Topic: Cancer diagnosis and molecular pathology, Gastrointestinal cancer
Category: Original article
MATERIALS AND METHODS: The liver cancer cell line was prepared from Pasteur Institute of Iran and treated with appropriate concentrations of Pogostone. Cytotoxicity was determined by MTT, trypan blue, and lactate dehydrogenase assay. Apoptosis induction was evaluated by diphenylamine assay, Annexin V-FITC staining and a Real-time PCR test. Data were analyzed by SPSS statistical software using Tukey’s test one-way analysis of variance.
RESULTS: After all three time periods, a significant decrease in viability was observed (p <0.05) in a concentration- and time-dependent manner. The cytotoxicity of Pogostone to liver cancer cells was in a concentration- and time-dependent manner. Pogostone significantly induced apoptosis compared to control cells (p<0.05). Treatment of liver cancer cells with Pogostone significantly reduced Bcl-2 gene expression (p<0.05). On the other hand, expression of all three Bax, p53, and caspase 3 genes showed a significant increase after treatment (p<0.05).
CONCLUSIONS: Pogostone had a concentration- and time-dependent toxic effect on liver cancer cells. It induced apoptosis by increasing the Bax to Bcl-2 ratio.
To cite this article Rezazadeh D. Department of Molecular Medicine, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran , Asadi S. Student Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran , Nemati H. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran , Jalili C. Medical Biology Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran WCRJ 2022;
9: e2436
DOI: 10.32113/wcrj_202211_2436
Submission date: 22 Jun 2022
Revised on: 01 Aug 2022
Accepted on: 27 Oct 2022
Published online: 21 Nov 2022
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