Potential of a methanolic extract of Lawsonia inermis (L.) leaf as an alternative sanitiser in the time of COVID-19 Pandemic and beyond

Elsevier

Available online 25 January 2023, 100633

Journal of Herbal MedicineAuthor links open overlay panelHighlights•

Bioactivity of methanolic extract of Henna leaf was investigated in 4 solutions

The antibacterial activity was in the increasing order of AH > dH2O >>>> TBH > PBS

Complete cessation of the antiviral activity observed in TBH and PBS

However, the antiviral activity was retained and enhanced in AH and dH2O

The extract has potentials as an alternative sanitiser

Abstract

To harness the antimicrobial properties of a crude methanolic extract of Henna (Lawsonia inermis) leaf as a potential alternative sanitiser, there is the need to test its performance in different solutions. In this work, the effects of distilled water (dH20), Acetate-HCL (AH) Buffer (pH 4.6), Phosphate Buffer Saline (PBS) (pH 7.2) and Tris-HCL (TBH) Buffer (pH 8.6) on the antibacterial and antiviral activity of the extract were assessed. Through standard phytochemical screening and HPLC-MS (LCMS STANDARD 7.M), it was found that the extract consisted of about 30 different compounds including flavonoids. The extent of the antimicrobial activity of the extract in solutions was in the increasing order of AH > dH2O >>>> TBH > PBS. Under the same conditions, reduced antibacterial activity and complete cessation of the antiviral activity of the extract in TBH and PBS was observed. However, in AH and dH20, within 1 to 5 minutes, 1 mg ml-1, 0.125 mg ml-1 and 0.0625 mg ml-1 of the extract caused complete inactivation of E.coli (reductions of 8.2 log CFU ml-1), B. subtilis (reductions of 8.2 log CFU ml-1) and MS2 (reductions of 9.7 log PFU ml-1) respectively. The fluorescence microscopy images of the live/dead staining of the inactivated bacterial samples validated the extent of the inactivation. The broad spectrum and high antimicrobial activity of the extract, coupled with the plant not a staple food, has long history of safe use by humans as a medicine and cosmetic, cheaply available in abundance in many regions of the world, thus making the extract a potential candidate as an alternative sanitiser in the time of COVID-19 Pandemic and beyond.

AbbreviationsPBS

Phosphate Buffer Saline

LM

methanolic extract of Lawsonia inermis leaf

Keywords

Lawsonia inermis

Sanitiser

methanolic extract

antibacterial

antiviral

Henna

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