Anticancer effect evaluation of iridium(III) complexes targeting mitochondria and endoplasmic reticulum

Keywords

Iridium(III) complexes

Endoplasmic reticulum stress

Mitochondrial pathway

Apoptosis

Western blot analysis

AbbreviationsBak

homologous antagonist killer

BBIP

2-(7-bromo-2H-benzo[d]imidazole-4-yl)-1H-imidazo[4,5-f][1,10]phenanthroline)

BEL-7402

human hepatocellular

Bax

Bcl-2 associated x protein

CCCP

carbonylcyanide-m-chlorophenylhydrazone

Calcein-AM

calcein-acetoxymethylester

DAPI

4′,6-diamidino-2-phenylindole

Caspase 3

cysteinyl aspartate specific proteinase-3

dmb

4,4′-dimethyl-2,2′-bipyridine

DMEM

Dulbecco's Modified Eagle Medium

dmp

2,9-dimethyl-1,10-phenanthroline

DCHF

2′,7′-dichloro-3,6-fluorandiol

DCHF-DA

2′,7′-dichlorodihy-drofluorescein diacetate

DAMPs

damage-associated molecular patterns

ECL

enhanced chemiluminescence

FITC

fluorescein isothiocyanate

Flu-3 AM

Fluo-3-pentaacetoxymethyl ester

HepG2

human hepatocellular carcinoma

HeLa

human cervical cancer cells

HMSPIP

2-(4-(methylsulfonyl)phenyl)-1H-imidazo[4,5-f][1,10]phenanthroline

IC50

50% inhibition of cell growth

JC-1

5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide

LO2

human normal liver cells

m-TOR

mammalian target of rapamycin

MMP

mitochondrial membrane potential

MitoTracker® Deep Red FM

2′,7′-dichlorodihydro-fluorescein diacetate

MTT

3-(4,5-dimethylthiazole)-2,5-diphenyltetraazolium bromide

PARP

poly ADP-ribose polymerase

PBS

phosphate buffer saline

PCC

Pearson's colocalization coefficients

phen

1,10-phenanthrolineRNase, ribonuclease

PI3K

phosphatidylinositol 3-kinase

PMSF

phenylmethylsulfonyl fluoride

P-mTOR

phosphorylated mammalian target of rapamycin

RIPA 50

mM Tris (pH 7.4), 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate

ROS

reactive oxygen species

RPMI 1640

Roswell Park Memorial Institute 1640

TBST

20 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20, pH 8.0

TFPIP

2-(4′-trifluoromethyl)-[1,1′-biphenyl]-4-yl)-1H-imidazo[4,5-f][1,10]phenanthroline)

Tris

tris(hydroxymethyl)aminomethane

Tween

polyoxyethylene monolaura

留言 (0)

沒有登入
gif