Comparison of different approaches for direct coupling of solid-phase microextraction to mass spectrometry for drugs of abuse analysis in plasma

Journal of Pharmaceutical Analysis

Available online 9 November 2022

Journal of Pharmaceutical AnalysisHighlights•

Rapid screening and quantitative analysis were achieved by SPME-MS direct coupling.

SPME with different geometries including fiber, blade and probe were studied.

Three SPME-MS techniques including MOI-MS, CBS-MS and PESI-MS has been investigated.

High throughput SPME were achieved by automated 96 Concept system.

The methods showed good analytical performance for drugs of abuse analysis.

Abstract

The direct coupling of solid-phase microextraction (SPME) to mass spectrometry (MS) (SPME-MS) has proven to be an effective method for the fast screening and quantitative analysis of compounds in complex matrices such as blood and plasma. In recent years, our lab has developed three novel SPME-MS techniques: SPME-microfluidic open interface-MS (SPME-MOI-MS), coated blade spray-MS (CBS-MS), and SPME-probe electrospray ionization-MS (SPME-PESI-MS). The fast and high-throughput nature of these SPME-MS technologies makes them attractive options for point-of-care analysis and anti-doping testing. However, all three of these techniques utilize different SPME geometries and were tested with different MS instruments. Lack of comparative data makes it difficult to determine which of these methodologies is the best option for any given application. This work fills this gap by conducting a comprehensive comparison of these three technologies with different SPME devices including SPME fibers, CBS blades, and SPME-PESI probes and SPME-LC-MS for the analysis of drugs of abuse using the same MS instrument. Furthermore, for the first time, we develop different desorption chambers for MOI-MS for coupling with SPME fibers, CBS blades, and SPME-PESI probes, thus illustrating the universality of this approach. In total, 8 analytical methods were developed, with the experimental data showing that all the SPME-based methods provide good analytical performance with R2 of linearities larger than 0.9925, accuracies between 81%-118%, and good precision with an RSD% ≤ 13%.

Keywords

Solid-phase microextraction

Mass spectrometry

Microfluidic open interface

Coated blade spray

Probe electrospray ionization

Drug of abuse

© 2022 The Author(s). Published by Elsevier B.V. on behalf of Xi’an Jiaotong University.

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