Prediction of promiscuous epitopes in ORF2 of Hepatitis E virus: an In-Silico approach

Noor Samavia Parvaiz Fahed Waheed Yasir Anwar Tasneem Nasreen Syeda

Keywords: Virology; gastrointestinal disease.

Abstract

Background: Vaccine development against emerging infections is essentially important for saving people from increasing viral infections. In developing countries, Hepatitis E (HEV) is a common infection affecting millions of people worldwide. Based on In-silico analysis, different approaches have been targeted.

Objectives: Rationale of this study is to design an epitope-based vaccine candidates with the help of immunoinformatics that can predict promiscuous B-cell and T-cell epitopes of the most antigenic HEV-ORF2 capsid protein.

Materials & Methods: This study suggests potential T-cell and B-cell epitopes of the highly antigenic HEV ORF2 capsid protein while using various In-silico tools such as NCBI-BLAST, Expassy, CLC workbench, Ellipro and Discotope.

Results: Potential antigenic and immunogenic CD8+ T-cell epitopes were predicted from the global consensus sequence of ORF2-HEV. Furthermore, twenty-two linear B-cell epitopes were predicted. Among these, “SLGAGPV” at position 587-593 and “LEFRNLTPGNTNTRVSRYSS” at position 306-325 were most antigenic with antigenicity score 1.4206 and 1.3600 respectively. Discontinuous B-cell epitopes were found by three-dimensional capsid protein structure. Epitopes predicted in this study reveal high antigenicity and promiscuity for HLA classes.

Conclusion: Collectively, our data suggests promiscuous epitopes that can potentially acts as new candidates for the design of HEV peptide vaccine.

Keywords: Virology; gastrointestinal disease.

A.    AFRICAN HEALTH SCIENCES OPEN ACCESS POLICY

While African Health Sciences has been freely accessible online there have been questions on whether it is Open Access or not. We wish to clearly state that indeed African Health Sciences is Open Access. There are key issues regarding Open Access needing clarification for avoidance of doubt:

1.      Henceforth, papers in African Health Sciences will be published under the CC BY (Creative Commons Attribution License) 4.0 International. See details on https://creativecomons.org/)2.      The copyright owners or the authors grant the 3rd party (perpetually and in advance) the right to disseminate, reproduce, or use the research papers in part or in full, format/medium as long as:No substantive errors are introduced in the processAttribution of authorship and correct citation details are givenThe referencing details are not changed.

Should the papers be reproduced in part, this must be clearly stated.

3.      The papers will be freely and universally accessible online in an easily readable format such as XML in at least one widely recognized open access repository such as PUBMED CENTRAL.

B. ABRIDGED LICENCE AGREEMENT BETWEEN AUTHORS AND African Health Sciences

I submitted my manuscript to African Health Sciences and would like to affirm that:

1.0  I am authorized by my co-authors to enter into these arrangements.

2.0 I guarantee, on behalf of self and co-authors:

That the paper is original, and has not been published in any other peer-reviewed journal; nor is it under consideration by other journal (s). It does not infringe existing copyright or any other person’s rights

 

That we are/I am the sole author(s) of the paper and with authority to enter into this agreement. My granting rights to African Health Sciences is not in breach of any other obligation

 

That the paper contains nothing unlawful, or libelous. Nor anything that would constitute a breach of contract, confidence or commitment given to secrecy, if published

 

That I/we have taken care to ensure the integrity of the article.

3.0  I and all co-authors, agree that the paper, if accepted for publication, shall be licensed under the Creative Commons Attribution License 4.0. (see https://creativecommons.org/)

留言 (0)

沒有登入
gif