Clinical Investigations – Research Article

Mohamad N.a· Khedr A.M.B.a· Shaker O.G.b· Hassan M.a

Author affiliations

aDepartment of Dermatology, Faculty of Medicine, Fayoum University, Faiyum, Egypt
bDepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Giza, Egypt

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Article / Publication Details

First-Page Preview

Received: April 08, 2022
Accepted: October 26, 2022
Published online: February 02, 2023

Number of Print Pages: 10
Number of Figures: 4
Number of Tables: 5

ISSN: 2296-9195 (Print)
eISSN: 2296-9160 (Online)

For additional information: https://www.karger.com/SAD

Abstract

Introduction: Alopecia areata (AA) is a common autoimmune condition that affects anagen hair follicles. The most commonly recognized theory is that it is a T-cell-mediated autoimmune disorder in a genetically susceptible individual. MicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs) were thought to play a function in the pathogenesis. The expression of lncRNA HOTAIR and miRNA-205 and their relation to transforming growth factor β1 (TGF-β1) in AA were not studied. Aim: The aim of the studywas to evaluate the role of miRNA-205, lncRNA, HOTAIR, and TGF-β1 levels in AA pathogenesis, clinical course, and severity of AA. Methods: Two groups of subjects were included in this case-control study: 50 patients with AA and 50 healthy matched controls. miRNA-205 and lncRNA HOTAIR expression levels were assayed using quantitative RT-PCR, while serum levels of TGF-β1 were assayed using ELISA techniques. Results: The serum expression of lncRNA HOTAIR was significantly downregulated in AA patients with a p value < 0.001, while the serum expression of both miRNA-205 and TGF-β1 were significantly upregulated in patients. Discussion/Conclusion: This study highlights the potential role of high serum expression of miRNA-205 and TGF-β1 and the low serum expression of lncRNA HOTAIR in AA pathogenesis. This could be used as a therapeutic target to treat AA.

© 2023 S. Karger AG, Basel

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First-Page Preview

Received: April 08, 2022
Accepted: October 26, 2022
Published online: February 02, 2023

Number of Print Pages: 10
Number of Figures: 4
Number of Tables: 5

ISSN: 2296-9195 (Print)
eISSN: 2296-9160 (Online)

For additional information: https://www.karger.com/SAD

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