Authors

Min Ji Lee, Department of Biological science and Bioengineering, Inha University, Incheon 22212, Republic of Korea
Sheik Aliya, Department of Biological science and Bioengineering, Inha University, Incheon 22212, Republic of Korea
Eun-Seon Lee, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Republic of Korea
Tae-Joon Jeon, Department of Biological science and Bioengineering, Inha University, Incheon 22212, Republic of KoreaFollow
Mi-Hwa Oh, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Republic of KoreaFollow
Yun Suk Huh, Department of Biological science and Bioengineering, Inha University, Incheon 22212, Republic of KoreaFollow

Abstract

This study developed a simple and rapid strategic technique to detect ractopamine (chemical growth-promoting agent) in pork. Two highly sensitive and specific gold nanoparticle-based portable sensors, i.e., localized surface plasmon resonance (LSPR) sensors, and lateral flow immunoassay (LFIA) strips were developed to detect veterinary drug residues in food products, that have detrimental effects on humans. Optimization studies were conducted on several sensor devices to improve sensitivity. Each sensor comprised functionalized gold nanoparticles conjugated with ractopamine antibodies. The LSPR sensor chip achieved excellent detection sensitivity = 1.19 fg/mL and was advantageous for quantitative analysis due to its wide dynamic range. On the other hand, LFIA strips provided visual test confirmation and achieved 2.27 ng/mL detection sensitivity, significantly less sensitive than LSPR. The complementary sensors help overcome each other's shortcomings with both the techniques offering ease of use, affordability and rapid diagnosis. Thus, these sensors can be applied on-site for routine screening of harmful drug residues in pork meat. They also provide useful direction for advanced technologies to enhance assay performance for detecting various other food drug contaminants.

Recommended Citation

Lee, Min Ji; Aliya, Sheik; Lee, Eun-Seon; Jeon, Tae-Joon; Oh, Mi-Hwa; and Huh, Yun Suk (2022) "Simple and rapid detection of ractopamine in pork with comparison of LSPR and LFIA sensors," Journal of Food and Drug Analysis: Vol. 30 : Iss. 4 , Article 7.
Available at: https://doi.org/10.38212/2224-6614.3410

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