Rapid and sensitive detection of hazardous compounds are of great importance for sensors. This work presented the immobilized laccase on Fe3O4@MF-CNTs nanocomposites (Fe3O4@MF-CNTs-lac) for the fast and sensitive detection of catechol. Electrochemical impedance spectroscopy (EIS) results revealed that the combined effect of Fe3O4@MF and carbon nanotubes (CNTs) facilitates electron transfer between laccase and electrode. Amperometric curves indicated that the Fe3O4@MF-CNTs-Lac biosensor can respond rapidly to substrate and detect catechol within 0.5 s. The Fe3O4@MF-CNTs-Lac biosensor possesses low detection limit of 0.84 μM (S/N=3) with a linear range of 1-3000 μM, as well as high sensitivity, remarkable organic compounds tolerance, and good selectivity, reproducibility and stability. The catechol biosensing assays revealed that the Fe3O4@MF-CNTs-Lac operates in acidic water environments. Most importantly, the Fe3O4@MF-CNTs-Lac biosensor exhibited reliable results in real water sample analysis. The outstanding biosensing performances reveal that the Fe3O4@MF-CNTs-Lac biosensor has a technical application in the treatment of catechol pollution.

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