Eliminating OFF-frame clones in randomized gene libraries: An improved split β-lactamase enrichment system
List of abbreviationsAlBla-7B6-OmBla
gene block with alpha beta-lactamase, 7B6 clone and omega beta-lactamase
CDR
complementary determining region
CFU/mL
colony forming units per milliliter
IGV
Integrated Genomics Viewer
IN-frame
clones where translation is in frame
LB broth
Luria Broth broth
OFF-frame
clones where translation is out of frame
ONT
Oxford Nanopore Technologies
pTP_AlBla-7B6-OmBla
A plasmid with the gene-block AlBla-7B6-OmBla
pTP_AlBla-7B6-OmBla_OFF
A plasmid with the gene-block AlBla-7B6-OmBla, with a deletion
pTP_Lib
TP1122 plasmid with ssDNA library oligos cloned
pTP1122
plasmid used as template for curation system
scFv
Single chain antibody fragment
sdAb
Single domain antibody
SDM
Site-directed mutagenesis
SOE-PCR
Splice-overlapping PCR
TRIM
Trimer phosphoramidites
Keywords
Randomized gene libraries
Split beta-lactamase
Functional diversity
Curation system
Frameshifts
Antibody libraries
Data availability
Data is available at https://figshare.com/s/db050fa0b81592a51755 and has an official doi reserved which will be active after publication.
ONT sequencing data (Original data) (Figshare)
© 2023 The Authors. Published by Elsevier B.V.
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