Antifungal activity of the ethanolic extract and flavonoid avicularin from Myrcia tomentosa (Aubl.) DC. on virulence factors of Candida species

In this work, the mechanism of the action of the ethanolic extract and avicularin isolated from Myrcia tomentosa was evaluated against Candida albicans and Candida parapsilosis. The effects of ethanolic extract and avicularin regarding adhesion in epithelial cells and on yeast kinetic growth were tested. A neutral red uptake (NRU) assay was used to test cytotoxicity against the 3T3-A31 cells of Balb/c. The morphology and ultrastructural analysis were examined by scanning and transmission electron microscopy. The results showed good activity for the ethanolic extract and the flavonoid avicularin, which inhibited the yeasts in concentrations of 32 and 16 µg/mL, respectively. The plant samples promoted inhibition of the adhesion in epithelial cells and showed a reduction in the number of the yeasts in kinetic growth. Avicularin and ethanolic extract from M. tomentosa showed low in vitro toxicity for the 3T3-A31 cells. The scanning and transmission electron microscopy showed changes in morphology and cell arrangement of Candida species in the presence of avicularin. Considering the results, avicularin obtained from M. tomentosa demonstrates potential for the future treatment of candidiasis.

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