Anti-TGF-{beta}/PD-L1 bispecific antibody promotes T cell infiltration and exhibits enhanced antitumor activity in triple-negative breast cancer

Background

Breast cancer is the leading threat to women’s health, causing nearly 68,500 deaths globally in 2020.1 2 According to the status of hormone receptors and human epidermal growth factor 2 (HER2), breast cancers are classified into four subtypes: luminal A/B, HER2-enriched, normal-like, triple-negative breast cancer (TNBC). TNBC is the most aggressive breast cancer subtype, lacking specific targets for effective therapeutic agents. Therefore, there is a critical need for innovative treatment strategies for TNBC. Immunotherapy, especially anti-programmed cell death protein 1/programmed death-ligand 1 (anti-PD-1/PD-L1), revolutionizes multiple cancers’ treatment paradigm.3–7 Currently, pembrolizumab combined with chemotherapy has been approved for TNBC.8 9 Nevertheless, the low response rate of anti-PD-1/PD-L1 therapy has not been well resolved.10 The therapeutic effect of anti-PD-1/PD-L1 is hampered by multiple immunosuppressive factors, including transforming growth factor-beta (TGF-β).11

As a multifunctional cytokine, the function of TGF-β is context-dependent. For normal tissue and early-stage breast cancer, TGF-β plays as a tumor suppressor by inducing cell-cycle arrest and apoptosis.12 However, for late-stage breast cancer, TGF-β promotes tumor progression by enhancing metastasis, radiotherapy or chemotherapy resistance, and the tumor microenvironment (TME) reprogramming.13–16 The immunosuppressive effects of TGF-β on the TME are multifaceted: impairing the activities of tumor-infiltrating lymphocytes (TILs), altering the polarization of macrophages, inducing regulatory T (Treg) cell differentiation, and limiting dendritic cell (DC) functions.17 18 Moreover, TGF-β undermines TIL penetration by increasing peritumoral collagen production of cancer-associated fibroblast (CAF).19

Accumulating data demonstrate high TGF-β is correlated with resistance to anti-PD-1/PD-L1 therapy,19 while neutralizing TGF-β improves the efficacy of anti-PD-1/PD-L1.19–21 In the previous study, we designed and constructed the bispecific antibody (BsAb) blocking TGF-β and murine PD-L1 (named YM101) based on Check-BODY platform. YM101 showed potent antitumor activity without observable toxicity in murine tumor models.22–24 Given the success of YM101 in the pilot study, we further developed the BsAb targeting TGF-β and human PD-L1 (termed BiTP) using Check-BODY platform. BiTP was constructed by modifying the structure of YM101, aiming to provide potential anti-TGF-β/PD-L1 BsAb for future clinical trials. Here, we measured the biochemistry characteristics and bioactivities of BiTP. In addition, we investigated the antitumor activity of BiTP in TNBC models and its underlying mechanisms.

Materials and methodsCell lines and antibodies

Human peripheral blood mononuclear cells (hPBMCs), H358, NK92MI engineered to express CD16A (NK92MI-CD16A), A549, MCF7, BT474, 4T1-hPDL1, epithelial-mesenchymal transition (EMT)-6-hPDL1, and Jurkat-1-PD-1-NFAT-Luc were cultured in RPMI-1640 medium with 10% fetal bovine serum (FBS) (Excell). TF-1 was cultured in RPMI-1640 medium with 10% FBS and 2 ng/mL recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF) (7954 GM, R&D). CHO-K1 cell engineered to express PD-L1 and anti-CD3 scFv (CHO-K1-PD-L1-CD3L) and HFL1 (human fetal lung fibroblast) were cultured with Ham’s F-12K medium with 10% FBS. Primary human fibroblast was maintained in DEME medium with 10% FBS. Therapeutic antibodies used in this work were purchased from Wuhan YZY Biopharma.

Molecular weight and purity test

The molecular weight and purity of BiTP were estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Coomassie Brilliant Blue staining, as previously described.25 The exact molecular weight was measured by liquid chromatograph-mass spectrometer, and dithiothreitol was added to detect the molecular weight of single-chain.26 According to the standard protocol, the exact purity was tested by capillary electrophoresis-SDS (CE-SDS).27 Iodoacetamide was used for non-reduced CE-SDS, while β-mercaptoethanol was used for reduced CE-SDS. Prepared mixture for CE-SDS was diluted in SDS-MW buffer, with a total volume of 101 µL. After incubation at 70℃ for 10 min, we performed CE-SDS separations in Beckman PA 800 plus platform.

