Figure 1. Algorithm from Venezuelan INHRR for the molecular detection of MPXV cases. Samples from patients with history of international travel were directly tested for the presence of MPXV DNA.

Figure 2. Phylogenetic analysis of the first case pf MPXV in Venezuela. The different lineages described for clade IIb are shown.

Table 1. Primers used for amplification of MPXV.

Table 1. Primers used for amplification of MPXV.

PCRPrimerSequenceReferenceqPCR (generic)G2R_GF 5′-ggaaaatgtaaagacaacgaatacag-3´[6] G2R_GR 5′-gctatcacataatctggaagcgta-3´ G2R_G probe5′FAM-aagccgtaatctatgttgtctatcgtgtcc-3′BHQ1 qPCR (Clade II)G2R_WAF5′-cacaccgtctcttccacaga-3´[6] G2R_WAR5′-gatacaggttaatttccacatcg-3´ G2R_WA probe5′FAM-aacccgtcgtaaccagcaatacattt-3′BHQ1 PCR (ORF F4L)F4LF5´cgttggaaaacgtgagtccgg-3´[7]Pox genusF4LR5´-attggcgttttttgcagccag-3´ PCR (E5R)E5RF5´-atgttgatattaataatcgtattgtggtt-3´[7] E5RR5´-aaagtcaatacactcttaaagattctcaa-3´ PCR (HA)HAOUTF5´-ccattggaaaaaacacagtac-3´[8] HAOUTR5´-ccaaatatattcccatagtc-3´