar dilution and diffusion techniques. The analyses were conducted in duplicates. Gentamicin (10 mg/L) was used as the reference drug while ointment base was used as the negative control. The differences in mean zone of inhibitions (ZOI) among the extracts and among ointment formulations against all organisms of interest in the study were reported to be significant. Mango kernel extract and formulation were found to exhibit highest inhibitory activity. In addition, S. aureus was reported to exhibit the highest susceptibility to the plant extracts and ointment preparations. MIC for M. indica kernel ointment was found to be 25 mg/mL for S. aureus, E. coli and Salmonella sp. In addition, the researchers found that mango kernel extract and ointment exhibited highest significant antibacterial activity (20.70 ± 1.05 and 18.00 ± 0.89, respectively) against S. aureus. It was concluded that M. indica kernel ointment exhibited highest antibacterial efficacy among the ointment formulations. In addition, the kernel ointment was reported to possess antibacterial activity against S. aureus comparable to gentamicin the reference antibiotic.

Ningsih et al. [49] formulated antifungal ointment from mango leaf methanol extract and investigated its antifungal activity against C. albicans using agar diffusion method. Ketoconazole was used in the study as reference drug (positive control) while ointment preparation without extract was the negative control. The result of ZOI obtained at 30, 65 and 125 ppm of ointment formulation was reported to be 3.07 mm, 5.96 mm and 9.51 mm, respectively. The ZOI for positive control was reported to be 14.13 mm. The pH value of 6.56- 6.99 was reported. Another study by Ningsih et al. [50] was conducted to formulate an antibacterial ointment from mango leaf methanol extract. The inhibitory activity of the ointment was assessed against Propionibacterium acnes using the agar diffusion method which was indicated by measurement of the ZOI for 15 days. The pH of the ointment was reported to be 4.92–5.87. The researchers reported that ZOI for day zero at 15 ppm was 23.60 mm and 21.88 mm for day 15. The research concluded that the growth of P. acnes was inhibited by mango leaf extract and formulated ointment.

A study by Rao et al. [51] was conducted to formulate a topical gel from mango leaf methanol extract. 100 mg (F1), 200 mg (F2) and 300 mg (F3) of extract were used in the preparation of the ethosomal topical gel formulations 1, 2 and 3, respectively. Antibacterial activity of the gel formulations was assessed against P. aeruginosa and B. subtilis using agar well diffusion technique. Ciprofloxacin and norfloxacin were used as reference drugs. The formulations were reported to have pH range between of 5.4–6.2. The drug content of gels ranged between 74.67% and 82.31%. The study reported that formulation 2 exhibited highest antibacterial activity among the formulations with ZOI of 9 mm for B. subtilis and 13 mm for P. aeruginosa.

Body scrub, soap and disinfectants

Bahari and colleagues [52] formulated two body scrubs using mango seed flour. Methanol extract was obtained from Perlis Sunshine mango seed flour and used to formulate rough salt and oil-in-water (o/w) scrubs, respectively. One gram (1 g) of extract was incorporated into 100 g of base. Body scrub without the seed flour extract was used as control. HPLC was used to measure ascorbic acid content in the body scrubs. The study found that the oil-in-water mango seed flour scrub possesses highest total phenolic content (6.65 ± 0.16 mg/g) and better antioxidant potential (32.29 ± 2.60% of DPPH activity inhibition) among the body scrubs (rough salt formulation, oil-in-water and control). The rough salt scrub was reported to have highest ascorbic acid content.

Rodriguez-Fernandez and colleagues [53] formulated liquid soap from mango kernel and seed oil extract. The soap was prepared from 3 g of the seed oil and 0.5 M sodium hydroxide solution via saponification. The antimicrobial activity of the formulation was assessed against Candida auris, E. coli, S. aureus and P. aeruginosa via the disc diffusion technique. Commercial cleaning/disinfecting agent containing isopropyl alcohol was used as control. The cleaning efficiency of the extract formulation was determined by image analysis with different surfaces such as glass, metal and wood. The research reported that the extract formulation did not show significant antimicrobial activity to the test organisms. However, the formulation was reported to demonstrate high comparable cleaning efficiency.

