Verlinsky Y, Kuliev A. Preimplantation diagnosis of common aneuploidies in infertile couples of advanced maternal age. Hum Reprod. 1996;11(10):2076–7.

Article  CAS  PubMed  Google Scholar 

Gleicher N, Orvieto R. Is the hypothesis of preimplantation genetic screening (PGS) still supportable? A review. J Ovarian Res. 2017;10(1):21.

Article  PubMed  PubMed Central  Google Scholar 

Victor AR, Griffin DK, Brake AJ, Tyndall JC, Murphy AE, Lepkowsky LT, Lal A, Zouves CG, Barnes FL, McCoy RC, et al. Assessment of aneuploidy concordance between clinical trophectoderm biopsy and blastocyst. Hum Reprod. 2019;34(1):181–92.

Article  CAS  PubMed  Google Scholar 

Lawrenz B, El Khatib I, Linan A, Bayram A, Arnanz A, Chopra R, De Munck N, Fatemi HM. The clinicians dilemma with mosaicism-an insight from inner cell mass biopsies. Hum Reprod. 2019;34(6):998–1010.

Article  CAS  PubMed  Google Scholar 

Sermon K, Capalbo A, Cohen J, Coonen E, De Rycke M, De Vos A, Delhanty J, Fiorentino F, Gleicher N, Griesinger G, et al. The why, the how and the when of PGS 2.0: current practices and expert opinions of fertility specialists, molecular biologists, and embryologists. Mol Hum Reprod. 2016;22(8):845–57.

Article  PubMed  PubMed Central  Google Scholar 

Gleicher N, Patrizio P, Brivanlou A. Preimplantation genetic testing for aneuploidy - a castle built on sand. Trends Mol Med. 2021;27(8):731–42.

Article  CAS  PubMed  Google Scholar 

Deglincerti A, Croft GF, Pietila LN, Zernicka-Goetz M, Siggia ED, Brivanlou AH. Self-organization of the in vitro attached human embryo. Nature. 2016;533(7602):251–4.

Article  CAS  PubMed  Google Scholar 

Scott RT Jr, Upham KM, Forman EJ, Zhao T, Treff NR. Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not: a randomized and paired clinical trial. Fertil Steril. 2013;100(3):624–30.

Article  PubMed  Google Scholar 

Spinella F, Fiorentino F, Biricik A, Bono S, Ruberti A, Cotroneo E, Baldi M, Cursio E, Minasi MG, Greco E. Extent of chromosomal mosaicism influences the clinical outcome of in vitro fertilization treatments. Fertil Steril. 2018;109(1):77–83.

Article  PubMed  Google Scholar 

Greco E, Minasi MG, Fiorentino F. Healthy babies after intrauterine transfer of mosaic aneuploid blastocysts. N Engl J Med. 2015;373(21):2089–90.

Article  PubMed  Google Scholar 

Viotti M, Victor AR, Barnes FL, Zouves CG, Besser AG, Grifo JA, Cheng EH, Lee MS, Horcajadas JA, Corti L, et al. Using outcome data from one thousand mosaic embryo transfers to formulate an embryo ranking system for clinical use. Fertil Steril. 2021;115(5):1212–24.

Article  CAS  PubMed  Google Scholar 

Treff NR, Marin D. The, “mosaic” embryo: misconceptions and misinterpretations in preimplantation genetic testing for aneuploidy. Fertil Steril. 2021;116(5):1205–11.

Article  PubMed  Google Scholar 

Gazzo E, Pena F, Valdez F, Chung A, Bonomini C, Ascenzo M, Velit M, Escudero E. The Kidscore™ D5 algorithm as an additional tool to morphological assessment and PGT-A in embryo selection: a time-lapse study. JBRA Assist Reprod. 2020;24(1):55–60.

PubMed  PubMed Central  Google Scholar 

Sturmey RG, Bermejo-Alvarez P, Gutierrez-Adan A, Rizos D, Leese HJ, Lonergan P. Amino acid metabolism of bovine blastocysts: a biomarker of sex and viability. Mol Reprod Dev. 2010;77(3):285–96.

Article  CAS  PubMed  Google Scholar 

Pallinger E, Bognar Z, Bodis J, Csabai T, Farkas N, Godony K, Varnagy A, Buzas E, Szekeres-Bartho J. A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer. Sci Rep. 2017;7:39927.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Palini S, Galluzzi L, De Stefani S, Bianchi M, Wells D, Magnani M, Bulletti C. Genomic DNA in human blastocoele fluid. Reprod Biomed Online. 2013;26(6):603–10.

Article  CAS  PubMed  Google Scholar 

Leaver M, Wells D. Non-invasive preimplantation genetic testing (niPGT): the next revolution in reproductive genetics? Hum Reprod Update. 2020;26(1):16–42.

Article  CAS  PubMed  Google Scholar 

Xu J, Fang R, Chen L, Chen D, Xiao JP, Yang W, Wang H, Song X, Ma T, Bo S, et al. Noninvasive chromosome screening of human embryos by genome sequencing of embryo culture medium for in vitro fertilization. Proc Natl Acad Sci USA. 2016;113(42):11907–12.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Khalife D, Abu-Musa A, Khalil A, Ghazeeri G. Towards the selection of embryos with the greatest implantation potential. J Obstet Gynaecol. 2021;41(7):1010–5.

