The role and clinical significance of microRNA-29a-3p in the development of hypopharyngeal carcinoma

Brazilian Journal of Otorhinolaryngology

Available online 10 March 2023

Brazilian Journal of OtorhinolaryngologyAuthor links open overlay panel, , , , , , , , Highlights•

The results showed that microRNA-29a-3p expression was relatively high in non-cancer tissue cells but was relatively low in cancer tissue.

Tumor size, T-stage, tumor differentiation, postoperative recurrent-metastasis, the expression of Ki67 and E-cadherin, and the survival time of hypopharyngeal cancer patients were significantly negatively correlated with microRNA-29a-3p.

This study found the relationship between miRNA-29a-3p and the occurrence and development of HPC.

This study will contribute to the individualized treatment of HPC, which improve the treatment strategy and prognosis evaluation system of HPC.

AbstractObjective

MicroRNA-29a-3p has been reported in a variety of cancers, but its role in hypopharyngeal cancer remains unclear. This study was to determine the role of microRNA-29a-3p in the occurrence and development of hypopharyngeal cancer.

Methods

40 patients with hypopharyngeal cancer who underwent surgery in the Affiliated Hospital of Jining Medical University from April 2013 to November 2017 were selected for this study. The cancer tissue samples of the patients were collected, and the patients were followed up for three years. The expression of microRNA-29a-3p in tissue samples was detected by in situ hybridization with fluorescent probe, and the relationships among microRNA-29a-3p and clinicopathological factors, postoperative recurrent-metastasis, survival time were studied. Immunohistochemical was used to detect the expression of Ki67 and E-cadherin in tissue samples.

Results

Combined with HE staining results showed that microRNA-29a-3p expression was relatively high in non-cancer tissue cells (red blood cells and fibroblasts in tumor interstitial vessels), but was relatively low in cancer tissue and cells. According to the follow-up data of 40 patients with hypopharyngeal cancer, tumor size, T-stage, tumor differentiation, postoperative recurrent-metastasis of hypopharyngeal cancer patients were significantly negatively correlated with microRNA-29a-3p (p < 0.05). Immunohistochemica results further confirmed that microRNA-29a-3p was negatively correlated with the expression of Ki67 and E-cadherin. The survival time of patients positively related with microRNA-29a-3p expression (p < 0.05). Moreover, ROC curve analysis showed that the area under the curve of the combined detection of miRNA-29a-3p+Ki67+E-cadherin was larger than that of the single detection of the three indexes.

Conclusions

The expression of microRNA-29a-3p is closely related to the occurrence, development and prognosis of hypopharyngeal cancer, and it affects the proliferation and invasion. This indicates that microRNA-29a-3p serves as a therapeutic target for the occurrence and development of hypopharyngeal cancer.

The evidence of study designs of this study is IV using “Oxford Centre for Evidence-Based Medicine 2011 Levels of Evidence”.

Keywords

Hypopharyngeal cancer

MicroRNA-29a-3p

Ki67

E-cadherin

Survival time

© 2023 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Published by Elsevier España, S.L.U.

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