The non-lytic release of ATP occurs in lymphoid tissues at a steady state.

Purinergic signaling is induced by regulated ATP release from naïve T cells in response to chemokines.

Extracellular ATP has a homeostatic role in immune cell migration in lymph nodes.

Antigen stimulation induces adenosine triphosphate (ATP) release from naïve lymphocytes in lymphoid tissues. However, previous studies indicated that the non-lytic release of ATP also occurs in most tissues and cell types under physiological conditions. Here, we show that extracellular ATP (eATP) is indeed constitutively produced by naïve T cells in response to lymphoid chemokines in uninflamed lymph nodes and is involved in the regulation of immune cell migration. In this review, we briefly summarize the homeostatic role of extracellular ATP in immune cell migration in vivo.

ATP

Purinergic receptor

Lymphocyte

Chemokine

Cell migration

adenosine triphosphate

high endothelial venule

sphingosine-1-phosphate

matrix-assisted laser desorption/ionization imaging mass spectrometry

capillary electrophoresis-electrospray ionization mass spectrometry

© 2022 The Author(s). Published by Elsevier Ltd.