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Chemicals
Collection and extraction of plant material
Determination of total phenols
Determination of total flavonoids
Determination of antioxidant activity using the DPPH assay
Identification of phenols and flavonoids using HPLC/MS-MS
Experimental animals
Induction of nephrotoxicity
Experimental animals (in vivo study)
Urine, blood and kidney samples collection
Biochemical data measurements
Histological studies
Statistical analysis
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