Biacore surface plasmon resonance assay

The affinity of BiTP was measured by surface plasmon resonance (SPR) based on a Biacore T200 Biosensor instrument (GE Healthcare) with BiTP captured on Protein A chip. Different concentrations of TGF-β1 or PD-L1 (Wuhan YZY Biopharma) were injected to test the kinetical binding interaction. The results of SPR were further processed with Biacore T200 Biaevaluation V.2.0 software. Finally, we used a 1:1 binding model to calculate the association rate constant (Kon), dissociation rate constant (Koff), and equilibrium dissociation constant (Kd) in the assays.

ELISAs using plate-bound TGF-β

To detect the binding affinity of the anti-TGF-β moiety, we precoated plates with TGF-β (200 ng per well) at 37℃ for 2 hours. Next, the plates were washed using PBST and blocked using 5% bovine serum albumin (BOVOGEN) for 2 hours. Then, the plates were washed and probed with serially diluted BiTP for 1 hour. After washing with PBST, the secondary antibody (anti-hIgG-HRP, 1:5000, BOSTER) was added. In accordance with the standard ELISA protocol, the absorbance was detected at 450 nm.

To detect the simultaneous binding affinity of BiTP, we performed double-antigen sandwich ELISA. Plates were precoated with 100 ng TGF-β1 at 37℃ for 2 hours. After washing and blocking, plates were probed with serially diluted BiTP at 37°C for 1 hour. Following incubation and washing, PD-L1-HRP (5000 ng/mL, 100 µL per well, Wuhan YZY Biopharma) was added. The simultaneous binding of BiTP was quantified using absorbance at 450 nm.

ELISAs determining the binding of BiTP to human Fc gamma receptors (hFcγRs)

Plates were precoated with hFcγRI, hFcγRIIA, hFcγRIIB, hFcγRIIIA, and hFcγRIIIB (Acro) (100 ng per well) at 37°C for 2 hours. After washing and blocking using 5% BSA, the assay plates were probed with serially diluted BiTP for 1 hour. Anti-hFab-HRP (A0293, Sigma) was used as the secondary antibody. The absorbance value was detected at 450 nm.

In vitro antibody-dependent cellular cytotoxicity detection

H358 cells were stained with 2.5 µM carboxyfluorescein diacetate succinimidyl ester buffer (containing 1% FBS) at 37°C for 15 min. Then, H358 cells were washed by medium and seeded into 96 well plates (2×104 per well). The next day, NK92MI-CD16A (4×104 per well) and antibodies were added to plates. Cells were cultured at 37°C for 6 hours. After treatment with propidium iodide (1 µg/mL, P4170-25 mg, Sigma), the ratio of dead cells was measured by flow cytometry.

Cell-binding assay using flow cytometry

H358 cells were incubated with serially diluted BiTP at room temperature for 1 hour. After thoroughly washing, cells were incubated with APC-conjugated anti-hIgG Fc antibody (409306, BioLegend) for 30 min. The cell-binding activity was measured by mean fluorescence intensity.

BiTP competitively inhibited the binding of PD-1 and TGFBR2 to corresponding ligands

Plates were precoated with TGF-β1 (200 ng per well) at 37°C for 2 hours. After washing and blocking, the plates were added with TGFBR2-HIS (Wuhan YZY Biopharma) (5 ng/mL for TGF-β1, 10 µg/mL for TGF-β2, 0.14 µg/mL for TGF-β3) and serially diluted BiTP. Then, the plates were incubated with the secondary antibody (1:5000) (HRP-66005, Proteintech) for 1 hour. The absorbance value was detected at 450 nm. For the competitive inhibition of binding between PD-1 and PD-L1, plates were precoated with 200 ng PD-1-Fc (10377-H02H, Sino Biological). PD-L1-HIS (1 µg/mL, 10084-H08H, Sino Biological) and serially diluted BiTP were used in this assay.

Pharmacokinetics

CD-1 mice were used for the pharmacokinetic study. Mice received 3 mg/kg, 9 mg/kg, and 27 mg/kg BiTP treatment by intravenous injection. Then, 5 min, 2 hours, 8 hours, 24 hours, 3 days, 7 days, 14 days, 21 days, and 28 days later, 0.6 mL peripheral blood was collected. The concentration of BiTP in plasma was measured by ELISA assays. Goat anti-human IgG HRP Conjugated Min X Monkey (A80-319P, BETHYL) was used in this assay.

