Abstract

The cultural parameters of Streptomyces sp. for pectinase production were optimized using the Box-Behnken design. The maximum pectinase production was obtained after 58 h at 35°C and pH 7 upon submerged fermentation in yeast extract-containing media. The enzymes were partially purified with acetone precipitation, and the analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and zymogram revealed that Streptomyces sp. produced two pectinases protein with molecular weights of about 25 and 75 kDa. The pectinase activity was detected in a wide range of temperatures (30°C–80°C) and pH (3–9) with maximum pectinase activities observed at 70°C and pH 5 and 9. The enzymes retained about 30–40% of their activities even after incubating the enzyme at different temperatures for 120 min. The pectinase activities of Streptomyces sp. were enhanced in the media containing 1.5% pectin, 1% casein as a nitrogen source, 0.5 mM MgSO4, and 5 mM NaCl. Further, the addition of Tween-20, amino acids, and vitamins to the media also enhanced the pectinase activity. Moreover, the bacterium illustrated the ability to decolorize crystal violet dye efficiently. The decolorization rate ranged from 39.29 to 53.75%, showing the highest bacterial decolorization in the media containing 2 mg/mL crystal violet at 144 h. Therefore, the bacterium has the potential in treating wastewater produced by industries like textile industries.

© 2022 The Author(s). Published by S. Karger AG, Basel

References _Journal>Int J Curr Microbiol Appl Sci. 2016;5:514–21. _Journal>Sustain Environ Res. 2021;31(1):8. _Journal>Process Biochem. 2009;44(5):521–6. _Journal>Appl Microbiol Biotechnol. 2002;59(4-5):409–18. _Journal>Sci Rep. 2021;11:9754–24. _Journal>Process Biochem. 2005;40(9):2931–44. _Journal>Bioresour Technol. 2001;77(3):215–27. _Journal>Indo Am J Pharm Res. 2016;6:4185–90. _Journal>Biosci Biotechnol Res Commun. 2018;11(1):18–30. _Journal>Annu Rev Food Sci Technol. 2013;4:21–34. _Journal>Biocatal Agric Biotechnol. 2015;4(3):279–85. _Journal>J Pharm Chem Biol Sci. 2017;6:23–34. _Journal>World J Microbiol Biotechnol. 2004;20(3):257–63. _Journal>Food Biotechnol. 2012;26(2):107–23. _Journal>Anal Chem. 1959;31(3):426–8. _Journal>Biocatal Agric Biotechnol. 2015;4(1):70–6. _Journal>Int J Biol Macromol. 2016;82:127–33. _Journal>PeerJ. 2018a2018;6:e5015. _Journal>Enzyme Res. 2018b2018;2018:3859752. _Journal>World J Microbiol Biotechnol. 2001;17(5):487–91. _Journal>Fermentation. 2021a;7(1):40. _Journal>Appl Microbiol Biotechnol. 2021b;105(24):9069–87. _Journal>Enzyme Res. 20122012;2012:138634. _Journal>Appl Microbiol Biotechnol. 1993;39(1):36–41. _Journal>Int J Food Prop. 2016;19(11):2443–50. _Journal>Bioresour Technol. 2019;272:26–33. _Journal>Molecules. 2013;18(11):14366–80. . Appl Biochem Biotechnol. 2015;176(3):758–71. + inhibition into pectinase activities. Jurnal Penelitian Pengkajian Pendidikan E-Saintika. 2020;4(2):112. isolated from the gut of Apis mellifera L. Microbiol Discov. 2015;3(1):2. under solid state fermentation. Fermentation. 2016;2(4):10. ), production and characterisation and fruit juice application. Rom Biotechnol Lett. 2010;15:5167–76. -3. World J Microbiol Biotechnol. 2000;16(2):211–3. -UV37 using solid state fermentation. Springerplus. 2016;5:559. . Biocatal Agric Biotechnol. 2012;1(2):140–6. in solid state fermentation. Ann Microbiol. 2017;67(4):333–42. . Food Hydrocoll. 2015;43:819–24. in submerged and solid-state fermentations. Biomed Res Int. 20182018;2018:1514795. ) and its applications in fruit juice and oil extraction. Eur Food Res Technol. 2014;239(1):127–35. . Curr Agri Res Jour. 2018;6(1):37–44. 97 with applications in waste waters. Biocatal Agric Biotechnol. 2015;4(2):221–8. 0;15:56–63. 730. J Water Process Eng. 2021;43:102300. Article / Publication Details

First-Page Preview

Received: September 13, 2022
Accepted: November 16, 2022
Published online: November 23, 2022

Number of Print Pages: 15
Number of Figures: 9
Number of Tables: 3

ISSN: 2673-1665 (Print)
eISSN: 2673-1673 (Online)

For additional information: https://www.karger.com/MIP

Figures Tables