Effect of acute or subchronic stress on T cell response in peripheral blood: Regulatory role of vitamin D
Please use this identifier to cite or link to this item:
http://nopr.niscpr.res.in/handle/123456789/61133
metadata.dc.identifier.doi:
https://doi.org/10.56042/ijeb.v61i01.60922Title: Effect of acute or subchronic stress on T cell response in peripheral blood: Regulatory role of vitamin DAuthors:
Dogan, MuratSevgili, Ayse MeltemKozanoglu, IlknurPehlivanoglu, BilgeKeywords: Cold-immobilization stress;Interferon (IFN-γ);Interleukins;T helper cells;T regulatory cellsIssue Date: Jan-2023Publisher: NIScPR-CSIR,IndiaAbstract: The immune response, orchestrated by helper (Th1, Th2, and Th17) and regulatory (Treg) T cells, is modulated by stress
and Vitamin D (Vit-D). Although the immunomodulatory functions of both are known, their specific roles on Th cells have
not been fully clarified, yet. On this background, we aimed to investigate the effect of acute or subchronic stress on the
distribution of peripheral T lymphocytes, as well as the immunomodulatory role of Vit-D. Young adult male, Swiss-albino
mice (30–40g) were allocated to the control, acute stress (AS), subchronic stress (ChS), control+Vit-D, AS+Vit-D, and
ChS+Vit-D groups (n=11/group). The combined cold (2-h at 4°C)-immobilization (2-h in a restrainer) stress protocol was
employed as one day in AS groups and five consecutive days in ChS groups. Vit-D (2μg/kg ip) was applied every other day,
until the end of the protocol. Serum cortisol, Vit-D and cytokine levels (IL-4, IFN-γ, and IL-17A) were measured, and
lymphocytes from blood samples were subtyped by flow-cytometry. Stress exposure caused differential Th and Treg
responses, acute stress shifting the response to Th1, and subchronic stress shifting the response to Th2. Th17 and Treg cells
were lower in subchronic stress exposed mice. These changes became comparable to control values in Vit-D treated groups.
The T cell response, crucial for immune system function, differs on the basis of stress exposure as such the Vit-D treatment.
The tolerogenic profile created by Vit-D should be considered for management of stress-related diseases. Our results may
help to provide a better understanding of disease pathogenesis.Page(s): 14-24ISSN: 0975-1009 (Online); 0019-5189 (Print)Appears in Collections:
IJEB Vol.61(01) [Jan 2023]Items in NOPR are protected by copyright, with all rights reserved, unless otherwise indicated.
留言 (0)