The influence of BiTP on the concentration of TGF-β in plasma

B-cell-depleted (μMT) mice were used to investigate the effect of BiTP treatment on TGF-β plasma concentration. Mice received 9 mg/kg BiTP and 6.6 mg/kg anti-PD-L1 by intravenous injection. Before treatment, as well as 2 hours, 6 hours, 24 hours, 48 hours, 72 hours, 120 hours, and 168 hours after treatment, peripheral blood was collected to measure the concentration of TGF-β. The level of TGF-β was detected using U-PLEX TGF-β Mouse SECTOR (K15242K-1, MSD) and MESO QuickPlex SQ 120 (MSD) according to the manufacturer’s recommendations.

CCK-8 assay

TF-1 proliferation is sensitive to TGF-β.28 We used CCK-8 reagent (Dojindo) to determine the capability of BiTP against the TGF-β-inhibited proliferation of TF-1 cells. 3×103 viable TF-1 cells were seeded in 96-well plates. Then, TGF-β (TGF-β1: 1 ng/mL; TGF-β2: 20 ng/mL; TGF-β3: 1 ng/mL) and antibodies were added. After culture for 6 days, CCK-8 reagent was added to assay plates. Following incubation for 3 hours, the absorbance values were measured by microplate reader (Molecular Devices).

Immunofluorescence

Before treatment, human primary fibroblast and HFL1 were cultured with the DMEM or Ham’s F-12K containing 1% FBS for 24 hours. Then, the medium was replaced with DMEM or Ham’s F-12K containing 1% FBS, 10 ng/mL TGF-β1, and 105 pM BiTP. After incubation for 72 hours, the cells were fixed with 4% paraformaldehyde for 30 min, permeabilized with 1% Triton-X 100 for 20 min, and incubated with 5% BSA for 1 hour. Then, the cells were incubated with primary antibodies (anti-α-SMA, 19245, CST, 1:300; anti-Collagen I, ab34710, Abcam, 1:300) at 4℃ overnight. The fluorescent secondary antibody (ab150077, Abcam, 1:300) was incubated at room temperature for 1 hour. The cell nuclei were counterstained with DAPI (4083, CST, 1 µg/mL).

Western blotting

Western blotting was performed as previously described.26 The following primary and secondary antibodies were used (all antibodies from CST): anti-E-cadherin (1:1000, 3195), anti-N-cadherin (1:1000, 13116), anti-vimentin (1:1000, 5741), anti-β-Actin (1:1000, 8457), and anti-rabbit-IgG-HRP antibody (1:3000, 7074).

Treg induction assay

Plates were coated with 10 µg/mL anti-human CD3 (Clone OKT3, BioLegend) at 37°C for 2 hours. The hPBMCs were maintained in RPMI-1640 with 10% FBS, 100 U/mL interleukin-2 (IL-2, Beijing Fourrings), 5 µg/mL anti-CD28 (Clone 28.2, BioLegend), 20 ng/mL TGF-β1, and BiTP for 6 days.

Cytokine detection assay

To measure the bioactivity of the anti-PD-L1 moiety of BiTP, we conducted a superantigen stimulation assay. Viable hPBMCs (5×104 /mL) were mixed with serially diluted antibodies. Staphylococcal enterotoxin A (500 ng/mL, Toxin Technology) was added to the hPBMC-antibody mixtures. Four days later, IL-2 concentration in the supernatants was detected by Multi-Analyte Flow Assay Kit (Human CD8/NK Panel, BioLegend).

Smad luciferase reporter assay

A549 cells were plated in 96-well plates (2×104 cells per well) on the day before transfection. Then, transient transfection with Smad luciferase reporter plasmid was performed by QIAGEN Effectene Transfection Reagent. Subsequently, cells were incubated with 20 ng/mL TGF-β1 and serially diluted antibodies for 24 hours. Luminescence was measured by Promega Bright-Glo Luciferase Assay System according to the manufacturer’s recommendations.

NFAT luciferase reporter assay in CHO-K1 cells

CHO-K1-PD-L1-CD3L cells (4×104 per well) were seeded in 96 well plates and cultured for 16 hours. Then, Jurkat-1-PD-1-NFAT-Luc cells (5×104 cells per well) and serially diluted antibodies were added to plates. After incubation for 6 hours, luminescence was measured by Promega Bright-Glo Luciferase Assay System.

