Ruthenium(II) polypyridyl complexes with benzothiophene and benzimidazole derivatives: Synthesis, antitumor activity, solution studies and biospeciation

A2780

human ovarian adenocarcinoma cell line

ABC

ATP binding cassette (class of proteins)

ATP

adenosine 5′-triphosphate

cisPt

cisplatin or cis-diamminedichloro platinum(II) (CDDP)

Colo-205

human colon cancer cell line

Colo-320

multidrug resistant human colon cancer cell line

COSY

1H–1H correlated spectroscopy

DAPI

4′,6-diamidine-2′-phenylindole

DAS

decay-associated spectra

DpH

distribution coefficient at the indicated pH

EMEM

Eagle's minimal essential medium

FTIR

Fourier transform infrared spectroscopy

HR-ESI-MS

high resolution electrospray ionization mass spectroscopy

HEPES

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

HMBC

heteronuclear multiple bond coherence

HMQC

heteronuclear multiple-quantum correlation

IC50

half-inhibitory concentration (concentration that reduces by 50% the growth of cells exposed to the tested compounds

K′

conditional stability constant

Ka

proton dissociation constant

MLCT

metal-to-ligand charge transfer

MRC-5

normal human embryonal lung fibroblast

MTT

3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide

ORTEP

Oak Ridge thermal ellipsoid plot

PAMPA

artificial membrane permeability assay

pbi

2-(2-pyridyl)benzimidazole

pbt

2-(2-pyridyl)benzo[b]thiophene

Peff

effective passive permeability

S

thermodynamic solubility

τ

fluorescence lifetime value

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