Cycloaddition reactions

In 2020, Anton V. Kutasevich et al. unfolded a very interesting deoxygenative reaction procedure where 2-unsubstituted imidazole N-oxides reacted with ethyl cyanoacetate to generate ethyl 2-cyano-2-(1,3-dihydro-2H-imidazole-2-ylidene)acetates in the presence of aldehyde catalyst through a [3 + 2] cycloaddition [16]. Here, imidazole N-oxides behaved as 1,3-dipoles and nucleophiles in Michael-type addition reactions. The optimized reaction conditions were estimated to be a 1:1:1 ratio of 2-unsubstituted imidazole N-oxides as C-nucleophile, ethyl cyanoacetate as C–H acidic electrophile and 4-(methylsulfanyl)benzaldehyde as aldehyde catalyst, DMF as solvent at 100 °C for 5 h. Under the optimized conditions, malononitrile providing the products 4i,j (36–45%), 2-[(4-chlorophenyl)sulfonyl]acetonitrile gave the corresponding products 4k,l (18–51%) and 2-tosylacetonitrile producing the expected product 4h (28%) can also act as the C–H acids in place of ethyl cyanoacetate. But the yields of the products were relatively low. Here, the product 4c was obtained only in 39% yield because of steric effects and the electron-withdrawing nature of the substituents on the ‘N’-atom of the imidazole N-oxides at C-3 position (Scheme 1). Also, with the increase in chain length, the decrease in yield of products 4a,b was observed. After analyzing X-ray crystallography data, the authors revealed the product 4a as E-isomer due to the strong intramolecular H-bonding. It was also observed that the labile acetal group in the product 4g remained unaffected during the reaction process under the standard conditions. Imidazole N-oxides reacted with ethyl cyanoacetate to produce the corresponding products 4a–g in 30–86% yield regardless of electron-withdrawing and electron-donating groups. Various N-substituted imidazole N-oxides were screened and the yields of products were 18–86%. It was detected by NMR studies that the final expected products are found as single isomers. Based on some experimental results, a suitable mechanistic pathway has been proposed (Scheme 1). At first, imidazole N-oxide underwent a [3 + 2] cycloaddition reaction with ylidene 5 to form unstable cycloadduct 6, which then rearomatized followed by ring opening to provide the intermediate 7. Finally, the breaking of the C–C bond of intermediate 7 led to the generation of the expected final product 4a through retro-one reaction. It was also shown that the side product, 1-benzyl-4,5-dimethyl-1,3-dihydro-2H-imidazol-2-one (8) was formed from 1a through simply thermal rearrangement.

Scheme 1: Formation of ethyl 2-cyano-2-(1,3-dihydro-2H-imidazole-2-ylidene)acetate derivatives via [3 + 2] cycloaddition reactions.

Nucleophilic substitution reactions Metal-free coupling reaction through nucleophilic substitution of H-atom (SNH)

In 2020, a C–H/C–Li coupling reaction between 2H-imidazole 1-oxides and pentafluorophenyllithium under transition metal-free conditions was reported by Timofey D. Moseev and co-workers [17]. The reaction proceeds through the nucleophilic substitution of a H-atom (SNH) at the C-5 position of the imidazole N-oxides. This reaction methodology can be considered as suitable process for the syntheses of bioactive imidazole compounds containing (poly)fluoroaryl moieties. Interestingly, in some of the perfluoroaryl-substituted 2H-imidazole products, a push–pull fluorophore system was discovered, which can be useful for the preparation of fluorometric sensor materials because of the solvent-dependent intramolecular charge transfer effect (ICT). Here, two reaction pathways were designed: (A) addition–elimination pathway (SNHAE), where deoxygenation of imidazole 1-oxide resulted in the formation of perfluoroarylated 2H-imidazoles; and (B) addition–oxidation pathway (SNHAO), where without affecting the N–O bond of imidazole 1-oxides, perfluoroarylated 2H-imidazole 1-oxides were obtained as final products. The study of the optimization conditions showed that the highest yields of products were obtained when: (I) in case of path A, 1.0 mmol of the 2,2-dimethyl-4-phenyl-2H-imidazole 1-oxide (9a) was reacted with pentafluorophenyllithium (13) (which was formed in situ from pentafluorobenzene (12)] in the presence of 1.0 mmol AcCl as eliminating agent (EA) in dry THF as solvent at −78 °C; (II) in case of path B, the mixture of 1.0 mmol of 2,2-dimethyl-4-phenyl-2H-imidazole 1-oxide (9a) and 1.0 mmol of pentafluorobenzene (12), 1.1 mmol of n-BuLi as base, 1.5 equiv DDQ as oxidant in dry THF as solvent was refluxed for 4 h. The screening of several 2H-imidazole 1-oxides revealed that the derivatives of 2H-imidazole 1-oxide reacted with pentafluorophenyllithium to provide the expected products in good yields (64–76%) regardless of the presence of either electron-withdrawing or electron-donating groups at the vicinal position of 2H-imidazole and the addition–elimination pathway providing the products 10a–h or addition–oxidation pathway affording the corresponding products 11a–h (Scheme 2). At first, pentafluorophenyllithium (13) which was produced through the reaction between n-BuLi and pentafluorobenzene (12), acted as the nucleophile to attack the C-5 position of 2H-imidazole 1-oxides 9a–h to form the σH-adduct 14. The use of a deoxygenation agent in the mixture led to the formation of the desired products 10a–h via “addition–elimination” (SNH AE, path A) from the adduct 14 with the elimination of the good leaving groups. The eliminating agent (AcCl) led to O-acylation of the intermediate 14 and resulted in deoxygenation through the release of AcOH giving 2H-imidazole derivatives. On the other hand, in case of “addition–oxidation” (SNH AO, path B), the oxidant DDQ picked up a proton from intermediate 14 to provide the targeted products 11a–h.