Murine tumor models

Humanized TNBC models (EMT-6-hPDL1 and 4T1-hPDL1) were used to investigate the antitumor efficacy of BiTP. The endogenous mouse Cd274 of TNBC cells was replaced by human CD274. Due to the interaction between hPD-L1 and mouse PD-1 (mPD-1), this hPD-L1 expressing tumor model could be adopted to evaluate the efficacy of anti-hPD-L1 antibodies.29 Totally, we established two orthotopic breast cancer models (EMT-6-hPDL1 and 4T1-hPDL1) by inoculating 1×105 tumor cells into the right mammary fat pad of BALB/c mice. When tumor volume was nearly 100 mm3, tumor-bearing mice were randomized into four groups (n=8) and treatment started: Isotype (i.p., 6.6 mg/kg), anti-TGF-β (i.p., 6.6 mg/kg), anti-PD-L1 (i.p., 6.6 mg/kg), and BiTP (i.p., 9 mg/kg). All mice received six doses of treatment in the following 2 weeks. Tumor volume (length×width2×0.5) and mouse weight were recorded on alternate days. Mice were euthanized when the study ended or tumor volume exceeded 2500 mm3.

Flow cytometry for the TME

Tumors were collected, minced, and digested with collagenase B (0.5 mg/mL, Roche) and hyaluronidase (0.5 mg/mL, Absin) at 37°C for 1 hour. Suspensions were filtered through 40 µm cell strainers. Then, cells were suspended and stained with Fixable Viability Stain 700 (BD Biosciences). Cells were stained according to the standard protocol for flow cytometry. The following antibodies and buffers were used (all reagents from BD Biosciences unless otherwise indicated): anti-CD45 (560510), anti-CD3e (562600), anti-CD8α (563068), anti-CD25 (553075), anti-CD69 (566500), anti-Ki67 (556027), anti-TNF-α (563943), anti-IFN-γ (560660), anti-Perforin (ThermoFisher, 11-9392-82), anti-Granzyme-B (BioLegend, 372204), anti-CD11c (566504), anti-I-A/I-E (BioLegend, 107608), anti-CD80 (560016), anti-CD86 (561962), Brilliant Stain Buffer (563794), and FOXP3/Transcription Factor Staining Buffer Set (Invitrogen, 00-5523-00). Cells per 100 mg tumor were counted by Beckman Vi-Cell Auto. Flow cytometry was performed with Beckman CytoFLEX LX.

Immunohistochemistry and multiplex immunohistochemistry

Tumor tissues were fixed with 4% paraformaldehyde at room temperature for 2 days, dehydrated, and embedded in paraffin wax. Primary antibodies used in this study included antibodies recognizing CD8 (Abcam, ab217344), Vimentin (CST, 5741), E-cadherin (CST, 3195), α-SMA (CST, 19245), Cleaved-Caspase-3 (CST, 9661), Ki67 (Abcam, ab16667), and PCNA (CST, 13110). Direct Red 80 (Sigma, 365548) was used for picrosirius red staining. The fluorescent multiplex immunohistochemistry (IHC) staining with Tyramide Signal Amplification was conducted as previously described.30 IHC staining was performed following a two-step protocol.31 Two pathologists independently defined the regions of interest. IHC staining was scored using ImageJ software. The expression levels were quantified with integral optical density. T cell infiltration depth was measured by radius-scaled distance from CD8+ pixels to margin.19

Bulk RNA-seq assay

The bulk RNA-seq assay was performed by Seqhealth (Wuhan, China) (online supplemental table S1). Total RNA was extracted from the 4T1-hPDL1 tumor tissues by TRIzol reagent (Invitrogen, 15596026).31 After RNA extraction, DNA digestion was performed using DNaseI. RNA quality was assessed by A260/A280 with Nanodrop One Cspectrophotometer (Thermo Fisher). RNA integrity was measured with 1.5% agarose gel electrophoresis. Then, qualified RNA was quantified by Qubit3.0 with Qubit RNA Broad Range Assay kit (Life Technologies, Q10210).

Subsequently, 2 µg total RNA was used for stranded RNA sequencing library preparation with Ribo-off rRNA Depletion Kit (Illumina, MRZG12324) and KC-Digital Stranded mRNA Library Prep Kit (Wuhan Seqhealth, DR08502) following the manufacturer’s instruction. This kit labels the preamplified cDNA molecules with unique molecular identifier of 8 random bases, in order to eliminate duplication bias in PCR and sequencing steps. The library products corresponding to 200–500 bps were enriched, quantified, and sequenced on NovaSeq 6000 sequencer (Illumina) with PE150 model.

