Artificial intelligence in clinical multiparameter flow cytometry and mass cytometry – key tools and progress

Elsevier

Available online 5 March 2023

Seminars in Diagnostic PathologyAuthor links open overlay panel, , , Abstract

There are many research studies and emerging tools using artificial intelligence (AI) and machine learning to augment flow and mass cytometry workflows. Emerging AI tools can quickly identify common cell populations with continuous improvement of accuracy, uncover patterns in high-dimensional cytometric data that are undetectable by human analysis, facilitate the discovery of cell subpopulations, perform semi-automated immune cell profiling, and demonstrate potential to automate aspects of clinical multiparameter flow cytometric (MFC) diagnostic workflow. Utilizing AI in the analysis of cytometry samples can reduce subjective variability and assist in breakthroughs in understanding diseases.

Here we review the diverse types of AI that are being applied to clinical cytometry data and how AI is driving advances in data analysis to improve diagnostic sensitivity and accuracy. We review supervised and unsupervised clustering algorithms for cell population identification, various dimensionality reduction techniques, and their utilities in visualization and machine learning pipelines, and supervised learning approaches for classifying entire cytometry samples.Understanding the AI landscape will enable pathologists to better utilize open source and commercially available tools, plan exploratory research projects to characterize diseases, and work with machine learning and data scientists to implement clinical data analysis pipelines.

Section snippetsBackground

The World Health Organization's classification of hematopoietic and lymphoid tissue recognizes lineage assignment by immunophenotyping as essential in establishing and subcategorizing hematolymphoid malignancies. Multiparameter flow cytometry (MFC) is a major contributor to establishing immunophenotypes of cell populations. Traditionally, processing and staining of samples for flow cytometry and analysis of flow cytometric data have been labor-intensive processes that require highly trained

Companies using machine learning for clinical cytometry

As we continue to delve deeper into the nuances of the immune system, increasing computational power, dataset sizes, and advanced tuned reagents and instruments can help with disease insights, precision medicine, drug discovery, and disease monitoring. Below are four biotechnology startups that are driving forward the capabilities in clinical cytometry research studies and routine immune profiling and monitoring.

Discussion

For MFC and mass cytometry, the number of supervised approaches has historically lagged unsupervised approaches but is beginning to see an uptick especially at the sample level. Cheung et al. provided a detailed account in 2020 of supervised and unsupervised algorithms for MFC data analysis, including if the algorithms were implemented inside of a GUI, and whether they were accessible for free or through a paid platform95. While unsupervised approaches can be conceptualized and immediately

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