Single-cell RNA-Seq reveals transcriptional regulatory networks directing the development of mouse maxillary prominence

Elsevier

Available online 24 February 2023

Journal of Genetics and GenomicsAuthor links open overlay panel, , , , , , Abstract

During vertebrate embryonic development, neural crest-derived ectomesenchyme within the maxillary prominences undergoes precisely coordinated proliferation and differentiation to give rise to diverse craniofacial structures, such as tooth and palate. However, the transcriptional regulatory networks underpinning such an intricate process have not been fully elucidated. Here, we perform single-cell RNA-Seq (scRNA-Seq) to comprehensively characterize the transcriptional dynamics during mouse maxillary development from embryonic day (E) 10.5 to 14.5. Our single-cell transcriptome atlas of ∼28,000 cells uncovers mesenchymal cell populations representing distinct differentiating states and reveals their developmental trajectory, suggesting that the segregation of dental from the palatal mesenchyme occurs at E11.5. Moreover, we identify a series of key transcription factors (TFs) associated with mesenchymal fate transitions and deduce the gene regulatory networks directed by these TFs. Collectively, our study provides important resources and insights for achieving a systems-level understanding of craniofacial morphogenesis and abnormality.

Keywords

Craniofacial development

Single-cell RNA-Seq

Maxillary prominences

Gene regulatory network

Transcription factor

© 2023 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, and Genetics Society of China. Published by Elsevier Limited and Science Press.

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