Sodium carboxymethyl cellulose-based extrafibrillar demineralization to optimize dentin bonding durability

Elsevier

Available online 26 November 2022

Dental MaterialsAuthor links open overlay panelHighlights•

3 % SCMC could demineralize extrafibrillar minerals while preserve intrafibrillar minerals.

The adhesive-dentin bonding durability was enhanced by SCMC as an extrafibrillar demineralization conditioner.

SCMC exhibited a dose-dependent inhibitory effect on rhMMP-2.

AbstractObjective

To investigate the effects of a sodium carboxymethyl cellulose (SCMC)-based extrafibrillar demineralization conditioner on dentin bonding durability and explore the possible mechanisms.

Methods

The SCMC-based extrafibrillar demineralization conditioner was facilely developed by dissolving SCMC into deionized water at an appropriate concentration. A single layer collagen mineralization/demineralization model was designed to visualize extrafibrillar demineralization in detail. Dentin surfaces of human third molars were conditioned with 3 % SCMC or 37 % phosphoric acid (PA). The morphology, composition, and mechanical properties of conditioned dentin from each group were characterized. To evaluate dentin bonding performance, SCMC- and PA- conditioned dentin were applied with adhesive restoration using the dry-bonding technique. The microtensile bond strength (MTBS), interface nanoleakage, and in situ zymography were measured after 24 h of water storage, 10,000 thermocycles, or one month of collagenase aging. The inhibitory effect of SCMC on recombinant human matrix metalloproteinase-2 (rhMMP-2) and cell toxicity were also investigated.

Results

After SCMC conditioning, both demineralization of extrafibrillar minerals and retention of intrafibrillar minerals were observed in the single layer collagen model and the dentin ultrastructure. The mechanical properties of SCMC-conditioned dentin were largely preserved. Compared with PA, SCMC conditioning produced greater MTBS values and less nanoleakage expression after aging. Endogenous gelatinolytic activity was suppressed in SCMC-conditioned dentin. In addition to being nontoxic, the inhibition of rhMMP-2 by SCMC was confirmed to be dose-dependent.

Significance

From the perspective of minimal intervention, the SCMC-based extrafibrillar demineralization conditioner could improve dentin bonding durability, suggesting a promising strategy to extend the service life of adhesive restorations.

Keywords

Dentin bonding

Sodium carboxymethyl cellulose

Extrafibrillar demineralization

Matrix metalloproteinases

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© 2022 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.

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