Purpose: Two-point discrimination (2PD) is the ability to discern that two nearby objects touching the skin are truly two distinct points, not one. It is often tested with two sharp points during a neurological examination and is assumed to reflect how finely innervated an area of skin is. objective was to find out the normal value of Two Point Discrimination on the Back, Finger, Forehead & Sole and to Correlate values of Two Point Discrimination on Back, Finger, Forehead & Sole with corresponding BMI according to Gender. Methods: Two point discrimination test is a part of practical course of Physiology Department of the teaching institute. Here, the students perform the practical under strict supervision of professors, the data of the students practicals is recorded for department personal use. This data was collected with the permission from the head of the department and then analyzed to see the normal values of two-point discrimination and also to assess the correlation between Gender, BMI, & Two-point Discrimination (TPD). Pearson Correlation, T-test, SEM, CI95, Scatter diagrams were used to analyze and understand the data. Results: Values of Two-Point-Discrimination(TPD) Average(in mm), SD, and Pearson Correlation (with BMI) at Back (Males=32.19 +/- 4.94,r=0.808 & Females=29.38 +/-5.29,r=0.848), Fingertips (Males=3.43 +/- 1.52,r=0.0344 & Females=3.47 +/- 1.52,r=0.0141), Forehead (Males=13.53 +/- 2.36,r=0.0223 & Females=12.25 +/- 2.61,r=0.198), Sole (Males=14.69 +/- 3.54,r=0.00493 & Females=13.87 +/- 3.36,r=0.0234). Conclusion: The results depict the normal values of Two-point discrimination at the respective location.The normal values at a particular location in Males and Females are almost the same. Strong Correlation of TPD value at Back with BMI can be justified as fat Accumulation increases in Skin with increasing BMI. Fat mainly accumulates on the abdomen, thighs, and back and neck but not on Forehead, Fingertips & soles. Fat accumulation hypothetically leads to increase skin surface leading to virtually variable per area relative decreased skin innervation resulting in higher TPD values and Strong Positive Correlation. TPD values at Forehead, Fingertips & soles do not correlate with BMI.

The authors have declared no competing interest.

This study did not receive any funding

I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained.

Yes

The details of the IRB/oversight body that provided approval or exemption for the research described are given below:

IRB os Smt. NHLMMC (Shrimati Nathiba Hargovindas Lakhamichand Municipal Medical College) gave Ethical approval for this work

I confirm that all necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived, and that any patient/participant/sample identifiers included were not known to anyone (e.g., hospital staff, patients or participants themselves) outside the research group so cannot be used to identify individuals.

Yes

I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance).

Yes

I have followed all appropriate research reporting guidelines, such as any relevant EQUATOR Network research reporting checklist(s) and other pertinent material, if applicable.

Yes

All data produced in the present study are available upon reasonable request to the authors