Investigating the oxidase-like activity of a Co-Fe Prussian blue analog nanocube prepared in situ and its applications in colorimetric detection of ascorbic acid, alkaline phosphatase, α-glucosidase, and ascorbic acid oxidase

In this work, a Co-Fe Prussian blue analogue nanocube (Co-Fe PBA NC) was prepared in situ by a simple and rapid coprecipitation method at room temperature, and the synthesized nanocube was systematically characterized. The Co-Fe PBA NC was proven to have intrinsic oxidase-like activity, which can catalyze the production of reactive oxygen species in a dissolved oxygen system without the presence of hydrogen peroxide (H2O2), including superoxide anions (O2•−), hydroxyl radical (•OH), and singlet oxygen (1O2). By making full use of the oxidase-activity of Co-Fe PBA NC and using the chromogenic substrate 3, 3', 5, 5'-tetramethylbenzidine (TMB), a colorimetric sensor for the detection of ascorbic acid (AA) and its related biological enzymes’ activities in human serum, including alkaline phosphatase (ALP), α-glucosidase (α-Glu), and ascorbic acid oxidase (AAO), was established. This study provides an oxidase mimetic that can be synthesized by a simple, rapid, and low-cost method, which overcomes the defects of some previously reported nanozymes that may require cumbersome and time-consuming synthesis process, expensive and dependence on professional instruments. In addition, this study provides a new multifunctional colorimetric sensing platform for AA and its related biological enzymes, showing great potential in biological assays.

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