Dietary Protein and Fiber Affect Gut Microbiome and Treg/Th17 Commitment in Chronic Kidney Disease Mice

American Journal of Nephrology

Laboratory Investigation: Research Article

Serrano M.a,b· Srivastava A.c· Buck G.a,b· Zhu B.a,b· Edupuganti L.a,b· Adegbulugbe E.c· Shankaranarayanan D.c· Kopp J.B.d· Raj D.S.c

Author affiliations

aDepartment of Microbiology and Immunology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA
bCenter for Microbiome Engineering and Data Analysis, Virginia Commonwealth University, Richmond, VA, USA
cDivision of Kidney Diseases and Hypertension, George Washington University School of Medicine, Washington, DC, USA
dKidney Disease Section, NIDDK, NIH, Bethesda, MD, USA

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Article / Publication Details

First-Page Preview

Abstract of Laboratory Investigation: Research Article

Received: July 01, 2022
Accepted: August 19, 2022
Published online: November 07, 2022

Number of Print Pages: 6
Number of Figures: 1
Number of Tables: 0

ISSN: 0250-8095 (Print)
eISSN: 1421-9670 (Online)

For additional information: https://www.karger.com/AJN

Abstract

Background: Patients with chronic kidney disease (CKD) have dysbiosis, dysmetabolism, and immune dysregulation. Gut microbiome plays an important role shaping the immune system which is an important modulator of CKD progression. Methods: We compared the effect of a diet low in protein and high in fiber (LP-HF; n = 7) to that of diet rich in protein, but low in fiber (HP-LF; n = 7) on gut microbiome and T-cell commitment in male CKD (Alb/TGF-β1) mice. The gut microbiomes of these mice were subjected to 16S rRNA taxonomic profiling at baseline, 6 weeks and 12 weeks of the study. Results: The LP-HF diet was associated with an increase in Butyricicoccus pullicaecorum BT, a taxon whose functions include those closely related to butyric acid synthesis (Kendall’s W statistic = 180 in analysis of microbiome composition). HP-LF diet was associated with increased abundance of two predominantly proteolytic bacterial strains related to Parabacteroides distasonis (W statistic = 173), Mucispirillum schaedleri, and Bacteroides dorei (W statistic = 192). Pathway analysis suggested that the LP-HF diet induced carbohydrate, lipid, and butyrate metabolism. As compared with HP-LF mice, LP-HF mice had 1.7-fold increase in CD4+Foxp3+Treg cells in spleen and 2.4-fold increase of these cells in peripheral blood. There was an 87% decrease in percentage of CD4+ Th17 + cells in spleen and an 85% decrease in peripheral blood, respectively, in LP-HF mice compared to the HP-LF mice. Conclusion: The LP-HF diet promotes the proliferation of saccharolytic bacteria and favors T-cell commitment toward Treg cells in a CKD mouse of model. Clinical significance of the finding needs to be further investigated.

