Advances in Pharmacology and Pharmacy Vol. 11(1), pp. 1 - 14
DOI: 10.13189/app.2023.110101
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Snigdha Bhardwaj 1, Sonam Bhatia 2,*
1 I.T.S College of Pharmacy, Murad Nagar, Ghaziabad, Uttar Pradesh (201206), India
2 Vytals Wellbeing India Private Limited, Epitome Tower, DLF Cyber City, Phase 3, Gurugram, 122002, Haryana, India

ABSTRACT

Antimicrobial resistance has raised a serious concern due to the lack of therapeutics to control pathogenic infections. Pseudomonas aeruginosa is considered a multidrug-resistant pathogen and a leading cause of hospital-acquired infections. Anti-virulence approach mainly focuses on targeting bacterial virulence despite its growth and proves effective against such drug-resistant pathogens. In this study, we select a novel flavonoid compound, 7,8-dihydroxyflavone (DHF), which has been reported as a quorum sensing inhibitor (QSI) of P. aeruginosa, performed its in-silico studies to observe its binding affinity and potential interactions with the target protein (2UV0) and further developed it as a nano drug formulation (DHF-NF), and performed its in-vitro and in-vivo evaluation. The prepared DHF-NF was examined for its efficacy in the prevention of wound-associated surface infections of P. aeruginosa in rats. The DHF-NF was prepared by using homogenization and examined for several physical parameters. The prepared DHF-NF had a spherical shape and smooth topology. The microbiological assays demonstrated the inhibitory potential of DHF-NF in a dose-dependent manner. The histological analysis of Pseudomonas infected tissue of rat skin showed significant activity in animal group treated with DHF-NF as compared to control groups. The microbiological and in-vivo evaluation results revealed that the compound, DHF, showed strong inhibitory potential and suggest that the DHF-NF could be utilized as novel anti-QS and anti-biofilm therapeutics against Pseudomonal surface infections.

KEYWORDS
Pseudomonas aeruginosa, Quorum Sensing, Flavonoid Compound, LasR Inhibitor, Nanoemulsion Formulation, Biofilm Inhibition, Surface Infections

Cite This Paper in IEEE or APA Citation Styles
(a). IEEE Format:
[1] Snigdha Bhardwaj , Sonam Bhatia , "Formulation and Biological Evaluation of Quorum Sensing Inhibitor-Based Nanoemulsion against Resistant Pseudomonal Infections," Advances in Pharmacology and Pharmacy, Vol. 11, No. 1, pp. 1 - 14, 2023. DOI: 10.13189/app.2023.110101.

(b). APA Format:
Snigdha Bhardwaj , Sonam Bhatia (2023). Formulation and Biological Evaluation of Quorum Sensing Inhibitor-Based Nanoemulsion against Resistant Pseudomonal Infections. Advances in Pharmacology and Pharmacy, 11(1), 1 - 14. DOI: 10.13189/app.2023.110101.