Oral hygiene formulations

Dandekar and Winnier [54] conducted a research to assess the antibacterial activity of mouthwashes prepared from mango and neem twigs extracts against Streptococcus mutans. The formulations were prepared from 25% extract concentration, respectively. Chlorhexidine (0.2%) was used as the positive control. Antibacterial activity was determined via the agar diffusion technique. Zone of inhibitions of extracts were reported to be 19 mm for mango extract and 18.5 mm for neem extract at 25% concentration. The study concluded that mango and neem formulations exhibited satisfactory antibacterial activity against S. mutans. In addition, anti-gingival and anti-plaque activities were reported to be comparable with that of chlorhexidine the reference drug. This report is in contrast to findings reported by Bhat et al. [55].

Bhat and colleagues [55] formulated mouthwash from mango leaf extract and evaluated antimicrobial property on growth of Streptococcus spp. in salivary secretion of volunteers. Anti-gingival inflammation and anti-plaque accumulation activities were also evaluated. The susceptibility of test organisms (S. mutans, Streptococcus mitis, and Streptococcus salivarius) in saliva to the mouthwash was compared to that of chlorhexidine, the reference drug. The study reported that both the M. indica leaf mouthwash (2%) and chlorhexidine mouthwash (0.12%) inhibited growth in microbial population in the test samples. Reduction in plaque build-up and improved gingival health were also recorded. Chlorhexidine mouthwash was reported to exhibit significantly higher efficacy in comparison with mango leaf mouthwash. According to the researchers, chlorhexidine causes dental staining and possesses cytotoxic effects. M. indica mouthwash reportedly exhibited no side effects.

Anand et al. [56] conducted a research to assess the possible utilization of M. indica in oral healthcare. Mango plant and guava plant leaves were extracted, respectively, via maceration method. Ethanol was used as the extraction solvent. 2 mg/mL of each plant extract was used to prepare the oral hygiene formulation. In vitro antimicrobial activity of extracts was investigated against Enterococcus faecalis, E. coli, C. albicans, S. mutans and S. aureus. Residin (0.2% chlorhexidine) and povidone-iodine-based mouth rinse were used as positive control and reference drugs. Ethanol was used as negative control. The susceptibility of the test organisms to plant extracts was determined via agar well diffusion technique. The minimum inhibitory and bactericidal/fungicidal concentrations (MBC/MFC) were determined by micro-dilution technique with slight modification. The study reported that E. coli exhibited the highest susceptibility to M. indica leaf extract (ZOI = 20.33 ± 0.57) among the test organisms. Anand and colleagues also reported that mango leaf extract exhibited antibacterial activity higher than chlorhexidine (ZOI = 15.67 ± 0.57).

Sekar and Abdullah [57] formulated herbal toothpaste from mango, lemon and pomegranate peelings’ methanol extracts. Five grams (5 g) of each extract was incorporated into base for the formulation. Antimicrobial activity of the herbal toothpaste (with 100 mg, 250 mg and 500 mg of toothpaste base, respectively) was investigated against P. aeruginosa, E. coli, B. cereus and S. aureus via disc diffusion technique. Ciprofloxacin was used as the reference drug. The study reported that S. aureus showed the highest sensitivity to the formulated toothpaste among the test organisms. The positive control ciprofloxacin exhibited significantly higher antimicrobial activity against P. aeruginosa, E. coli, B. cereus and S. aureus compared to the extract formulation. The study concluded that M. indica peeling extract possesses promising antimicrobial effect against the organisms of interest in the study.