Article  PubMed  Google Scholar 

Wang J, Wei Y, Diao F, Cui Y, Mao Y, Wang W, Liu J. The association between polycystic ovary syndrome and ectopic pregnancy after in vitro fertilization and embryo transfer. Am J Obstet Gynecol. 2013;209(2):e31-139.

Article  Google Scholar 

Cai LB, Qian XQ, Wang W, Mao YD, Yan ZJ, Liu CZ, Ding W, Huang J, Chai DC, Chian RC, et al. Oocyte vitrification technology has made egg-sharing donation easier in China. Reprod Biomed Online. 2012;24(2):186–90.

Article  PubMed  Google Scholar 

Gardner DK, Schoolcraft WB. Culture and transfer of human blastocysts. Curr Opin Obstet Gynecol. 1999;11(3):307–11.

Article  CAS  PubMed  Google Scholar 

Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology. The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod. 2011;26(6):1270–83.

Article  Google Scholar 

Zhang H, Zhao YY, Song J, Zhu QY, Yang H, Zheng ML, Xuan ZL, Wei Y, Chen Y, Yuan PB, et al. Statistical approach to decreasing the error rate of noninvasive prenatal aneuploid detection caused by maternal copy number variation. Sci Rep. 2015;5:16106.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Shen J, Wu W, Gao C, Ochin H, Qu D, Xie J, Gao L, Zhou Y, Cui Y, Liu J. Chromosomal copy number analysis on chorionic villus samples from early spontaneous miscarriages by high throughput genetic technology. Mol Cytogenet. 2016;9:7.

Article  PubMed  PubMed Central  Google Scholar 

Hou Y, Fan W, Yan L, Li R, Lian Y, Huang J, Li J, Xu L, Tang F, Xie XS, et al. Genome analyses of single human oocytes. Cell. 2013;155(7):1492–506.

Article  CAS  PubMed  Google Scholar 

Liu W, Liu J, Du H, Ling J, Sun X, Chen D. Non-invasive pre-implantation aneuploidy screening and diagnosis of beta thalassemia IVSII654 mutation using spent embryo culture medium. Ann Med. 2017;49(4):319–28.

Article  CAS  PubMed  Google Scholar 

Vera-Rodriguez M, Diez-Juan A, Jimenez-Almazan J, Martinez S, Navarro R, Peinado V, Mercader A, Meseguer M, Blesa D, Moreno I, et al. Origin and composition of cell-free DNA in spent medium from human embryo culture during preimplantation development. Hum Reprod. 2018;33(4):745–56.

Article  CAS  PubMed  Google Scholar 

Kuznyetsov V, Madjunkova S, Antes R, Abramov R, Motamedi G, Ibarrientos Z, Librach C. Evaluation of a novel non-invasive preimplantation genetic screening approach. PLoS ONE. 2018;13(5):e0197262.

Article  PubMed  PubMed Central  Google Scholar 

Li P, Song Z, Yao Y, Huang T, Mao R, Huang J, Ma Y, Dong X, Huang W, Huang J, et al. Preimplantation genetic screening with spent culture medium/blastocoel fluid for in vitro fertilization. Sci Rep. 2018;8(1):9275.

Article  PubMed  PubMed Central  Google Scholar 

Rubio C, Rienzi L, Navarro-Sanchez L, Cimadomo D, Garcia-Pascual CM, Albricci L, Soscia D, Valbuena D, Capalbo A, Ubaldi F, et al. Embryonic cell-free DNA versus trophectoderm biopsy for aneuploidy testing: concordance rate and clinical implications. Fertil Steril. 2019;112(3):510–9.

Article  CAS  PubMed  Google Scholar 

Yeung QSY, Zhang YX, Chung JPW, Lui WT, Kwok YKY, Gui B, Kong GWS, Cao Y, Li TC, Choy KW. A prospective study of non-invasive preimplantation genetic testing for aneuploidies (NiPGT-A) using next-generation sequencing (NGS) on spent culture media (SCM). J Assist Reprod Genet. 2019;36(8):1609–21.

Article  PubMed  PubMed Central  Google Scholar 

Kuznyetsov V, Madjunkova S, Abramov R, Antes R, Ibarrientos Z, Motamedi G, Zaman A, Kuznyetsova I, Librach CL. Minimally invasive cell-free human embryo aneuploidy testing (miPGT-A) utilizing combined spent embryo culture medium and blastocoel fluid - towards development of a clinical assay. Sci Rep. 2020;10(1):7244.

Article  CAS  PubMed  PubMed Central  Google Scholar 

Tsuiko O, Zhigalina DI, Jatsenko T, Skryabin NA, Kanbekova OR, Artyukhova VG, Svetlakov AV, Teearu K, Trosin A, Salumets A, et al. Karyotype of the blastocoel fluid demonstrates low concordance with both trophectoderm and inner cell mass. Fertil Steril. 2018;109(6):1127-1134 e1121.

Article  PubMed  Google Scholar 

Huang L, Bogale B, Tang Y, Lu S, Xie XS, Racowsky C. Noninvasive preimplantation genetic testing for aneuploidy in spent medium may be more reliable than trophectoderm biopsy. Proc Natl Acad Sci USA. 2019;116(28):14105–12.

Article  CAS  PubMed  PubMed Central