Profiling of circRNA expressions in radiation-treated head and neck cancer cells and the potential role of circPVT1

Elsevier

Available online 28 March 2023, 105690

Archives of Oral BiologyAuthor links open overlay panel, , , , , , ABSTRACTObjective

Radiotherapy is an indispensable treatment modality for head and neck cancers (HNCs). Due to their stable structure, circular RNAs (circRNA) have been implicated as potential biomarkers for clinical use in cancers. The purpose of this study was profiling the circRNA in radiation-treated head and neck cancer cells to identify potential differentially expressed circRNAs.

Design

The effects of radiation on the expression level of circRNAs were investigated in HNCs cells, compared to healthy cell lines. To predict the potential role of circRNAs in HNC patients, tissue expression levels, survival analyses of circRNAs, and circRNA-miRNA network were evaluated using TCGA/CPTAC datasets. Based on expression level in irradiated cells, circPVT1 (plasmacytoma variant translocation 1) was further investigated by sequence analysis.

Results

The study revealed the characterization of differentially expressed circRNAs in cancer cells and that irradiation made significant changes in the expression of circRNAs. These findings suggest that certain circRNAs, especially circPVT1, may be potential biomarkers to monitor radiotherapy effects in patients with HNCs.

Conclusions

CircRNAs may be promising molecules for improving and understanding radiotherapy efficacy in HNCs.

Section snippetsINTRODUCTION

Head and neck cancers (HNCs) are the 6th most common cancer type in the world (Siegel et al., 2016), and squamous cell carcinoma accounts for approximately 90% of them (Pai & Westra, 2009). HNCs show an annual incidence of more than 600,000 new cases worldwide and only a third of these patients are diagnosed in the early stages of HNCs. The 5-year survival rate is approximately 50% for all stages of HNCs, indicating an unfavorable prognosis (Mireștean et al., 2022, Siegel et al., 2016). Here,

Cell Culture

The human pharyngeal epithelial cancer cell line Detroit-562 (ATCC, CCL-138) and human lung fibroblast normal cell line WI-38 (ATCC, CCL-75), were very kindly provided by Prof. Dr. Nalbantsoy (Ege University, Engineering Faculty Department of Bioengineering, Izmir, Turkey). Before using, cells were checked for mycoplasma contamination with a mycoplasma PCR Detection Kit (Sigma Aldrich, USA). Cells were cultured in RPMI-1640 with L-Glutamine (Biological Industries (BI), USA) containing %10 fetal

CircRNAs expressed in head and neck squamous cell line

To evaluate the effect of radiotherapy on circRNAs, we investigated 122 circRNA genes based on literature. According to the result of the survival fraction, Detroit-562 cells were treated with 4 Gy of irradiation (Fig. 1). We subsequently investigate the alterations in circRNA expression profiles of Detroit-562 cells and irradiated Detroit-562 cells, compared to healthy cell control. Among them, 62 different circRNA genes are expressed in at least one of the test groups, including irradiated

DISCUSSION

In this study, the differentially expressed circRNAs due to HNCs cells radiation were investigated. These findings suggest that certain circRNAs expressed in HNCs were decreased after irradiation, also suggesting that their expression levels converged to the healthy cell line. Among them, circPVT1 may be potential biomarker to monitor the effects of radiotherapy in patients with HNC.

In recent years, advanced high-throughput sequencing and computational approaches have identified circular RNA as

CONCLUSION

Due to its abundant presence in tissues and fluids, tissue-specificity, and stable structure, circRNA can show potential diagnostic biomarker capacity for HNCs as well as other cancer types. In addition, circRNAs have attracted attention in the field of radiotherapy, especially due to their stable structure and modulation of radiosensitivity in various tumors. The current study revealed differentially expressed circRNAs after irradiation compared to healthy cells. These findings suggest that

Uncited references

(Li et al., 2018, Wang et al., 2022, Zhou et al., 2021)

FUNDING

This work is supported by EGE UNIVERSITY, Scientific Research Projects with Project No: TYL-2021-22939

ACKNOWLEDGMENT

We thank to Can Holyavkin and Gen Era Diagnostics

CONFLICTS OF INTEREST

The authors have declared no conflict of interest.

CREDİT AUTHORSHİP CONTRİBUTİON STATEMENT

Can Muftuoglu – (1) The conception and design of the study, analysis and interpretation of data, (2) drafting the article, (3) final approval of the version to be submitted. Ufuk Mert – (1) The conception and design of the study, (2) revising it critically for important intellectual content, (3) final approval of the version to be submitted. Ozlem Ozkaya Akagunduz - (1) Acquisition of data, (2)

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