Available online 29 March 2023, 115947

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We measured antibiotic penetration and bioavailability in staphylococcus biofilms using simulated humanized concentrations of fluorescent vancomycin plus or minus rifampin. Vancomycin percent recovery across biofilm layers was:upper=46%, middle=40%, and lower=33%. Vancomycin plus rifampin was not significantly different (p= 0.65). Addition of rifampin did not improve vancomycin penetration across biofilm layers.

Section snippetsFunding

This work was unfunded, and the authors received no outside support for this work.

CRediT authorship contribution statement

Kathryn E. Daffinee: Data curation, Writing – original draft, Formal analysis, Investigation, Visualization, Methodology, Project administration. Emily T. O'Neill: Data curation, Writing – original draft, Formal analysis, Investigation, Visualization. Callan R. Bleick: Writing – original draft. Geoff Williams: Data curation, Methodology. Valentin Antoci: Writing – review & editing. Dioscaris Garcia: Writing – review & editing, Validation. Kerry L. LaPlante: Conceptualization, Methodology,

Declaration of Competing Interest

Kerry LaPlante receives research funding from Merck, Pfizer Pharmaceuticals, Shionogi, Paratek, and Entasis. Callan A. Bleick, Emily T. O'Neill, Geoff Williams, Valentin Antoci, and Kathryn E. Daffinee have no relevant financial relationships. Dioscaris Garcia holds equity in BI Medical, LLC.

Acknowledgements

The information provided in this note are those of the authors and do not necessarily reflect the position or policy of the United States Department of Veterans Affairs. This work was supported in part, by the Office of Academic Affiliations (OAA) at the Department of Veterans Affairs.

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