Wound healing formulations

Espinosa-Espinosa et al. [58] researched the effect of methanol extract of M. indica peel on incision wounds in a murine model using 5% dexpanthenol as positive control. According to the researchers, the incision model was used to assess the healing efficiency of the plant extract. The healing efficiency assessment includes observation of wound contraction, tensile strength, scar formation and histological analysis. Antibacterial activity of the extract was investigated against S. epidermis, P. aeruginosa, E. coli and S. aureus. Acute dermal toxicity and anti-inflammatory property of the extract were also investigated.

The study reported that there was no significant difference in wound contraction closure of mango extract-treated incision wound and dexpanthenol-treated incision. The same observation was reported for the analysis of tensile strength of the incisions.

In addition, it was reported that histological assessment of the mango extract and dexpanthenol-treated incisions showed similarity in architecture. Further, the study reported that M. indica extract exhibited antibacterial activity against all test organisms. The highest antibacterial activity was recorded against S. epidermidis. The MIC of extract for S. epidermidis was 2 mg/ml. It was found to be 4 mg/ml for P. aeruginosa, E. coli and S. aureus. ZOI for S. epidermidis was reported to be 13.8 ± 1.9 mm. It was reported that chloramphenicol demonstrated significantly higher antimicrobial activity (ZOI = 21.8 ± 0.4 mm) compared to the extract. No sign of toxicity was reportedly exhibited by the extract.

Other mango-based cosmeceuticals

The therapeutic effect and cosmetic importance of M. indica is brought into synergy in its use in cosmetic formulations such as mango lipstick and mango cream/lotion. Jain et al. [59] formulated hand-lotion and lipstick from oil derived from M. indica seeds. The butter was extracted from mango seeds using n-hexane as solvent. The hand lotion and formulated lipstick were assessed for their organoleptic properties which include pH, melting point, surface anomalies, spreadability and other parameters. Jain and colleagues reported that the lipstick possesses a cosmetically acceptable look with smooth texture and compatible pH while the lotion showed no phase separation. The study concluded that the formulations were comparable to synthetic chemical products. It was reported that the formulations were devoid of side effects usually associated with synthetic preparations.

Poomanee et al. [60] developed M. indica kernel ethanol extract-loaded anti-acne cosmeceutical. Concentration of the extract nanoemulsions was reported to be 2.5% w/w. It was optimized via response surface technology aimed at enhancing stability and skin permeation. Antibacterial activity of extract-loaded nanoemulsions against P. acnes was evaluated using broth micro-dilution technique. The MIC and MBC were reported to be 3.13 mg/ml and 12.60 mg/mL, respectively. The study reported that the formulation possesses excellent stability profile and exhibited satisfactory antibacterial property. Poomanee and colleagues [60] concluded that mango kernel extract nanoemulsions possess potential as delivery systems for anti-acne cosmetic products.

Therapeutic nutraceutical formulations

Studies have shown that various parts of M. indica contain nutraceutical potential reportedly attributable to its possible antimicrobial activity against some food borne pathogens.

Thambi et al. [61] formulated 25 nutritional recipes from M. indica peel powder. Sensory properties of the recipes were assessed using five-point hedonic scale. Three different extracts were prepared from mango peel powder using acetone, aqueous and ethanol as extracting solvent, respectively. Antimicrobial effects of the different powder extracts against E. coli, Shigella spp, Enterobacter spp, Salmonella typhus and Aspergillus niger strain were conducted using the agar-well diffusion method. The study reported that acetone extract showed highest inhibitory effect against all test bacteria (ZOI = 27 mm for E. coli, 15 mm for Salmonella typhus, 13 mm for Shigella spp and 16 mm for Enterobacter spp.). Ethanol and aqueous extracts were reported to exhibit higher antifungal activity against A. niger compared to acetone extract.

The study concluded that M. indica peel powder possesses nutraceutical and therapeutic potentials. According to the study, this potential was demonstrated through its inhibitory effect against test organisms. Thus, mango peel powder has potential utilization for nutritional and therapeutic purposes in food and health industries.