Scheme 2: C–H/C–Li coupling reaction of 2H-imidazole 1-oxides with pentafluorophenyllithium.

In the next year (2021), the same authors suggested another fascinating deoxygenative transition-metal-free SNH coupling reaction procedure of 2H-imidazole 1-oxides with polyphenols [10]. This process is convenient for producing bioactive bifunctional compounds containing both imidazole and phenolic moieties under mild conditions with two successive steps. The final products were obtained in good to excellent yields (70–95%). However, this methodology can be successfully used only for the polyphenols with at least two OH groups in one ring and is not applicable for monohydroxy group systems, which is the drawback of this method. The reaction conditions were optimized as 2,2-dimethyl-4-phenyl-2H-imidazole 1-oxide (15a) and resorcinol (16a) (1:1), AcCl (1.0 equiv) as activating agent at 0 °C to rt for 0.5 h. The solvents used in this reaction were acetone, a mixture of toluene and acetone (2:1), mixture of chlorobenzene and acetone (2:1), or a mixture of hexachloroacetone and acetone (7:1). Under the optimized conditions, the screening of various polyphenols showed that the yields of products were 70–95% when the mixture of toluene and acetone (2:1) was used as solvent (Scheme 3). Five polyphenols with at least two OH groups gave the corresponding products in 70–95% yields. After that, through the study of the scope of nitrones against phloroglucinol (16e), it was found that the products corresponding to 2H-imidazole 1-oxides bearing either electron-donating groups (18e–h) or electron-withdrawing groups (18i–k) present at both C-2 and C-4 positions were obtained in 72–95% yields (9 examples). It was also shown the versatility of this synthetic approach by providing various examples, few of them such as 18l–o reflected as good to excellent yields (75–95%).

Scheme 3: Transition-metal-free coupling reaction of 2H-imidazole 1-oxides with polyphenols. Reaction conditions: 1 mmol of each substrate at 0 °C to rt for 30 min; atoluene and acetone mixture (2:1) as solvent; bchlorobenzene and acetone mixture (2:1) as solvent; chexachloroacetone and acetone mixture (7:1) as solvent. The isolated yields are given.

Nucleophilic halogenation reactions

In 2015, Evgeny I. Adiulin and co-workers reported a methodology for the deoxygenative nucleophilic halogenation of 2-unsubstituted imidazole N-oxides at the C-2 position using tosyl halogenides (TsHal) as halogen donor in THF as the solvent [18]. In this procedure, the fluoroborate complexes of the N-oxides O-acylated by TsHal and the cine-substitution occurs with the help of a halogen group releasing the tosyl group. Here, 1.0 equiv 2-unsubstituted imidazole N-oxides were refluxed with 1.0 equiv TsHal in 1.0 equiv pyridine that played the role as both the base and the nucleophilic acylation catalyst in THF as solvent for 1 h. In this reaction, the yield for the transformation of 2-chloro-1-(4-methoxyphenyl)-4,5-dimethyl-1H-imidazole (19a) was 80% when 1.0 equiv TsCl was used. 2-Chloro-1H-imidazoles 20b,c and the corresponding brominated products 21a–c using TsBr were also successfully obtained from the reaction medium (Scheme 4). The proposed mechanism proceeded via cine-substitution. The rate-determining step was found to be the nucleophilic attack of the halide ion at the C-2 position of the O-tosylated imidazole 1-oxide. In spite of the difference in rate for the cases of chloride and bromide where bromination occurred at higher rate, N-tosylpyridinium halide was the acylating agent in both cases.

Scheme 4: Halogenation reaction of 2-unsubstituted imidazole N-oxides using tosyl halogenides.

