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Figure 1. The establishment of the PAMs model with PRRSV-ADE infection. (A) viral loads of the cell culture supernatants in different infection groups were monitored by RT-qPCR. (B) Confirmation of the PRRSV proliferation in PAMs of the mock group, the PRRSV-NI group and the PRRSV-ICs group by IFA. The N protein (red) and the nucleus (blue) of these PAMs were counterstained. Scale bar = 50 μm. ** p < 0.01.
Figure 1. The establishment of the PAMs model with PRRSV-ADE infection. (A) viral loads of the cell culture supernatants in different infection groups were monitored by RT-qPCR. (B) Confirmation of the PRRSV proliferation in PAMs of the mock group, the PRRSV-NI group and the PRRSV-ICs group by IFA. The N protein (red) and the nucleus (blue) of these PAMs were counterstained. Scale bar = 50 μm. ** p < 0.01.
Figure 2. The volcano plot of DEPs in PAMs of the PRRSV-NI group and the PRRSV-ICs group. Upregulated proteins are shown in red, downregulated proteins are shown in blue and unchanged proteins are shown in gray.
Figure 2. The volcano plot of DEPs in PAMs of the PRRSV-NI group and the PRRSV-ICs group. Upregulated proteins are shown in red, downregulated proteins are shown in blue and unchanged proteins are shown in gray.
Figure 3. Subcellular annotation of the DEPs in PAMs infected with PRRSV-ADE infection. Upregulated proteins are shown in red, and downregulated proteins are shown in blue.
Figure 3. Subcellular annotation of the DEPs in PAMs infected with PRRSV-ADE infection. Upregulated proteins are shown in red, and downregulated proteins are shown in blue.
Figure 4. GO enrichment analysis of the DEPs in PAMs with PRRSV-ADE infection for biological process (A), molecular function (B) and cellular component (C). The abscissa in the figure is the rich factor which is the ratio of the number of different proteins in the corresponding pathway to the total number of proteins identified by the pathway. The color of the dot represents the p value of the hypergeometric test. The color ranges from green to red. The redder the color is, the smaller the value is. The size of the point represents the number of differential proteins in the corresponding pathway.
Figure 4. GO enrichment analysis of the DEPs in PAMs with PRRSV-ADE infection for biological process (A), molecular function (B) and cellular component (C). The abscissa in the figure is the rich factor which is the ratio of the number of different proteins in the corresponding pathway to the total number of proteins identified by the pathway. The color of the dot represents the p value of the hypergeometric test. The color ranges from green to red. The redder the color is, the smaller the value is. The size of the point represents the number of differential proteins in the corresponding pathway.
Figure 5. KEGG enrichment analysis of the DEPs in PAMs with PRRSV-ADE infection. Rich factor refers to the ratio of DEPs annotated in this pathway to all identified protein numbers annotated in this pathway. A larger rich factor with a lesser p value indicates a greater intensiveness. The top 26 enriched pathway terms were revealed.
Figure 5. KEGG enrichment analysis of the DEPs in PAMs with PRRSV-ADE infection. Rich factor refers to the ratio of DEPs annotated in this pathway to all identified protein numbers annotated in this pathway. A larger rich factor with a lesser p value indicates a greater intensiveness. The top 26 enriched pathway terms were revealed.
Figure 6. The PPI network of 143 DEPs. The connections between proteins indicate that they interact with each other and the confidence interval is greater than 0.9. DEPs were mainly concentrated in three PPI network clusters including ribosome, proteasome and mitochondria, which were circled with red, yellow and blue, respectively.
Figure 6. The PPI network of 143 DEPs. The connections between proteins indicate that they interact with each other and the confidence interval is greater than 0.9. DEPs were mainly concentrated in three PPI network clusters including ribosome, proteasome and mitochondria, which were circled with red, yellow and blue, respectively.
Figure 7. The validation of the DEPs in PAMs with PRRSV-ADE infection. (A) RT-qPCR analysis of six selected DEPs (COX-5B, MMP-9, ISG56, OAS1, Mx1 and RSAD2) in PAMs with PRRSV-ADE infection. (B) Western blot analysis of four DEPs (TBK-1, STAT-1, STAT-2 and Mx1) in PAMs with PRRSV-ADE infection. *** p < 0.001.
Figure 7. The validation of the DEPs in PAMs with PRRSV-ADE infection. (A) RT-qPCR analysis of six selected DEPs (COX-5B, MMP-9, ISG56, OAS1, Mx1 and RSAD2) in PAMs with PRRSV-ADE infection. (B) Western blot analysis of four DEPs (TBK-1, STAT-1, STAT-2 and Mx1) in PAMs with PRRSV-ADE infection. *** p < 0.001.
Table 1. Forty DEPs associated with antiviral innate immune signals identified in this study.
Table 1. Forty DEPs associated with antiviral innate immune signals identified in this study.
AccessionProtein NameGene NameFold Change (PRRSV-ICs/PRRSV-NI)p ValueA0A0B8RS69Tripartite motif-containing protein 34 (TRIM34)TRIM340.7005587020.008236 A0A0B8RT27Toll-like receptor 3 (TLR3)TLR31.4800821420.000012 A0A0B8S066Interferon regulatory factor 9 (IRF9)IRF90.6458525020.001426 A0A286ZN85Signal transducer and activator of transcription 1 (STAT-1)STAT-10.6790899350.000960 A0A287A5V9DEAD-box protein 6 (DDX6)DDX60.5777784350.002041 A0A287AF06Interleukin-1 receptor-associated kinase-like 2 (IRAK2)IRAK20.7073946090.016606 A0A287ANQ6Tripartite motif-containing protein 52 (TRIM52)TRIM521.5793222180.001208 A0A287AVQ1DEAD-box protein 3X (DDX3X)DDX3X0.6348287740.000292 A0A385XIH52’-5’ oligoadenylate synthase 1 (OAS1)OAS10.6437308960.002701 A0A4X1SHM4Tripartite motif-containing protein 25
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