Rawung et al. [62] developed functional cookies from fermented M. indica fruit and Anredera cordifolia (binahong) leaves. Mango is reported to contain high level of nutrients, fibre, minerals macronutrients and micronutrients. This is in addition to possession of bioactive compounds like vitamin C, beta-carotene and phytochemicals. These secondary metabolites are reported to be the basis of its wound healing property [12]. Rawung and colleagues [60] found that the cookies contain high concentration of vitamin C and significant antioxidant activity implying possession of potential wound healing activity. The researchers reported that the formulation has mean ascorbic acid content of 129.76 ± 8.13 mg/100 g and DPPH antioxidant activity of 35.15 ± 4.34. The study concluded that fermented mango and binahong possess great potential as healthy snack cookies for postoperative patients to accelerate wound healing process.

Pérez-Chabela and Hernández-Alcántara [63] investigated mango flour and flours from other edible plant parts. They reported that these flours are valuable economic alternatives for the improvement of nutritional value and functional quality of processed foods. In addition, mango and other flours assessed were found to be good sources of prebiotic fibres. The study concluded that these flours possess potential prebiotic effect, modulatory activity and effect on composition of gastro-intestinal tract micro-biota (Table 2).

Table 2 Summary of selected antimicrobial formulations from mango plant partsExcipients

Excipients are ingredients used in pharmaceutical product preparations. They serve as binder, disintegrant or are used for pH adjustment. There are synthetic, natural or semi-synthetic excipients. The use of plant derived excipients is fast gaining ground and opening up new avenue to solve current drug delivery issues in the pharmaceutical industry. According to Ologunagba et al. [64], natural products possess comparative advantages over synthetic polymers, hence their increasing use as excipients in formulation systems. Natural excipients include natural polymers such as pectin, gums and mucilage. Some sources of plant derived natural polymers for pharmaceutical preparations are mango peel, banana peel, mango gum, dehydrated banana powder, guar gum, gum karaya, starch, ispaghula husk, chitosan, Lepidium sativum mucilage and fenugreek seed mucilage.

Pectin, gums and other plant-derived excipients

Pectins are hydrophilic polysaccharide carbohydrates. They aid in the liberation of active pharmaceutical ingredient (API) in the upper part of the digestive system through enzymatic catabolic reactions when used as an excipient [65]. Natural disintegrants are reported to have a number of advantages over synthetic disintegrants. These advantages include low cost, nontoxicity and biodegradability. Starches, mango pectin, mango gum, guar gum, soy polysaccharide, plantago seed mucilage, locust bean gum, agar and treated agar are examples of natural disintegrants [66,67,68].

Binders are used in pharmaceutical preparation to strengthen inter-particle bond capacity within a formulation and enable cohesion. Pectin is used in pharmaceutical formulations as binding and gelling agent [69]. It is used as a stabilizer in liquid medications and emulsions, and it increases the thickness of some pharmaceutical preparations [70]. The usefulness of pectin for various applications is dependent on its physicochemical characteristics. These include methoxyl content, the degree of esterification and galacturonic acid content. Other characteristics are sugar content, jellying characteristic, total soluble solids, moisture content, pH and colour. Assessment of arsenic concentration, microbial load and lead content is conducted to ascertain the safety of pectin for use [71]. Gums are hydrocolloids containing deliquescent molecules with strong affinity for water to form thick solutions or gels [72]. Polymeric nanocarriers are substances with small dimension and high surface area with ability to enhance permeability and dissolution of enclosed molecules. This ability enhances their use for medical applications such as imaging, diagnostic techniques and other therapeutic applications. According to Spizziri et al. [73], location-specific release of active pharmaceutical ingredient (API) with antimicrobial property spurred interest in the advancement of novel polymeric nanocarriers. Methods of extraction of natural polymers for utilization in pharmaceutical formulations include conventional acidified water-based method, microwave-assisted method, enzyme-assisted method, subcritical-assisted method and ultrasound-assisted method.

