Impact of tobacco flavoring on oral nicotine consumption in C57BL/6J mice

ElsevierVolume 241, 1 December 2022, 109685Drug and Alcohol DependenceHighlights•

Overall, tobacco flavoring decreased oral nicotine consumption in C57BL6J mice.

Adolescent male mice were more sensitive to the effects.

Some tobacco product flavors may diminish rather than enhance oral nicotine consumption in rodents.

AbstractBackground

The continued use of flavors in tobacco products has been a prominent factor in their popularity, yet little is known regarding their role in nicotine dependence. This study aimed to investigate the impact of tobacco flavoring on oral nicotine consumption in mice using the two-bottle choice (2BC) test and assessed the potential impact of age and sex in their interactions.

Methods

Adolescent and adult male and female C57BL/6J mice were used. First, voluntary consumption of tobacco flavor concentrate from a commercial electronic cigarette liquid vendor (Avail Vapor LLC) was measured; then, the effects of tobacco flavoring in combination with nicotine were examined. In one approach, tobacco flavor concentration was kept constant while nicotine concentration varied, and in the second, nicotine was kept constant while the tobacco flavor concentration varied.

Results

Overall, tobacco flavoring decreased oral nicotine consumption in mice, and its effects were sex- and age-dependent. Although females consumed the tobacco-flavored solution at a slightly higher rate than males, male mice were more sensitive to the effects of the combination (nicotine + tobacco). Furthermore, adolescent mice showed a starker reduction in nicotine consumption in the presence of tobacco flavoring compared to adult mice. This attenuation was most likely due to a basal aversion to the tobacco flavoring itself, thus, creating a negative synergistic effect with nicotine.

Conclusions

Tobacco flavoring increases aversion to nicotine in the 2BC test in C57BL6J mice, suggesting that some flavors may diminish rather than enhance oral nicotine consumption in rodents.

Keywords

Nicotine

Tobacco flavoring

Two-bottle choice

Self-administration

Electronic cigarettes

C57BL6J mice

View full text

© 2022 Elsevier B.V. All rights reserved.

留言 (0)

沒有登入
gif