Fusobacteria behaving badly: Masquerading strains of strictly anaerobic Escherichia coli misidentified due to the deletion of the hemB gene

Elsevier

Available online 27 December 2022, 102682

AnaerobeAuthor links open overlay panelAbstract

Three strictly anaerobic strains of Escherichia coli were misidentified as Fusobacterium mortiferum, due to a deletion of the hemB gene which is involved in anaerobic respiration. An unusual antimicrobial susceptibility pattern sparked the further diagnostic strategies that eventually identified these strains as true anaerobic E. coli This phenomenon is more common than appreciated and can have an impact on clinical practice including persistent and relapsing infections.

Section snippetsResults

The three variant Fusobacterium strains (anaerobic E. coli) were re-identified using 16S rDNA sequencing and found to match Escherichia coli with a 99.9% confidence level. The anaerobic strains were compared with their aerobic counterparts isolated from the same specimen by whole genome sequencing. After mapping to the K12 reference strain (ATCC 47076), single nucleotide polymorphism (SNP) calling identified 124,210 sites which defined the maximum likelihood phylogeny (Fig. 1). The SNPs

Declaration of competing interest

All authors had no conflict of interest.

There was no funding for this study.

Acknowledgements

All authors had no conflict of interests. There was no funding for this study.

References (12)J.S. Brazier et al.Artefactual resistance to metronidazole in anaerobes of the Bacteroides fragilis group

J. Antimicrob. Chemother.

(1992 Oct)

L.A. Lewis et al.Characterization of gentamicin-resistant respiratory-deficient (res---) variant strains of Staphylococcus aureus

Microbiol. Immunol.

(1990)

L.A. Lewis et al.Genetic and molecular analysis of spontaneous respiratory deficient (res-)mutants of Escherichia coli K-12

Microbiol. Immunol.

(1991)

D. Tappe et al.First case of febrile bacteremia due to a wild-type and small-colony variant of Escheria coli

Eur. J. Clin. Microbiol. Infect. Dis.

(2006)

Y.J. Park et al.Urinary tract infection caused by a small colony variant form of capnophilic Escherichia coli leading to misidentification and non-reactions in antimicrobial susceptibility tests

Antimicrob. Resist. Infect. Control

(2018 Nov 20)

P.N.A. Harris et al.Effect of Piperacillin-Tazobactam vs Meropenem on 30-day mortality for patients with E coli or Klebsiella pneumoniae bloodstream infection and ceftriaxone resistance: a randomized clinical trial

JAMA

(2018)

There are more references available in the full text version of this article.

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