M. indica utilization as an excipient

Mango peel pectin has reportedly found use as a good source of high-quality pectin extraction [68, 74]. Chaiwarat et al. [75] conducted a research aimed at formulation of clindamycin hydrochloride loaded de-esterified low-methoxyl M. indica peel pectin film. M. indica peel pectin was obtained via microwave-assisted extraction technique and de-esterified. Titration technique was used to ascertain the degree of esterification. Sodium hydroxide was used as the base in the reaction. The pectin film was prepared using remodelled ionotropic gelation with a solution-casting method. Antibacterial activities of de-esterified pectin (DP), de-esterified pectin containing clindamycin HCl (DPC) and commercial low methoxyl pectin(cLMP) films were evaluated against stock cultures of S. aureus and P. acnes using disc diffusion method. One gram (1 g) of commercial clindamycin HCl in 100 ml of pectin solution (1% w/v film forming solution) was the positive control. Film without clindamycin HCl was used as negative control. Data were expressed as mean ± standard deviation, and significance of result was analysed.

The study reported that the positive control exhibited insignificantly higher antibacterial activity against all test organisms (42.02 ± 0.52 mm for S. aureus and 77.18 ± 1.50 mm for P. acnes) in comparison with the commercial low methoxyl pectin (cLMP) and de-esterified pectin containing clindamycin HCl (DPC) films, respectively. Drug release profile of cLMP and DP was assessed. The study reported that there was no significant difference in the drug delivery profiles between commercial low methoxyl pectin and de-esterified pectin films. Further, the study stated that cLMPC and DPC films demonstrated considerable amount of drug loading content with no significant difference between the two.

Chaiwarat and colleagues [75] concluded that the de-esterified pectin obtained from M. indica peel has potential as a film forming agent for topical antimicrobial medications comparable to commercial low-methoxyl pectin. M. indica peel pectin thus has potential as an alternative to commercial low-methoxyl pectin utilized in preparation of clindamycin topical formulation commonly used for the treatment of skin infections.

Siddiqui and colleagues [65] extracted pectin from M. indica peel and assessed its potential as a binder in ibuprofen tablet formulation. The micrometric and post compression characteristics were examined to ascertain the size and binding property of the pectin. Mango pectin was extracted via the conventional hot water extraction technique. 50 g, 75 g, 100 g and 125 g of pectin were used for different ibuprofen tablet formulations, respectively. The researchers reported dissolution of 100 g/tablet formulation in less than 5 min. In addition, the micrometric property of the formulation was reported to demonstrate good binding and flowing abilities. The study concluded that pectin obtained from M. indica can be used as disintegrating agent in pharmaceutical compositions.

Ahmed and Abass [76] conducted a research to investigate M. indica gum (extracted from the trunk) as a sustain release polymer in glibenclamide tablets matrix. The gum was extracted using microwave heat extraction method. Binding, sustained release, disintegrating properties of the formulation were evaluated. The tablets were formulated by wet granulation technique. According to Ahmed and colleague [76], assessment of physicochemical, organoleptic and other characteristics such as swelling index, Hausner’s ratio of the formulated tablets yielded results which inferred that M. indica gum possesses binding property. The study concluded that tablets formulated from this gum exhibited favourable sustained drug delivery property.

Ologunagba et al. [64] conducted a study to extract and characterize mango seed endosperm gum. The extraction was carried out using the microwave-assisted technique. Phytochemical, pharmacognostic, microbial and proximate analyses were conducted. The researchers reported the following results: Swelling Index (%) 2.27 ± 0.19, Water binding capacity (%) 112.00 ± 0.10, Bulk Density (g/cm3) 0.51 ± 0.01, Tapped Density (g/cm3) 0.63 ± 0.01, Area of Repose (Aº) 29.35° ± 0.02, Compressibility Index (%) 19.10 ± 0.03 and Hausner’s ratio 1.23 ± 0.05. The study reported that the results obtained were favourable and significant. The researchers concluded that mango seed gum possesses promising binding and disintegrant properties and great potential as excipient in pharmaceutical formulations.