Deduplicated Reads were mapped to the reference genome of Mus_musculus.GRCm38 (https://ftp.ensembl.org/pub/release-87/fasta/mus_musculus/dna/) with the annotation from gff3 file (https://ftp.ensembl.org/pub/release-87/gff3/mus_musculus/) by STRA analysis tool (2.5.3a) using default parameters. Then, reads mapping to exons were counted using featureCounts tool (Subread-1.5.1; Bioconductor) and scaled with RPKM (Reads Per Kilobase per Million mapped reads) method. After assessing RNA-seq performance and quality (online supplemental table S2), a standard analysis pipeline was adopted to detect the biological signals of samples. Differentially expressed gene (DEG) was identified when the gene presented >2 fold expression difference and adjusted p<0.05. The DEG analysis was conducted by DESeq2 package (1.34.0).32 The results of DEG analysis were visualized by pheatmap package (1.0.12).33 The principal component analysis was performed using prcomp function of stats package. The functional enrichment was performed using the online tool The Database for Annotation, Visualization and Integrated Discovery. A pathway with adjusted p value (false discovery rate (FDR))<0.05 is regarded as statistically significant. According to public gene lists, immune signatures were constructed and scored.34 Briefly, signature scores were performed by calculating the mean value of scaled gene expression levels.

Statistical analyses

Statistical analyses were conducted by R software and GraphPad Prism V.8 software. Student’s t-test with or without Welch’s correction and Mann-Whitney test were adopted to compare two groups. Survival curves were compared by log-rank test. Data were presented as mean ±SE of mean (SEM) or SD. All tests were two sided, and p<0.05 was regarded as significant.

ResultsThe structure of BiTP

As an IgG1/IgG2 hybrid antibody, the Fc of BiTP contains IgG2-derived CH2 domain and IgG1-derived CH3 domain, with D270A modification to reduce the binding affinity to FcγR (online supplemental figure S1A–E). We did not observe significant antibody-dependent cell-mediated cytotoxicity for BiTP (online supplemental figure 1). The VLa, CL, VHa, and CH1 are derived from the corresponding domains of anti-human PD-L1. The VHb and VLb domains are derived from the corresponding domains of anti-TGF-β (figure 1A). Reduced SDS-PAGE showed BiTP consists of short chain and long chain (figure 1B). CE-SDS demonstrated the purity was more than 97% (figure 1c). The molecular weight of BiTP is about 201.5 KDa (short chain: 37.3 KDa; long chain: 63.4 KDa) (online supplemental figure S2A–C).

Figure 1Figure 1Figure 1

The structure and binding affinity of BiTP. (A) The structure of BiTP. BiTP is an IgG1/IgG2 hybrid antibody, containing IgG2-derived CH2 domain and IgG1-derived CH3 domain. The VLa, CL, VHa, and CH1 are derived from the corresponding domains of anti-PD-L1. The VHb and VLb domains are derived from the corresponding domains of anti-TGF-β. (B) Non-reduced and reduced SDS-PAGE. (C) Non-reduced and reduced CE-SDS. (D–F) The binding affinity to TGF-β1. BiTP was captured by plate-coated TGF-β. The affinity was determined by ELISA. (G) The binding affinity to PD-L1. Antibodies were incubated with H358 cells. The binding affinity was measured by mean fluorescence intensity in flow cytometry assay. (H) The simultaneous binding to PD-L1 and TGF-β1. BiTP was captured by precoated TGF-β1. Then, PD-L1-HRP was added, and the simultaneous binding was detected by ELISA. CE-SDS, capillary electrophoresis-sodium dodecyl sulfate; MFI, mean fluorescence intensity; TGF-β, transforming growth factor-beta.

Figure 2Figure 2Figure 2

Competitive binding experiments and pharmacokinetic study. (A–C) BiTP competitively inhibited the binding of TGFBR2 to TGF-β. TGFBR2-HIS and BiTP were incubated with precoated TGF-β. The competitive inhibition capability was evaluated with anti-HIS ELISA. (D) BiTP competitively inhibited the binding of PD-1 to PD-L1. PD-L1-HIS and BiTP were incubated with precoated PD-L1. The competitive inhibition capability was evaluated with anti-HIS ELISA. (E) CD-1 mice received 3 mg/kg, 9 mg/kg, 27 mg/kg BiTP treatment by intravenous injection. Then, 5 min, 2 hours, 8 hours, 24 hours, 3 days, 7 days, 14 days, 21 days, and 28 days later, 0.6 mL peripheral blood was collected to measure the concentration of BiTP in plasma. (F–H) The influence of BiTP on the concentration of TGF-β in plasma. B-cell-depleted (μMT) mice received 9 mg/kg BiTP and 6.6 mg/kg anti-PD-L1 treatment. Before treatment, as well as 2 hours, 6 hours, 24 hours, 24 hours, 72 hours, 120 hours, and 168 hours after treatment, peripheral blood was collected to measure the concentration of TGF-β. TGF-β, transforming growth factor-beta.

