The Effect Of Temperature On Age Estimation Of Semen Stains On Porous Versus Non-Porous Surfaces Using Messenger Ribonucleic Acid Measurement

Abstract

Background: While mRNA can be used to identify the type of a body fluid, its degradation can also give some indication of the time interval since it was deposited. This study was conducted to evaluate the effect of temperature on the age estimation of human semen stains using mRNA deposited on porous versus non-porous surfaces at different time intervals. Methods: Ten semen samples were applied on two different media (glass and cotton) and exposed to three different temperatures (4°C, room temperature, 40°C) and examined at three time intervals (0, 45, and 90 days). The semen-specific mRNA markers PRM1 and PRM2 were quantitatively assessed along with a reference gene, beta-actin, using reverse transcription-quantitative polymerase chain reaction. Results: Mean Cq values of mRNA markers (PRM1 and PRM2) and the reference gene (beta-actin) increased with time of storage at different temperatures on both examined media. The mean quantification cycle (Cq) values of PRM2 were lower than PRM1, indicating that the levels of PRM2 marker in semen stain were higher than those of PRM1 marker. However, the mean Cq values of PRM2 at each time interval were not significantly different between temperatures, while PRM1 showed statistically significant differences in mean Cq values between temperatures at day 45 on both media. Conclusion: These results indicate that PRM2 can serve as a reliable mRNA marker to estimate the time since deposition of semen stain at different temperatures on two different media.

Keywords: Semen age PRM1 PRM2 temperatures media mRNA RT-qPCR References

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