Advances in Pharmacology and Pharmacy Vol. 11(2), pp. 156 - 167
DOI: 10.13189/app.2023.110207
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Abdullah A. A. Alghamdi 1, Nada M. Ali 2, Mohammad Mahboob Alam 2, Syed Nazreen 2, Ali Mahzari 3,*
1 Department of Biology, Faculty of Science, Al Baha University, Al Baha, Saudi Arabia
2 Department of Chemistry, Faculty of Science, Al Baha University, Al Baha, Saudi Arabia
3 Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Al Baha University, Al Baha, Saudi Arabia

ABSTRACT

Antioxidants, in addition to protection against oxidative stress, also possess anti-inflammatory and anticancer potentials. In this work, the ethanolic extract (EE) of Euphorbia inarticulata has been evaluated for antioxidant, anti-inflammatory and anticancer activities. The antioxidant activity showed that EE significantly scavenges DPPH (IC50 = 54.56 ± 2.38 µg/ml) and H2O2(77.44 ± 2.76 µg/ml), whereas ferric reducing antioxidant power (FRAP) and total antioxidant capacity (TAC) were found to be 100.93 ± 4.15 µg/ml and 46.72 ± 3.86 mg gallic acid equi/gm, respectively. The EE exhibited significant inhibition on COX-1 (IC50 79.84µg/ml) and protein denaturation (IC5057.6 µg/ml), compared to indomethacin and diclofenac, respectively. Furthermore, EE was found to be effective in killing Huh-7 and HeLa cancerous cells with IC50 104.62 (SI 2.36) and 145.11 µg/ml (SI 1.7), respectively, while doxorubicin displayed IC50 0.71 (Huh-7) and 2.63 µg/ml (HeLa), whereas it was safe on normal MRC-5 cells. The HPLC analysis showed the presence of ellagic acid, caffeic acid, gallic acid, syringic acid, cinnamic acid and catechol which may be responsible for the promising antioxidant, anti-inflammatory and anticancer potentials exerted by the ethanolic extract of E. inarticulata. These results conclude that Euphorbia inarticulata ethanolic extract has anti-inflammatory and anticancer effects by triggering oxidative stress.

KEYWORDS
Euphorbia inarticulata, Antioxidant, Anti-inflammatory, Anticancer, HPLC

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Abdullah A. A. Alghamdi , Nada M. Ali , Mohammad Mahboob Alam , Syed Nazreen , Ali Mahzari , "HPLC Profile, Anticancer, Anti-inflammatory and Antioxidant Activities of Euphorbia inarticulata Ethanolic Extract," Advances in Pharmacology and Pharmacy, Vol. 11, No. 2, pp. 156 - 167, 2023. DOI: 10.13189/app.2023.110207.

(b). APA Format:
Abdullah A. A. Alghamdi , Nada M. Ali , Mohammad Mahboob Alam , Syed Nazreen , Ali Mahzari (2023). HPLC Profile, Anticancer, Anti-inflammatory and Antioxidant Activities of Euphorbia inarticulata Ethanolic Extract. Advances in Pharmacology and Pharmacy, 11(2), 156 - 167. DOI: 10.13189/app.2023.110207.