The binding affinity of BiTP

Compared with anti-TGF-β, BiTP bound to plate-bound TGF-β with similar or slightly weaker affinities (TGF-β1: Kd=101 pM for BiTP, 160 pM for anti-TGF-β; TGF-β2: Kd=3.59×103 pM for BiTP, 2.17×103 pM for anti-TGF-β; TGF-β3: Kd=2.76×105 pM for BiTP, 7.51×104 pM for anti-TGF-β) (figure 1D–F). In addition, BiTP bound to H358 cells (expressing a high level of PD-L1) with a similar affinity to anti-PD-L1 (Kd=133 pM for BiTP, 119 pM for anti-PD-L1) (figure 1G). Double-antigen sandwich ELISA showed that TGF-β1-captured BiTP simultaneously bound to PD-L1 (Kd=68 pM) (figure 1H).

In addition, we used SPR Biacore assays to measure the kinetic binding of BiTP to TGF-β (Kon=3.20×106 M−1S−1, Koff=4.17×10−3 S−1, Kd=1.30×10−9 M) and PD-L1 (Kon=2.39×105 M−1S−1, Koff=3.48×10−4 S−1, Kd=1.45×10−9 M) (online supplemental figure S3A,B). The results of competitive binding assays showed that a high level of BiTP could competitively antagonize the binding between TGF-β1 and TGFBR2 (IC50=2.13×104 pM). However, BiTP had a modest effect on the binding of TGF-β2 or TGF-β3 to TGFBR2. Generally, the competitive binding property of BiTP was weaker than anti-TGF-β (figure 2A–C). Moreover, a high concentration of BiTP almost completely inhibited the binding of PD-1 to PD-L1 (IC50=3.84×103 pM) (figure 2D).

Figure 3Figure 3Figure 3

The in vitro bioactivity of BiTP. (A–C) CCK-8 assays to detect the capability of BiTP against TGF-β-inhibited proliferation of TF-1 cells. (D) Luciferase reporter experiments to evaluate Smad-mediated transcriptional activity. Smad-Luc-transfected A549 cells were treated with 20 ng/mL TGF-β1 and antibodies for 24 hours. Then, luminescence was detected. (E) The diagram showing NFAT luciferase reporter system. In the system, Jurkat-1-PD-1-NFAT-Luc and CHO-K1-PD-L1-CD3L were used. The activity of NFAT-Luc could be hampered by PD-1-PD-L1 axis. (F) NFAT luciferase reporter experiments to detect PD-1 signaling. Jurkat-1-PD-1-NFAT-Luc and BiTP were incubated with CHO-K1-PD-L1-CD3L for 6 hours. Then, luminescence was detected. (G) Superantigen stimulation assay assessing the activity of the anti-PD-L1 moiety of BiTP. PBMCs were mixed with antibodies and staphylococcal enterotoxin A (SEA). Four days later, IL-2 concentration in the supernatants was detected. PBMCs, peripheral blood mononuclear cell; TGF-β, transforming growth factor-beta.

Pharmacokinetic study and TGF-β concentration in plasma

BiTP exhibited nonlinear pharmacokinetics in CD-1 mice. The half-life time (t1/2) of BiTP was 34 hours at the dose of 9 mg/kg (figure 2E). In μMT mice, after a single dose of BiTP, the TGF-β1 and TGF-β2 in plasma were almost completely depleted (from 2 to 120 hours) (figure 2F–G). In addition, after BiTP treatment, the concentration of TGF-β3 was maintained at a low level throughout all time points (figure 2H).

The bioactivity of BiTP’s anti-TGF-β moiety

The biological activity of TGF-β could be determined by TF-1 proliferation assay.35 TGF-β suppressed GM-CSF-dependent TF-1 proliferation in vitro. The TGF-β-caused impairment on TF-1 proliferation could be reversed by BiTP in a dose-dependent manner (EC50=148 pM for TGF-β1, 1.41×104 pM for TGF-β2, 939 pM for TGF-β3) (figure 3a–3c). In addition, BiTP effectively blocked canonical TGF-β-Smad signaling (EC50=92 pM) (figure 3d).