In 2017, M. Hossain and co-workers suggested a quite unique one-pot deoxygenative chlorination reaction procedure of 2-unsubstituted imidazole N-oxides using oxalyl chloride as chlorinating agent to synthesize pharmacologically important 2-chloroimidazoles under solvent- and metal-free conditions [19]. This reaction process is expeditious and proceeds at room temperature providing excellent yields of the products with a wide substrate scope. The optimized conditions were found to be 1.0 mmol of imidazole N-oxide as substrate, 2.0 equiv of oxalyl chloride as chlorinating agent, 1.5 equiv of triethylamine as base at rt for only 10 minutes. Under the optimized conditions, the screening of several imidazole N-oxides showed that in most of the cases, the products were obtained in excellent yields (83–95%, Scheme 5). The derivatives of 2-unsubstituted imidazole 1-oxide bearing electron-withdrawing groups 23d,e (93–95%) present at the C-4 position in the phenyl ring gave higher yields of products than those containing electron-donating groups 23b,c (83–89%), because of the increased electrophilicity in case of electron-withdrawing groups. Imidazole N-oxides bearing naphthyl (23g) and n-butyl (23f) groups at the N-1 position also afforded the desired products in 93% and 95% yields, respectively. Notably, no product was isolated in case of glyoxal monoxime. The plausible reaction mechanism proposed by the authors proceeded through cine-substitution. In the first step, the activation of imidazole 1-oxide 22 by oxalyl chloride led to the generation of imidazolium chloride 24, which transformed into intermediate 25 through nucleophilic substitution. Finally, the acidic hydrogen atom at C-2 position of the imidazole moiety was captured by triethylamine as base to provide the expected product 23.

Scheme 5: Solvent-free chlorination reaction of imidazole N-oxides.

Multicomponent reactions

In 2016, Vitaly S. Mityanov and co-workers unfolded a multicomponent condensation reaction methodology for the C-2 functionalization of 2-unsubstituted imidazole N-oxides using aldehydes and Meldrum’s acid under metal-free and mild reaction conditions [20]. The optimization conditions were estimated to be 1.0 mmol of 1-benzyl-4,5-dimethylimidazole N-oxide as substrate, 1.0 mmol of aldehydes and 1.0 mmol of Meldrum’s acid (26) in 6.0 mL of acetonitrile as solvent at reflux for 5 h. In this operationally simple procedure, the imidazole N-oxide plays the role of a ‘C’-nucleophile when there is no involvement of acid or base catalyst. 1-Benzyl-4,5-dimethylimidazole N-oxide (28) was chosen as the N-oxide substrate to react with Meldrum’s acid (26) and several aldehydes 27. Here, heteroaromatic, aromatic, and aliphatic aldehydes along with formaldehyde were taken into consideration providing the corresponding products in 33–76% yield (Scheme 6). Based on the plausible mechanism proposed by the authors, most likely the reaction began with the nucleophilic addition reaction of Meldrum’s acid (26) and aldehyde 27 resulting in the formation of the electron-deficient enone 30, which then participated in a Michael-type addition reaction with 1,3-dipolar 2-unsubstituted imidazole N-oxide 28 to provide the intermediate 31. In the last step, the final product 29 was generated from the intermediate 31 with formation of the imidazole moiety.

Scheme 6: Multicomponent reaction of imidazole N-oxides 28 with Meldrum’s acid (26) and aldehydes.

In the next year (2017), the same authors reported another convenient metal-free multicomponent synthetic approach for the C-2 functionalization of 2-unsubstituted imidazole 1-oxides using CH-acids and aldehydes [21]. Here, the mixture of 2.0 mmol of imidazole N-oxides 32, 2.0 mmol of aldehyde 33, and 2.0 mmol of barbituric acid 34 was refluxed in the solvent mixture of 5.0 mL of MeCN and 2.0 mL of water for 6 h. Under the optimized conditions, the study of the substrate scope showed that the corresponding condensation products 35a–q were obtained in 29–96% yields (Scheme 7). Various aldehydes, like heteroaromatic, aromatic, aliphatic, and formaldehyde as 37% solutions in water were chosen to react with several 1-arylimidazole N-oxides, 2-unsubstituted 1-alkyl-4,5-dimethylimidazole N-oxides, and 1-alkyl-2,4-unsubstituted imidazole 1-oxides. The selected CH-acids to study the scope of substrates were barbituric acid, dimedone, 4-hydroxy-6-methyl-2H-pyran-2-one, 1,3-dimethylbarbituric acid, 4-hydroxycoumarin, and 1,3-indandione. The nature of the CH-acids and 2-unsubstituted imidazole N-oxides did not affect the yield of products. Interestingly, unstable enones formed from the CH-acids like 4-hydroxy-6-methylpyrone and 4-hydroxycoumarin also successfully gave the desired products 35p and 35q under the standard conditions. The proposed reaction mechanism proceeded through the same pathway as outlined in Scheme 5. The reaction between the aldehyde and C–H acids followed by the attack of the imidazole N-oxides led to the generation of the final product.