© 2022 S. Karger AG, Basel

References Amdur RL, Feldman HI, Dominic EA, Anderson AH, Beddhu S, Rahman M, et al. Use of measures of inflammation and kidney function for prediction of atherosclerotic vascular disease events and death in patients with CKD: findings from the CRIC Study. Am J Kidney Dis. 2019;73(3):344–53. Tan TG, Sefik E, Geva-Zatorsky N, Kua L, Naskar D, Teng F, et al. Identifying species of symbiont bacteria from the human gut that, alone, can induce intestinal Th17 cells in mice. Proc Natl Acad Sci U S A. 2016 Dec 13;113(50):E8141–50. Mozes MM, Böttinger EP, Jacot TA, Kopp JB. Renal expression of fibrotic matrix proteins and of transforming growth factor-beta (TGF-beta) isoforms in TGF-beta transgenic mice. J Am Soc Nephrol. 1999 Feb;10(2):271–80. Fettweis JM, Serrano MG, Sheth NU, Mayer CM, Glascock AL, Brooks JP, et al. Species-level classification of the vaginal microbiome. BMC Genomics. 2012;13 Suppl 8(Suppl 8):S17. Mandal S, Van Treuren W, White RA, Eggesbø M, Knight R, Peddada SD. Analysis of composition of microbiomes: a novel method for studying microbial composition. Microb Ecol Health Dis. 2015;26:27663. Mitch WE. Dietary therapy in uremia: the impact on nutrition and progressive renal failure. Kidney Int Suppl. 2000 Apr 1;57:S38–43. Asnicar F, Berry SE, Valdes AM, Nguyen LH, Piccinno G, Drew DA, et al. Microbiome connections with host metabolism and habitual diet from 1, 098 deeply phenotyped individuals. Nat Med. 2021 Feb 1;27(2):321–32. Eeckhaut V, Wang J, Van Parys A, Haesebrouck F, Joossens M, Falony G, et al. The probiotic Butyricicoccus pullicaecorum reduces feed conversion and protects from potentially harmful intestinal microorganisms and necrotic enteritis in broilers. Front Microbiol. 2016 Sep 21;7:1416. Loy A, Pfann C, Steinberger M, Hanson B, Herp S, Brugiroux S, et al. Lifestyle and horizontal gene transfer-mediated evolution of Mucispirillum schaedleri, a core member of the murine gut microbiota. mSystems. 2017;2(1):e00171–16. Langille MGI, Zaneveld J, Caporaso JG, McDonald D, Knights D, Reyes JA, et al. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences. Nat Biotechnol. 2013 Sep;31(9):814–21. Solymos P, Stevens MHH, Wagner H. Vegan: community ecology package. 2011 Jan 1. Yoshida N, Emoto T, Yamashita T, Watanabe H, Hayashi T, Tabata T, et al. Bacteroides vulgatus and Bacteroides dorei reduce gut microbial lipopolysaccharide production and inhibit atherosclerosis. Circulation. 2018 Nov 27;138(22):2486–98. Xu J, Mahowald MA, Ley RE, Lozupone CA, Hamady M, Martens EC, et al. Evolution of symbiotic bacteria in the distal human intestine. PLoS Biol. 2007 Jul;5(7):e156. Sakamoto M, Benno Y. Reclassification of Bacteroides distasonis, Bacteroides goldsteinii and Bacteroides merdae as Parabacteroides distasonis gen. nov., comb. nov., Parabacteroides goldsteinii comb. nov. and Parabacteroides merdae comb. nov. Int J Syst Evol Microbiol. 2006 Jul;56(Pt 7):1599–605. Dang EV, Barbi J, Yang HY, Jinasena D, Yu H, Zheng Y, et al. Control of T(H)17/T(reg) balance by hypoxia-inducible factor 1. Cell. 2011 Sep 2;146(5):772–84. Shankaranarayanan D, Raj DS. Gut microbiome and kidney disease: reconciling optimism and skepticism. Clin J Am Soc Nephrol. 2022 Jul 6. Epub ahead of print. Gao B, Jose A, Alonzo-Palma N, Malik T, Shankaranarayanan D, Regunathan-Shenk R, et al. Butyrate producing microbiota are reduced in chronic kidney diseases. Sci Rep. 2021 Dec 7;11(1):23530. Smith PM, Howitt MR, Panikov N, Michaud M, Gallini CA, Bohlooly-Y M, et al. The microbial metabolites, short-chain fatty acids, regulate colonic Treg cell homeostasis. Science. 2013 Aug 2;341(6145):569–73. Muranski P, Restifo NP. Essentials of Th17 cell commitment and plasticity. Blood. 2013;121(13):2402–14. Arpaia N, Campbell C, Fan X, Dikiy S, van der Veeken J, deRoos P, et al. Metabolites produced by commensal bacteria promote peripheral regulatory T-cell generation. Nature. 2013 Dec 19;504(7480):451–5. Article / Publication Details

First-Page Preview

Abstract of Laboratory Investigation: Research Article

Received: July 01, 2022
Accepted: August 19, 2022
Published online: November 07, 2022

Number of Print Pages: 6
Number of Figures: 1
Number of Tables: 0

ISSN: 0250-8095 (Print)
eISSN: 1421-9670 (Online)

For additional information: https://www.karger.com/AJN

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