A benzenesulfonic acid-modified organic polymer monolithic column with reversed-phase/hydrophilic bifunctional selectivity for capillary electrochromatography

Journal of Pharmaceutical Analysis

Available online 2 November 2022

Journal of Pharmaceutical AnalysisHighlights•

A novel functional monomer VBS was explored as monolithic column stationary phase.

The prepared column showed excellent separation performances toward various compounds.

The maximum column efficiency can be up to 170000 plates/m.

Satisfactory reproducibility and stability were obtained.

Abstract

Here, a styrene-based polymer monolithic column poly(VBS-co-TAT-co-AHM) with reversed-phase/hydrophilic (RPLC/HILIC) bifunctional separation mode was successfully prepared for capillary electrochromatography by the in situ polymerization of sodium p-styrene sulfonate (VBS) with cross-linkers 3-(Acryloyloxy)-2-hydroxypropyl methacrylate (AHM) and 1,3,5-triacryloylhexahydro-1,3,5-triazine (TAT). The preparation conditions of the monolith were optimized. The morphology and formation of the poly(VBS-co-TAT-co-AHM) monolith were confirmed by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR). The separation performances of the monolith were evaluated systematically. It should be noted that the incorporation of VBS functional monomer can provide π−π interactions, hydrophilic interactions as well as ion-exchange interactions. Hence, the prepared poly(VBS-co-TAT-co-AHM) monolith can achieve efficient separation of thiourea compounds, benzene series, phenol compounds, aniline compounds and sulfonamides in RPLC or HILIC separation modes. The highest theoretical plate number for N,N'-dimethylthiourea reached 1.7 × 105 plates/m. In addition, the poly(VBS-co-TAT-co-AHM) monolithic column showed excellent reproducibility and stability. This novel monolithic column has great application value and potential in CEC.

Keywords

sodium p-styrene sulfona

stationary phase

monolithic column

capillary electrochromatography

bifunctional interaction

© 2022 The Authors. Published by Elsevier B.V. on behalf of Xi’an Jiaotong University.

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