Biomolecules, Vol. 12, Pages 1795: Acyl-Lipid Δ6-Desaturase May Act as a First FAD in Cyanobacteria

Figure 1. Separation of fatty acids methyl esters obtained from total lipids of strain Synechococcus elongatus PCC 7942 by variants (total ion chromatograms): (a) wild type cells; (b) cells expressed the desD (sll026) gene (grey plate) and, supposedly, desaturated FAs at position Δ6.

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Figure 2. Mass-spectra 3-pyridylcarbinyl derivatives of (a) cis,cis-6,9-tetradecadienoic and (b) cis,cis-6,10-heptadecadienoic acids, which were recorded after pre-concentration of FAMEs from the total lipids of Synechococcus cells that expressed the desD gene and were grown in the presence of the potassium salt of cis-17:1Δ10.

Figure 2. Mass-spectra 3-pyridylcarbinyl derivatives of (a) cis,cis-6,9-tetradecadienoic and (b) cis,cis-6,10-heptadecadienoic acids, which were recorded after pre-concentration of FAMEs from the total lipids of Synechococcus cells that expressed the desD gene and were grown in the presence of the potassium salt of cis-17:1Δ10.

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Table 1. FA composition (%) of S. elongatus (WT) and its trasformant that expressed the desD gene.

Table 1. FA composition (%) of S. elongatus (WT) and its trasformant that expressed the desD gene.

FAWTdesD14:00.61.314:1Δ6nd0.514:1Δ91.11.116:055.648.716:1Δ6nd9.716:1Δ935.821.016:2Δ6,9nd7.418:01.43.518:1Δ6nd1.518:1Δ94.13.518:1Δ9,111.40.418:2Δ6,9nd1.4

Table 2. Chromatographic features of FAMEs and mass-spectrometry features of 3-pyridilcarbinyl derivatives of FAs from cell S. elongatus PCC 7942 transformed with the desD (sll0262) gene and grown in presence of potassium salt of cis-10-heptadecenoic acid (17:1Δ10). RT—retention time; RRT—relative retention time to C18:0; ECN—equivalent carbon number; M+—molecular ion peak; amu—atomic mass unit(s).

Table 2. Chromatographic features of FAMEs and mass-spectrometry features of 3-pyridilcarbinyl derivatives of FAs from cell S. elongatus PCC 7942 transformed with the desD (sll0262) gene and grown in presence of potassium salt of cis-10-heptadecenoic acid (17:1Δ10). RT—retention time; RRT—relative retention time to C18:0; ECN—equivalent carbon number; M+—molecular ion peak; amu—atomic mass unit(s).

FART minRRT18:0M+12 amu Gap40 amu Gap14:010.2760.602 14:1Δ610.9260.640317206–218178–21814:1Δ911.1360.653317248–260234–27414:2Δ6,912.320.722315206–218, 246–258178–218, 218–25816:012.960.759 16:1Δ613.8210.810345206–218192–23216:1Δ914.0060.821345248–260234–27417:1Δ1014.7580.865359262–274248–28816:2Δ6,915.2760.895343206–218, 246–258232–272, 192–23217:2Δ6,1016.9560.994357206–218, 260–272178–218, 232–27218:017.0641.000 18:1Δ618.1591.064373220–232206–24618:1Δ918.2641.070373248–260234–27418:1Δ1118.4651.082373276–288248–28818:2Δ6,919.6151.149371206–218, 246–258178–218, 232–272

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