Gragasin et al. [71] processed and assessed M. indica peels as source of pectin for utilization in pharmaceutical formulations. Extraction was carried out via conventional hot water method. The study reported that percentage yield of pectin was dependent on mango peel to solvent ratio, temperature, pH and duration of extraction. The researchers noted that dried mango peel yielded higher pectin quantity. Physicochemical analysis was conducted. The pectin obtained was graded according to standard specifications. The reported result stated that mango pectin is odourless, has methoxyl content range between 12.65 and 12.84% and galacturonic acid content range between 92.82 and 98.65%. In addition, level of esterification was reported to range between 76 and 79%. The study reported that the mango pectin obtained conformed to standard USP specifications.

Malviya and Kulkanir [70] extracted and characterised pectin from mango peels. The pectin was obtained via acidified water-based extraction technique using Soxhlet apparatus. Physicochemical, phytochemical, organoleptic (colour, door, taste, fracture and texture) analyses and micrometric properties of the pectin were evaluated. Micrometric analysis included parameters such as particle size, bulk density, tapped density, true density, flow property, bulkiness, swelling index, surface tension and thickness of the mango pectin. Malviya and colleague reported that mango peel pectin exhibited good flow property, satisfactory surface tension and solubility in warm water. Binding quality of the excipient in tablets was reported to be dependent on surface tension and other parameters [70]. The study concluded that pectin obtained from mango peel has potential utilization as excipient in the preparation of oral medications such as capsules and tablets (Table 3).

Table 3 Summary of excipients obtained from mango plant parts M. indica gum exudate

Ali and colleagues [77] reported that phytochemicals found in plants possess antiviral characteristics which can be harnessed in therapeutic management of viral diseases. The researchers reported that these phytochemicals which comprise quercetin, ellagic acid, ursolic acid, caffeic acid, thymol and caffeic acid can target viral protein and potentially inhibit viral replication. M. indica contains quercetin and ellagic acid in addition to other phytochemicals. Malabadi and colleagues [78] reported that mango gum together with gum exudates from other plants such as okra gum, acacia gum, tamarind gum and cashew gum was used as immunity booster and herbal remedy for throat infections, cough and common cold during second wave of COVID-19 pandemic in India. The study reported that the gums were utilized in the preparation of dietary food recipes and consumed as additional nutritional enhancement for Covid-19 patients during therapy. According to Malabadi and colleagues, consumption of the gum therapeutic diet along with regular medication aided quick restoration of health to patients during COVID-19 pandemic in India. This is a possible indication that M. indica gum has the potential for use in pharmaceutical preparations relating to management of viral infections.

Plant gums have found use in pharmaceutical industry for thickening and stabilizing formulations as binding, suspending and emulsifying agents and for sustained delivery of drugs [72, 79,80,81]. This is because natural gums have been reportedly found to possess advantages such as bio-degradability, lesser toxicity, lower cost of exploitation, abundance, flexibility and biocompatibility over synthetic excipients by researchers.

According to Kaddam et al. [82], although plant gums and mucilage have been reported to possess possible use in therapeutic formulations due to the favourable properties they possess, ingestion of high concentration is potentially toxic. Kaddam and colleagues noted that there are no significant reports of clinical evidence to support inhibition of SARS-CoV-2 viral replication load in patients by natural gums and mucilage (Tables 4, 5).

Table 4 Ethnomedicinal use, validation, antimicrobial activity and main phytochemical constituent of selected M. indica partsTable 5 Antimicrobial activity, functional group and basic nucleus/structure of some selected phytochemicals in mango

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