Scheme 7: Multicomponent reaction of imidazole N-oxides with CH-acids and aldehydes. Reaction conditions: aThe mixture of 2-unsbstituted imidazole 1-oxide 32 (2 mmol), aldehyde 33 (2 mmol) and CH acid 34 (2 mmol, barbituric acid) was refluxed in H2O (2 mL) and MeCN (5 mL) solvent mixture for 8 h. bThe mixture of imidazole N-oxide 32 (2 mmol), CH-acid 34 (2 mmol, dimedone, 4-hydroxy-6-methyl-2H-pyran-2-one, 1,3-dimethylbarbituric acid, 4-hydroxycoumarin or 1,3-indandione), and aldehyde 33 (2 mmol) in MeCN (5 mL) as solvent was refluxed for 6 h.

In 2020, Valery P. Perevalov et al. introduced one more interesting simple and metal-free three-component condensation methodology for the C-2 functionalization reaction of 2-unsubstituted imidazole 1-oxides with arylglyoxals and CH-acids [11]. This procedure is convenient for the synthesis of pharmacologically important compounds containing furopyranone and furocoumarin scaffolds. With this method, extensive varieties of polyfunctional imidazole 1-oxides can be obtained. Here, 2-unsubstituted imidazole N-oxides were refluxed with several CH-acids and the heteroaromatic or aromatic hydrates of arylglyoxal in MeCN or a mixture of MeCN and water as solvent to give the corresponding condensation products in modest to good yields (31–89%) (Scheme 8). In this reported work, just akin to previously mentioned methodology in Scheme 7, a wide range of imidazole N-oxides and CH-acids like dimedone, barbituric acid, Meldrum’s acid, 4-hydroxycoumarin, hydroxy-6-methylpyranone, and 4-hydroxy-7,7-dimethyl-7,8-dihydro-2H-chromene-2,5(6H)-dione were chosen along with arylglyoxals to investigate the scope of substrates. No products were obtained in case of the acyclic CH-acids like dimethyl malonate, acetylacetone, and ethyl acetoacetate under the same conditions. The reaction follows almost the same mechanistic pathway that was previously mentioned as three-component reaction. However, in this reaction process, 2.0 equiv of CH-acids were needed against arylglyoxals since the electrophilic character of glyoxals is higher than that of aldehydes, generating less stable enones while reacting with CH-acids. First, 1.0 equiv CH-acid was reacted with 1.0 equiv arylglyoxal to generate an intermediate followed by immediate reaction with another 1.0 equiv CH-acids to form another reactive species which reacted with substituted imidazole N-oxide to produce desired products.

Scheme 8: Three-component condensation reaction of imidazole N-oxides, arylglyoxals, and CH-acids 38 (dimedone, barbituric acid, Meldrum’s acid, 4-hydroxycoumarin, hydroxy-6-methylpyranone, or 4-hydroxy-7,7-dimethyl-7,8-dihydro-2H-chromene-2,5(6H)-dione).

Synthesis and trapping of optically active carbene

In 2019, Grzegorz Mlostoń and co-workers disclosed an excellent synthetic route for the preparation of chiral 3-alkoxyimidazol-2-ylidene intermediates [22]. Optically active 2-unsubstituted imidazole N-oxides were converted to carbene intermediates with retaining their stereochemistry. The appearance of the carbene intermediate was verified by trapping reactions with elemental sulfur [23-25], resulting in the generation of non-enolizable imidazole-2-thiones. At first, the alkylation of 2-unsubstituted imidazole N-oxides 40 took place in the presence of an equimolar quantity of benzyl bromide in CH2Cl2 at rt providing the (N-benzyloxy)imidazolium salts 41. The latter underwent deprotonation in the presence of triethylamine in pyridine to generate the carbene intermediates 42 (Scheme 9). After that, the optically active imidazole-2-thiones 43 were obtained through the reaction with elemental sulfur. In CHCl3 solutions, the study of the optical rotation of the isolated products 43a–h did not show any racemization under the standard reaction conditions.

Scheme 9: Synthesis of imidazole-2-thiones containing cyclohexyl-substituents at 3-position.

Thereafter, imidazolium salts 45a–d, obtained from imidazole N-oxides 44a–d, provided the desired optically active 3-butoxyimidazole-2-thiones 47a–d in 66–83% yield using triethylamine in pyridine followed by the treatment of elemental sulfur, through the formation of the optically active intermediate N-butoxyimidazol-2-ylidenes 46 (Scheme 10).

Scheme 10: Preparation of optically active derivatives of 3-butoxyimidazole-2-thione.