Solitalea lacus sp. nov., isolated from pond sediment

Abstract

A Gram-stain-negative, strictly aerobic, oxidase-positive, catalase-negative, motile by gliding, creamy white-pigmented bacterium, designated strain S2-8T, isolated from a sediment sample from a Wiyang pond in the Republic of Korea, was subjected to polyphasic taxonomic analysis. Growth was observed at 10–40 °C (optimum: 30 °C), pH 7–8 and 0–0.5% NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain S2-8T belonged to the family Sphingobacteriaceae in the phylum Bacteroidota and was closely related to Solitalea longa HR-AVT, Solitalea canadensis DSM 3403T and Solitalea koreensis R2A36-4T with 97.2, 96.7 and 93.7 % 16S rRNA gene sequence similarities, respectively. Average nucleotide identity and digital DNA–DNA hybridization values for these type strains were 72.0–75.2% and 21.2–21.9 %, respectively. The major respiratory quinone is menaquinone-7. The major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (comprising C16 : 1  ω7c and/or C16 : 1  ω6c). The major polar lipids were phosphatidylethanolamine, two unidentified amino acids and four unidentified lipids. The G+C content of genomic DNA was 37.9 mol%. Based on polyphasic taxonomic analysis, it was observed that strain S2-8T is a novel species belonging to the genus Solitalea , for which the name Solitalea lacus sp. nov. is proposed. The type strain is S2-8T (= KACC 22266T= JCM 34533T).

Received: 23/02/2022 Accepted: 28/11/2022 Published Online: 20/03/2023

Funding

This study was supported by the: Nakdonggang National Institute of Biological Resources (Award NNIBR202201104) Principle Award Recipient: NotApplicable

© 2023 The Authors

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/content/journal/ijsem/10.1099/ijsem.0.005743

2023-03-20

2023-03-21

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