Rubus rosifolius (Rosaceae) stem extract induces cell injury and apoptosis in human hepatoma cell line

Keywords

Rubus rosifolius toxicity

Micronucleus test

Comet assay

Cell cycle analysis

Apoptosis analysis

Gene expression

AbbreviationsANOVA

Analysis of variance

BBC3

BCL2 Binding Component 3

CBMN

Cytokinesis-block micronucleus

CDKN1A

Cyclin Dependent Kinase Inhibitor 1A

cDNA

complementary desoxirribonucleic acid

CID

collision induced dissociation

CYP1A2

Citocromo P 450 Family 1 Subfamily A Member 2

CYP3A4

Cytochrome P 450 Family 3 Subfamily A Member 4

CYP2C19

Citocromo P 450 Family 2 Subfamily C Member 19

CYP2D6

Citocromo P 450 Family 2 Subfamily D Member 6

CYP2E1

Citocromo P 450 Family 2 Subfamily E Member 1

DMEM

Gibco Dulbecco's Modified Eagle Medium

DNA

Desoxirribonucleic acid

GAPDH

Glyceraldehyde-3-Phosphate Dehydrogenase

HPLC

high-performance liquid chromatography

LC-MS

liquid chromatography mass spectrometry

mTOR

Mechanistic Target of Rapamycin

MTT

3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide

NFKB

factor nuclear kappa B

NDI

Nuclear Division Index

PBS

phosphate-buffered saline

PCR

Polymerase Chain Reaction

PVDF

polyvinylidene difluoride membrane

RrSE

Rubus rosifolius stem extract

RT-qPCR

Transcription Polymerase Chain Reaction

SCGE

Single-Cell Gel Electrophoresis

TRAF2

TNF receptor associated fator 2

UFSC

Universidade Federal de Santa Catarina

UPLC

ultra performance liquid chromatography

7-AAD

7-aminoactinomycin D;

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