Dummy-template Pickering emulsion imprinted microspheres online pretreatment and analysis for the estrogens in cosmetics

ElsevierVolume 1691, 22 February 2023, 463815Journal of Chromatography AAuthor links open overlay panelHighlights•

A novel on-line SPE-HPLC analytical system was constructed.

Pickering emulsion polymerization was used to obtain monodisperse microspheres.

The dummy-template technology was used.

Abstract

Estrogens are a class of steroid hormone with strong physiological activity. Due to the pronounced beauty effect, such drugs are highly susceptible to illegal addition and cause other adverse effects. To avoid template leakage and the negative impacts on the environment caused by the estrogens, diosgenin was selected as the dummy template due to its similar skeleton structure. The Pickering emulsion polymerization was used to obtain the dummy-template molecularly imprinted polymers (dt-MIPs). Scanning electron microscopy, optical microscopy, specific surface area testing, Fourier transform infrared spectroscopy and adsorption experiments were used to characterize the apparent morphology and the recognition performance of the microspheres. Then, the prepared microspheres and commercial fillers were used to construct an on-line solid phase extraction (on-line SPE) analytical system coupled with HPLC via a two-position switching valve. On-line solid phase extraction-HPLC analytical methods were established and verified, for the simultaneous determination of four estrogens in cosmetic samples. The accuracy and precision RSDs for the established methods using the imprinted sorbents were 92.00–104.02% and less than 9.12%, respectively. All four estrogens exhibited good linearity in the range of 0.05 to 5 µg/mL with a coefficient of determination R2 greater than 0.9810. The method comparison results suggest that the established analytical method is simple in pre-treatment, easy to automate, and has excellent sensitivity to meet the analytical requirements of complex samples.

Keyword

Dummy template

Molecular imprinted polymers

Pickering emulsion

Online solid phase extraction

Estrogens

Data availability

Data will be made available on request.

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