Then, we explored the antagonism of BiTP on TGF-β-mediated immune and nonimmune effects. Exogenous TGF-β1 promoted Treg differentiation in vitro. Different from isotype and anti-PD-L1, BiTP effectively inhibited TGF-β1-induced Treg differentiation (online supplemental figure S4A). In addition, in line with previous reports, TGF-β1 downregulated epithelial marker E-cadherin and increased mesenchymal markers N-cadherin and Vimentin. BiTP effectively reversed TGF-β1-induced EMT in breast cancer cells: increasing epithelial marker and decreasing mesenchymal markers (online supplemental figure S4B).

Figure 4Figure 4Figure 4

The antitumor activity of BiTP in murine TNBC models. (A) The representative images EMT-6-hPD-L1 implants in mice receiving antibody treatment. (B) The growth curves of EMT-6-hPD-L1 implants in mice receiving antibody treatment. (C) The weights of EMT-6-hPD-L1 implants in mice receiving antibody treatment. (D) The representative images 4T1-hPD-L1 implants in mice receiving antibody treatment. (E) The growth curves of 4T1-hPD-L1 implants in mice receiving antibody treatment. (F) The weights of 4T1-hPD-L1 implants in mice receiving antibody treatment. (G, H) Survival of tumor-bearing mice receiving antibody treatment. (I, J) Body weight changes of tumor-bearing mice following treatment. (K–M) Rechallenge assays. BALB/c mice were orthotopically inoculated with 105 tumor cells on day 0, and BiTP therapy was initiated on day 4. On day 21, BiTP-cured and treatment-naive mice were inoculated with 105 tumor cells for rechallenge assays. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 mean the significant difference compared with BiTP-treated samples. EMT, epithelial-mesenchymal transition; TNBC, triple-negative breast cancer.

The bioactivity of BiTP’s anti-PD-L1 moiety

The bioactivity of BiTP’s anti-PD-L1 moiety was assessed by a luciferase reporter system containing Jurkat-1-PD-1-NFAT-Luc and CHO-K1-PD-L1-CD3L cells (figure 3E). BiTP counteracted PD-1/PD-L1 pathway-mediated suppression on NFAT signaling (EC50=240 pM) (figure 3F). In the superantigen stimulation assay, BiTP enhanced the secretion of IL-2 (figure 3G). This enhancement of T cell activity was related to the dose of BiTP (EC50=16.81 pM). The high dose of BiTP exhibited a stronger capability to promote T cell activation.

The antitumor activity of BiTP in murine models

The antitumor effects of BiTP were evaluated in the EMT-6-hPDL1 and 4T1-hPDL1 models. One week after tumor cell inoculation, mice were grouped and treated with six doses of antibodies. In two TNBC models, BiTP exhibited superior antitumor activity to parent antibodies. Compared with other three groups, BiTP also significantly slowed tumor growth and extended the survival of tumor-bearing mice (figure 4A–H). In addition, we did not observe overt toxicity and weight loss in mice receiving antibody treatment, indicating the acceptable safety and tolerability of BiTP (figure 4I,J). Rechallenge assays showed all BiTP-cured mice rejected rechallenged tumors, demonstrating BiTP established a potent antitumor immune memory (figure 4K–M).

BiTP suppressed collagen production and promoted T cell infiltration

Our previous study showed that TGF-β blockade enhanced T cell infiltration by limiting CAF-derived collagen.22 Given that CAF-derived collagen undermines T cell penetration, we first explored the effects of BiTP on CAF activity in vitro. α-SMA is the marker of CAF.36 For primary fibroblast and HFL1, TGF-β1 increased α-SMA expression and collagen I generation (figure 5A). Meanwhile, BiTP treatment antagonized the upregulation of α-SMA and collagen I caused by TGF-β1.

Figure 5Figure 5Figure 5

The effects of BiTP on collagen production and CD8+ T cell infiltration in vivo. (A) The effect of BiTP on TGF-β-mediated fibroblast activation. Primary human fibroblast from gastric tissue and HFL1 cells were cultured with TGF-β1 and antibodies. Immunofluorescent staining was performed to measure the levels of α-SMA and collagen I. (B) Multiplex IHC staining to evaluate the level of α-SMA in 4T1-hPD-L1 tumors. (C) Picrosirius red staining to measure collagen production in 4T1-hPD-L1 tumors. (D) Multiplex IHC staining to evaluate the infiltration of CD8+ T cell. The presentative images of infiltrating CD8+ T cells. (E, F) The quantification of anti-α-SMA and picrosirius red staining. (G, H) The quantification of CD8+ pixel and infiltration depth. *p < 0.05, ***p < 0.001, and ****p < 0.0001 mean the significant difference compared with BiTP-treated samples. HFL, human fetal lung fibroblast; IHC, immunohistochemistry; TGF-β, transforming growth factor-beta.

