Acute oral toxicity of damnacanthal and its anticancer activity against colorectal tumorigenesis

MTT

3-(4,5-Dimethythiazol-2-yl)− 2,5-diphenyltetrazolium bromide

DPBS

Dulbecco’s phosphate buffered saline

PMSF

Phenylmethanesulfonyl fluoride

VLC

Vacuum liquid chromatographic method

TLC

Thin layer chromatography

FTIR

Fourier transform infrared spectroscopy

NMR

Nuclear magnetic resonance spectroscopy

IC50

Half-maximal inhibitory concentration

TBST

Tris-buffered saline containing 0.05 % Tween 20

F20

5-Fluorouracil at 20 mg/kg

D20

Damnacanthal at 20 mg/kg

D40

Damnacanthal at 40 mg/kg

AST

Aspartate aminotransferase

ALT

Alanine aminotransferase

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