Next, we evaluated CAF activity and CD8+ T cell infiltration in 4T1-hPD-L1 tumors. Anti-α-SMA staining showed BiTP and anti-TGF-β downregulated α-SMA expression, indicating restrained CAF activity (figure 5B). Picrosirius red staining showed that collagen production was significantly decreased after BiTP and anti-TGF-β treatment (figure 5C). Then, we performed anti-CD8 staining to investigate the immune infiltration status. For mice treated with hIgG or anti-PD-L1, most CD8+ pixels were located in tumor margin. In contrast, for mice treated with anti-TGF-β or BiTP, more CD8+ pixels were in tumor center (figure 5D). Quantitative analysis showed that BiTP expanded T cell population and increased its infiltration depth. Collectively, BiTP suppressed CAF activity, reduced collagen deposition, and enhanced T cell infiltration (figure 5E–H).

Flow cytometry revealing alterations in the TME after BiTP treatment

Subsequently, we collected 4T1-hPDL1 tissues to detect changes in immune populations by flow cytometry (figure 6A–C). Compared with the other three groups, BiTP significantly increased TIL, CD8+ T cell, activated (CD69+/CD25+) CD8+ T cell, proliferating (Ki67+) CD8+ T cell, cytotoxic (Granzyme-B+/Perforin+/TNF-α+) T cell, NK cell, proliferating (Ki67+) NK cell, and cytotoxic (granzyme-B+/IFN-γ+/Perforin+/TNF-α+) NK cell (figure 6D–Q). In addition to TIL, BiTP also expanded total DC and mature (CD80+/CD86+) DC (figure 6r–6t).

Figure 6Figure 6Figure 6

Flow cytometry to explore the influence of BiTP on the TME in the 4T1-hPD-L1 tumors. (A–C) The gating strategies for CD8+ T cell, NK cell, and DC in flow cytometry assays. (D-T) The quantification of tumor-infiltrating lymphocyte, CD8+ T cell, CD69+ CD8+ T cell, CD25+ CD8+ T cell, Ki67+ CD8+ T cell, Granzyme-B+ CD8+ T cell, Perforin+ CD8+ T cell, TNF-α+ CD8+ T cell, NK cell, Ki67+ NK cell, Granzyme-B+ NK cell, IFN-γ+ NK cell, Perforin+ NK cell, TNF-α+ NK cell, DC, CD80+ DC, and CD86+ DC. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 mean the significant difference compared with BiTP-treated samples. DC, dendritic cell; TME, tumor microenvironment.

Bulk RNA-seq depicting the TME status

To explore the mechanisms of enhanced antitumor activity of BiTP, we performed bulk RNA-seq using 4T1-hPDL1 tissues. Theoretically, BiTP has the greatest ability among all antibodies to stimulate antitumor immune response. We used BiTP-treated tumors as the baseline to validate whether BiTP has superior immunostimulatory properties to others. Comparing BiTP vs other antibodies, nearly all DEGs were upregulated in BiTP-treated tumors. It is reasonable to assume that these upregulated DEGs might be mainly attributed to BiTP-boosted antitumor immunity. Here we focused on the overlapping DEGs among the three comparisons, which should converge toward immune activation in theory. BiTP-treated tumors had a distinguished transcriptomic profile. DEG analysis identified 2144 (BiTP vs hIgG), 2211 (BiTP vs anti-TGF-β), and 709 (BiTP vs anti-PD-L1) genes, respectively (figure 7A). In all DEGs identified in three comparisons, 598 genes overlapped (566 genes were upregulated and 32 genes were downregulated in BiTP-treated tumors). Enrichment analyses showed the overlapped DEGs were highly associated with immune activation (online supplemental figure S5A–D). As expected, some immune killing-associated genes such as Ifng, Tnf, Gzma, Gzmb were markedly upregulated in BiTP-treated samples. Then, KEGG and GO enrichment analyses based on the identified DEGs were performed. The results showed immune response, innate/adaptive immune response, cytokine-cytokine receptor interaction, chemokine signaling pathway, IL-2/IL-12/IFN-γ/TNF production, T cell functions (TCR signaling, T cell stimulation, activation, proliferation, differentiation, and chemotaxis), NK cell-mediated cytotoxicity, NK cell activation, antigen processing and presentation, DC differentiation were significantly enriched in BiTP-treated samples (figure 7b).

Figure 7Figure 7Figure 7

Bulk RNA-seq to evaluate im

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